ExoBrite™ Calnexin Western Antibody

ExoBrite™ Calnexin Western Antibody is validated by Biotium for detection of calnexin, an endoplasmic reticulum protein (ER) that is not found in extracellular vesicles (EVs). The antibody serves as a negative control to assess the purity of isolated EV extracts. The antibody is offered as a conjugate to ExoBrite™ 770/800 near-infrared fluorescent dye, which offers greater signal-to-noise than visible light fluorescent dyes for western blotting.

• Validated for detection of ER protein calnexin by fluorescent western blot
• Serves as a negative control for assessing purity of isolated EV extracts
• Bright signal and low background
• Available in near-infrared ExoBrite™ 770/800.

EVs, including exosomes, are lipid-bound vesicles that are released from cells. EVs display specific surface proteins and can carry nucleic acids and other cargo, allowing them to transfer biological information between cells in different parts of the body. Therefore EVs are increasingly studied for their potential use in drug delivery and medical diagnostic applications. The most common proteins used as EV markers are CD9, CD63, and CD81, members of the tetraspanin family. Tetraspanins are plasma membrane proteins with many proposed functions, including activation and sorting of other membrane proteins. They are also thought to play a role in the targeting of proteins to multivesicular bodies (MVBs) and exosomes. These tetraspanins are broadly expressed on many cell types and can therefore be detected on many types of EVs, but their expression levels vary depending on the cell type of origin.

EV antibodies you can trust

Other commercially available antibodies for tetraspanin proteins CD9, CD63, and CD81 are generally not validated for isolated EVs and may require tedious optimization for your EV prep and staining protocol. ExoBrite™ Western Antibody Conjugates were validated to offer bright signal and low background of EV markers in EV extracts.

If you are using secondary antibodies for western detection, Biotium also offers unconjugated recombinant antibodies against CD9, CD63, and CD81. ExoBrite™ Flow Antibody Conjugates are also available for optimal detection of CD9, CD63, and CD81 EV markers by flow cytometry.

For general EV staining, Biotium offers ExoBrite™ stains conjugated to cholera toxin B (CTB), wheat germ agglutinin (WGA), and Annexin V. These stains are specially formulated for bright and specific detection of isolated EVs by flow cytometry. These ExoBrite™ stains may also be combined with antibody staining, for multi-parameter analysis.

Biotium also provides ExoBrite™ STORM Antibodies against CD9, CD63, and CD81, as well as ExoBrite™ STORM CTB EV Stains that use CF® Dyes engineered specifically for high-performance super-resolution imaging by STORM.

ExoBrite™ Western Antibody Conjugates