ExoBrite™ CD47 Flow Antibody

ExoBrite™ CD47 Flow Antibody is validated by Biotium for optimal detection of human and mouse CD47 (integrin-associated protein) in purified or bead-bound EVs by flow cytometry. The antibody conjugates offer exceptional signal-to-noise and include color options for the Pacific Blue™, FITC, PE, and APC channels.

• Proprietary buffer formulation for enhanced signal-to-noise
• Optimal detection of CD47 (integrin-associated protein) by flow cytometry
• Validated for purified or bead-bound EVs
• Bright signal and low background
• Available in 4 colors

CD47 is a cell surface transmembrane protein with broad expression, and can often be found on extracellular vesicles (EVs). CD47 contributes to cell adhesion, integrin activation, and immune evasion via binding to SIRP (signal regulator protein) and beta3 integrins CD51/CD61 and CD41/CD61. CD47 is often up-regulated on cancer cells and is sometimes used as a tumor cell-derived marker on EVs. EVs expressing CD47 can cross-dress onto cell surfaces, where they engage signal regulatory protein alpha (SIRPα) to block phagocytosis.

Biotium also offers validated ExoBrite™ Flow Antibodies for tetraspanin proteins CD9, CD63, CD81 and other EV markers.

EV antibodies you can trust

Other commercially available antibodies against mouse and human tetraspanin proteins CD9, CD63, and CD81 are generally not validated for isolated EVs and may require tedious optimization for your EV prep and staining protocol. The antibodies and dye labels of ExoBrite™ Flow Antibody Conjugates were carefully selected and validated for robust detection of isolated EVs. In addition, the antibodies are provided in a proprietary buffer formulation for reduced antibody aggregation and brighter EV staining for optimal accuracy and signal-to-noise. Single-color and 3-color ExoBrite™ CD9/CD63/CD81 Antibody Cocktails for human tetraspanin proteins are also available for high-coverage EV staining or EV phenotyping, respectively.

For general EV staining, Biotium's ExoBrite™ True EV Membrane Stains offer unparalleled coverage of EVs in a sample and address issues of dye aggregation often seen with PKH and other common membrane dyes. Biotium also offers optimized ExoBrite™ EV Surface stains conjugated to cholera toxin B (CTB), wheat germ agglutinin (WGA), and Annexin V. These stains are specially formulated for bright and specific detection of isolated EVs by flow cytometry. These ExoBrite™ EV Surface stains may also be combined with antibody staining, for multi-parameter analysis.

Biotium offers conjugated ExoBrite™ Western Antibodies against human CD9, CD63, and CD81 designed for optimal detection in EV extracts by fluorescent western blot.

Note: In our testing, we have found that ExoBrite™ 490/515 dye may bind to streptavidin coated surfaces or beads if free biotin binding sites are not blocked. We recommend performing a biotin blocking step after binding your biotinylated capture antibody to streptavidin beads or surfaces when using ExoBrite™ 490/515 conjugates. Alternatively, consider using a different ExoBrite™ dye for staining EVs captured on streptavidin beads or surfaces.

ExoBrite™ Flow Antibody Conjugates

ExoBrite™ Flow Anti-Human CD9/CD63/CD81 Antibody Cocktails