ExoBrite™ IgG1 Isotype Control Flow Antibody
Non-reactive mouse antibody that serves as a negative control for ExoBrite™ Flow Antibodies when detecting purified or bead-bound EVs by flow cytometry.
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Product Description
ExoBrite™ IgG1 Isotype Control Flow Antibody has been found to not react with any target in human cells and have the same isotype as the ExoBrite™ Flow Antibodies against tetraspanin proteins CD9, CD63, and CD81. The antibody is available with the same conjugates as ExoBrite™ Flow Antibodies for the Pacific Blue™, FITC, PE, and APC channels.
- Non-reactive IgG1 mouse antibody
- Serves as a isotype negative control for ExoBrite™ Flow Antibodies
- Available in 5 colors
Extracellular vesicles (EVs), including exosomes, are lipid-bound vesicles that are released from cells. EVs display specific surface proteins and can carry nucleic acids and other cargo, allowing them to transfer biological information between cells in different parts of the body. Therefore EVs are increasingly studied for their potential use in drug delivery and medical diagnostic applications. The most common proteins used as EV markers are CD9, CD63, and CD81, members of the tetraspanin family. Tetraspanins are plasma membrane proteins with many proposed functions, including activation and sorting of other membrane proteins. They are also thought to play a role in the targeting of proteins to multivesicular bodies (MVBs) and exosomes. These tetraspanins are broadly expressed on many cell types and can therefore be detected on many types of EVs, but their expression levels vary depending on the cell type of origin.
EV antibodies you can trust.
Other commercially available antibodies for tetraspanin proteins CD9, CD63, and CD81 are generally not validated for isolated EVs and may require tedious optimization for your EV prep and staining protocol. ExoBrite™ Flow Antibody Conjugates were validated to offer bright signal and low background of EV markers in purified and bead-bound EVs.
For general EV staining, Biotium offers ExoBrite™ stains conjugated to cholera toxin B (CTB), wheat germ agglutinin (WGA), and Annexin V. These stains are specially formulated for bright and specific detection of isolated EVs by flow cytometry. These ExoBrite™ stains may also be combined with antibody staining, for multi-parameter analysis.
Biotium also offers conjugated ExoBrite™ Western Antibodies against CD9, CD63, and CD81 designed for optimal detection in EV extracts by fluorescent western blot.
ExoBrite™ Flow Antibody Conjugates
Antibody | Ex/Em | Detection Channel | Size | Catalog No. |
---|---|---|---|---|
ExoBrite™ 410/450 CD9 Flow Antibody | 416/452 nm | Pacific Blue ™ | 25 tests | P003-410-125 |
100 tests | P003-410-500 | |||
ExoBrite™ 490/515 CD9 Flow Antibody | 490/516 nm | FITC | 25 tests | P003-490-125 |
100 tests | P003-490-500 | |||
ExoBrite™ 560/585 CD9 Flow Antibody | 562/584 nm | PE | 25 tests | P003-560-125 |
100 tests | P003-560-500 | |||
ExoBrite™ 650/665 CD9 Flow Antibody | 652/668 nm | APC | 25 tests | P003-650-125 |
100 tests | P003-650-500 | |||
ExoBrite™ R-PE CD9 Flow Antibody | 496, 546, 565/578 | PE | 25 tests | P003-RPE-125 |
100 tests | P003-RPE-500 | |||
ExoBrite™ 410/450 CD63 Flow Antibody | 416/452 nm | Pacific Blue ™ | 25 tests | P004-410-125 |
100 tests | P004-410-500 | |||
ExoBrite™ 490/515 CD63 Flow Antibody | 490/516 nm | FITC | 25 tests | P004-490-125 |
100 tests | P004-490-500 | |||
ExoBrite™ 560/585 CD63 Flow Antibody | 562/584 nm | PE | 25 tests | P004-560-125 |
100 tests | P004-560-500 | |||
ExoBrite™ R-PE CD63 Flow Antibody | 496, 546, 565/578 | PE | 25 tests | P004-RPE-125 |
100 tests | P004-RPE-500 | |||
ExoBrite™ 410/450 CD81 Flow Antibody | 416/452 nm | Pacific Blue ™ | 25 tests | P005-410-125 |
100 tests | P005-410-500 | |||
ExoBrite™ 490/515 CD81 Flow Antibody | 490/516 nm | FITC | 25 tests | P005-490-125 |
100 tests | P005-490-500 | |||
ExoBrite™ 560/585 CD81 Flow Antibody | 562/584 nm | PE | 25 tests | P005-560-125 |
100 tests | P005-560-500 | |||
ExoBrite™ R-PE CD81 Flow Antibody | 496, 546, 565/578 | PE | 25 tests | P005-RPE-125 |
100 tests | P005-RPE-500 | |||
ExoBrite™ 410/450 IgG1 Isotype Control Flow Antibody | 416/452 nm | Pacific Blue ™ | 25 tests | P008-410-125 |
100 tests | P008-410-500 | |||
ExoBrite™ 490/515 IgG1 Isotype Control Flow Antibody | 490/516 nm | FITC | 25 tests | P008-490-125 |
100 tests | P008-490-500 | |||
ExoBrite™ 560/585 IgG1 Isotype Control Flow Antibody | 562/584 nm | PE | 25 tests | P008-560-125 |
100 tests | P008-560-500 | |||
ExoBrite™ R-PE IgG1 Isotype Control Flow Antibody | 496, 546, 565/578 | PE | 25 tests | P008-RPE-125 |
100 tests | P008-RPE-500 | |||
ExoBrite™ 650/665 IgG1 Isotype Control Flow Antibody | 652/668 nm | APC | 25 tests | P003-650-125 |
100 tests | P003-650-500 |