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CD15 / FUT4 / Lewis-X Monoclonal Mouse Antibody (FUT4/815 + BRA-4F1)

CD15 plays a role in mediating phagocytosis, bactericidal activity, and chemotaxis.

Product Attributes

Antibody number

0562

Reactivity (target)

FUT4, CD15, Lewis-x

Antibody type

Primary

Host species

Mouse

Clonality

Monoclonal

Clone

FUT4/815 + BRA-4F1

Isotype

IgM

Molecular weight

~220 kDa (Glycoprotein)

Human gene symbol

FUT4

Entrez gene ID

2526

SwissProt

P22083

Unigene

654379

Immunogen

Purified human neutrophils (FUT4/815); Myelomonocytic leukemia cells (BRA-4F1)

Cellular localization

Membrane/cell surface, Perinuclear

Species reactivity

Human

Applications

Immunofluorescence, Immunohistology (formalin), Flow cytometry

Application notes

Immunohistology (formalin), Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes, Immunofluorescence 0.5-1 ug/mL, Flow Cytometry 0.5-1 ug/million cells/0.1 mL, Optimal dilution for a specific application should be determined by user

Positive control

U937 cells, Reed-Sternberg's cells in Hodgkin's lymphoma

Shipping condition

Room temperature

Storage Conditions

Store at 2 to 8 °C, Note: store BSA-free antibodies at -10 to -35 °C

Shelf life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Regulatory status

For research use only (RUO)

Research areas

Immunology

Cell/tissue expression

Granulocytes

Tumor expression

Leukemia/lymphoma

Conjugation
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Product Description

CD15 plays a role in mediating phagocytosis, bactericidal activity, and chemotaxis. It is present on >95% of granulocytes including neutrophils and eosinophils and to a lesser degree on monocytes. In addition, CD15 is expressed in Reed-Sternberg cells and some epithelial cells. CD15 antibody is very useful in the identification of Hodgkin’s disease. CD15 is occasionally expressed in large cell lymphomas of both B and T phenotypes which otherwise have a quite distinct histological appearance.

This antibody is available purified with BSA/azide at 200 ug/mL, or BSA/azide-free at 1 mg/mL.

References

Hsu et. al. Amer J Clin Pathol 82: 29, 1984. | Pinkus et. al. Am J Pathol 119: 244, 1985. |

 

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