Monoclonal anti CD57 / B3GAT1 (HNK 1)

BNUB0136-0
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Product Description

Anti-CD57 marks a subset of lymphocytes known as natural killer (NK) cells. Follicular center cell lymphomas often contain many NK cells within the neoplastic follicles. Anti-CD57 also stains neuroendocrine cells and their derived tumors, including carcinoid tumor and medulloblastoma. Anti-CD57 can also be useful in separating type B3 thymoma from thymic carcinoma when combined with a panel that includes antibodies against GLUT1, CD5, and CEA.

Abo T et. al. J Immunol, 1982, 129(4):1758-61. | Abo T et al. J Immunology, 1982, 129:1752-7

Additional Information

Conjugation

Purified with BSA, 0.2 mg/mL, Purified, BSA-free, 1 mg/mL, CF405S, CF405M, CF488A, CF543, CF555, CF568, CF594, CF640R, CF647, CF660R, CF680, CF680R, CF770, Biotin, R-PE, APC, Per-CP, Alkaline Phosphatase, HRP

Size

50 uL, 100 uL, 250 uL, 500 uL

Supplied As

Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide, Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA

Antibody type

Primary

Host species

Mouse

Reactivity (target)

CD57 / B3GAT1 (HNK-1)

Clonality

Monoclonal

Isotype

IgM, kappa

Clone

HNK-1

Molecular weight

~110 kDa

Synonyms
Entrez gene ID

27087

SwissProt

Q9P2W7

Unigene

381050

Immunogen

Human peripheral blood mononuclear cells

Species reactivity

Human

Applications

Immunofluorescence, Immunohistology (formalin)

Cellular localization

Cell surface

Application notes

Does not react with rat; others not known, Immunofluorescence 0.5-1 ug/mL, Immunohistology formalin-fixed 2-4 ug/mL for 30 minutes at RT, Optimal dilution for a specific application should be determined by user, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes

Positive control

CCRF-CEM Cells. Lymph node or Tonsil

MSDS (PDF):MSDS Primary antibodies

Protocol (PDF): Protocols for antibody-based detection

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