Monoclonal anti CDw60 (UM4D4)

BNUB0481-0
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Product Description

This MAb reacts with 9-O-acetylated disialosyl group linked to the glycoprotein CD60w. CDw60 is a carbohydrate antigen present on gangliosides, platelets and a subset of CD4+, CD8+peripheral T-cells, but not on B-cells, monocytes or granulocytes. CDw60 is involved in the co-stimulation of T-cells.

Fox DA et. al. in Knapp W (ed) Leucocyte Typing IV 1989; pp364-366 (Oxford University Press, London).2. Fox DA et. al. Immunol Invest 30(2):67-85, 2001

Additional Information

Conjugation

Purified with BSA, 0.2 mg/mL, Purified, BSA-free, 1 mg/mL, CF405S, CF405M, CF488A, CF543, CF555, CF568, CF594, CF640R, CF647, CF660R, CF680, CF680R, CF770, Biotin, R-PE, APC, Per-CP, Alkaline Phosphatase, HRP

Size

50 uL, 100 uL, 250 uL, 500 uL

Supplied As

Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide, Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA

Antibody type

Primary

Host species

Mouse

Reactivity (target)

CDw60 (UM4D4)

Clonality

Monoclonal

Isotype

IgM

Clone

UM4D4

Molecular weight

Not Known

Synonyms

9-O-acetyl GD3; CD60

Entrez gene ID

Not Known

SwissProt

Not Known

Unigene

Not Known

Immunogen

Human rheumatoid synovial T cell line ST-1

Species reactivity

Human

Applications

Immunohistology (frozen), Flow cytometry, Immunofluorescence

Cellular localization

Cell Surface & Cytoplasmic

Application notes

Flow Cytometry (0.5-1 ug/million cells/0.1 mL), Immunofluorescence 0.5-1 ug/mL, Immunohistology Frozen 0.5-1.0 ug/mL for 30 minutes at RT, Optimal dilution for a specific application should be determined by user

Positive control

HL-60, Jurkat, MG63, HUT-78, K562, YT, U937, Hep-G2 cells, Tonsil

MSDS (PDF):MSDS Primary antibodies

Protocol (PDF): Protocols for antibody-based detection

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Please note that products with recommended storage at 4°C or -20°C may ship at ambient temperature. This will not affect product performance. When you receive the product, place it under the recommended storage conditions.

More information about product handling and shelf life