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X-Gal

X-gal (5-Bromo-4-chloro-3-indolyl-β-galactopyranoside) is the most widely used chromogenic substrate for β-galactosidase. Enzyme hydrolysis of the substrate yields a dark blue colored precipitate (maximum absorbance 615 nm).

Product Attributes

CAS number

7240-90-6

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100 mg
1 g
5 g
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Product Description

X-gal (5-Bromo-4-chloro-3-indolyl-β-galactopyranoside) is the most widely used chromogenic substrate for β-galactosidase. Enzyme hydrolysis of the substrate yields a dark blue colored precipitate (maximum absorbance 615 nm).

  • White solid soluble in DMSO
  • Store desiccated at -20°C and protect from light
  • C14H15BrClNO6
  • MW: 408.6
  • [7240-90-6]

 

References

1. Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory, B. 14, p 186 (1989).

 

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    Purple-β-D-Gal

    Purple-β-D-Gal (5-Iodo-3-indolyl-β-D-galactopyranoside) is a chromogenic substrate for β-galactosidase. Similar to X-gal, but generates a purple (λmax ~575 nm) colored precipitate on enzyme hydrolysis.

    X-Gal

    X-gal (5-Bromo-4-chloro-3-indolyl-β-galactopyranoside) is the most widely used chromogenic substrate for β-galactosidase. Enzyme hydrolysis of the substrate yields a dark blue colored precipitate (maximum absorbance 615 nm).

    Red-β-D-Gal

    Red-β-D-Gal (5-Bromo-6-chloro-3-indolyl-β-D-galactopyranoside) is a chromogenic substrate for β-galactosidase. Similar to X-gal, but generates a red or magenta (λmax ~ 565 nm) colored precipitate on enzyme hydrolysis.

    Rose-β-D-Gal

    Rose-β-D-Gal (6-Chloro-3-indoxyl-β-D-galactopyranoside) is a chromogenic substrate for β-galactosidase. The product is similar to X-gal, but generates an intense pink colored precipitate (λmax ~ 540 nm) on enzymatic hydrolysis.

    Green-β-D-Gal

    Green-β-D-Gal (N-Methylindolyl-β-D-galactopyranoside) is a chromogenic substrate for β-galactosidase. Similar to X-Gal, but generates green colored precipitate on enzyme hydrolysis.