Ethidium Homodimer III (EthD-III), 1 mM in DMSO
A red fluorescent dead cell stain for bacteria and mammalian cells
Ethidium Homodimer III, also known as EthD-III, is a red fluorescent dead cell stain for bacteria and mammalian cells. It is a cell membrane-impermeant nucleic acid dye that stains only dead cells with damaged cell membranes. Ethidium Homodimer III was developed by Biotium as a superior alternative to Ethidium Homodimer I. The absorption and emission spectra are similar, but EthD-III is 45% brighter.
Ethidium Homodimer III is membrane impermeant and useful for selectively staining dead cells with damaged plasma membranes. It also is a component of several combination viability assay kits for detecting both live and dead cells in the same population, such as our Viability/Cytotoxicity Assay Kit for Animal Cells (Cat. No 30002) and our Viability/Cytotoxicity Assay Kit for Bacteria (Cat. No. 30027).
We also offer Ethidium Homodimer III in solid form (catalog no. 40050).
EthD-III is dead cell specific in all cell types, including mammalian cells, bacteria and yeast. See our Cellular Stains Table for more information on how our dyes stain various organisms.
- λEx/λEm (with DNA) = 522/593 nm* (EthD-III also has a large absorption peak at 279 nm)
- 1 mM solution in DMSO
- Store at 4°C and protect from light
- MW: ~1000