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Ethidium Homodimer III (EthD-III), 1 mM in DMSO

A red fluorescent dead cell stain for bacteria and mammalian cells

Product Attributes

Cellular localization

Nucleus & cytoplasm

For live or fixed cells

For live/intact cells

Assay type/options

Real-time imaging

Cell permeability

Membrane impermeant

Apoptosis/viability marker

Dead cell stain

Colors

Red

Size
Catalog #
price
Qty
200 uL
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Product Description

Ethidium Homodimer III, also known as EthD-III, is a red fluorescent dead cell stain for bacteria and mammalian cells. It is a cell membrane-impermeant nucleic acid dye that stains only dead cells with damaged cell membranes. Ethidium Homodimer III was developed by Biotium as a superior alternative to Ethidium Homodimer I. The absorption and emission spectra are similar, but EthD-III is 45% brighter.

Ethidium Homodimer III is membrane impermeant and useful for selectively staining dead cells with damaged plasma membranes. It also is a component of several combination viability assay kits for detecting both live and dead cells in the same population, such as our Viability/Cytotoxicity Assay Kit for Animal Cells (Cat. No 30002) and our Viability/Cytotoxicity Assay Kit for Bacteria (Cat. No. 30027). We also offer Ethidium Homodimer III in solid form (catalog no. 40050).

  • λExEm (with DNA) = 522/593 nm* (EthD-III also has a large absorption peak at 279 nm)
  • 1 mM solution in DMSO
  • Store at 4°C and protect from light
  • MW: ~1000

 

References

n/a

 

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