Our aminooxy reagents such as aminooxy-5(6)-FAM react with aldehydes or ketones to form a stable hydrazone compound under mild conditions. Similar to ARP (90073), aminooxy-5(6)-FAM can be used to label abasic sites in damaged DNA.
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CF® Dye & Biotin SE Protein Labeling Kits
This kit contains everything you need to label antibodies or other proteins with a fluorescent CF® dye succinimidyl ester (CF® dye SE) or biotin succinimidyl ester (biotin SE). Each kit contains reagents for three labeling reactions of up to 1 mg protein per reaction.
Anhydrous DMSO (dimethyl sulfoxide; methyl sulfoxide) is recommended for preparing stock solutions of AM ester dyes, reactive dyes and other related moisture-sensitive products.
Aniline, 10X in Acetate Buffer
Aniline, 10X in acetate buffer is designed to use as catalytic reagent for the optimal coupling of protein carbonyls with aminooxy compounds.
CF® Dye Aminooxy
Aminooxy dyes have an aminooxy reactive group, which can be used to label a target molecule with an aldehyde or ketone group. An aminooxy group readily reacts with an aldehyde or ketone group to form a stable linkage without the use of a reducing agent.
CF®DI dye hydrazide could be used to react with aldehyde or ketone groups from protein carbonylation. CF®DI dyes are designed to match for charge and molecular weight for 2D DIGE platform.