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Ethidium Homodimer III (EthD-III)

Ethidium Homodimer III was developed by Biotium as an alternative to Ethidium Homodimer I. It has absorption and emission spectra similar to those of Ethidium Homodimer I, but stains DNA 45% brighter.

Product Attributes

Cellular localization

Nucleus & cytoplasm

Assay type/options

Real-time imaging

Cell permeability

Membrane impermeant

Apoptosis/viability marker

Dead cell stain

Colors

Red

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Product Description

Ethidium Homodimer III was developed by Biotium as an alternative to Ethidium Homodimer I. It has absorption and emission spectra similar to those of Ethidium Homodimer I, but stains DNA 45% brighter.  The dye is membrane impermeant and useful for selectively staining dead cells with damaged plasma membranes; it also is a component of our Cell Viability/Cytotoxicity Assay Kit for detecting both live and dead cells in the same population (catalog no. 30002). We also offer Ethidium Homodimer III, 1 mM solution in DMSO (catalog no. 40051).

  • λExEm (with DNA) = 530/620 nm
  • Red solid soluble in DMSO, MeOH, or H2O
  • Store at 4°C and protect from light
  • MW: ~1000

 

References

1. Mol Cancer Res 6, 965 (2008).

2. J Pharmacol Exp Therap 332, 738 (2010).

 

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