Ethidium Homodimer III, also known as EthD-III, is a red fluorescent dead cell stain for bacteria and mammalian cells. It is a cell membrane-impermeant nucleic acid dye that stains only dead cells with damaged cell membranes. Ethidium Homodimer III was developed by Biotium as a superior alternative to Ethidium Homodimer I. The absorption and emission spectra are similar, but EthD-III is 45% brighter.
- Dead cell-specific DNA stain
- Can be used in mammalian, yeast, and bacteria cells
- 45% brighter than Ethidium Homodimer I
- Provided as a red solid
Ethidium Homodimer III is membrane impermeant and useful for selectively staining dead cells with damaged plasma membranes. It also is a component of several combination viability assay kits for detecting both live and dead cells in the same population, such as our Viability/Cytotoxicity Assay Kit for Animal Cells (Cat. No 30002) and our Viability/Cytotoxicity Assay Kit for Bacteria (Cat. No. 30027).
We also offer Ethidium Homodimer III, 1 mM solution in DMSO (catalog no. 40051).
EthD-III is dead cell specific in all cell types, including mammalian cells, bacteria and yeast. See our Cellular Stains Table for more information on how our dyes stain various organisms.
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2. J Pharmacol Exp Therap 332, 738 (2010).