Video from Essen Bioscience featuring NucView® 488 Caspase 3 Substrate
Cancer Cell Apoptosis Imaged by Incucyte® ZOOM with NucView® 488 Caspase-3 substrate
See NucView® 488 Caspase-3 Enzyme Substrate in action in this video provided by Essen Bioscience using their IncuCyte® ZOOM live cell imaging system. Cells from the HT-1080 human fibroscarcoma-derived cell line were treated with 150 nM camptothecin in the presence of 5 uM NucView® 488 Caspase-3 Substrate. The cells were imaged every 30 minutes for 24 hours, during which apoptosis can be observed by changes in cellular morphology and green fluorescence that is emitted upon DNA binding from the DNA binding NucView® 488 free dye released by the activity of caspase-3 upon the substrate.
Incucyte is a registered trademark of Essen Bioscience.
Featured webinar presented by Biotium and UVP
Fundamentals of Western Immunoblotting: Chemiluminescence and NIR Multiplex Imaging (49:44)
Fluorescent western blots are becoming an increasingly important technology for the detection of proteins using immunoassays. While chemiluminescence provides a high degree of sensitivity for reliable protein quantification in the lab, near-infrared (NIR) fluorescent Westerns allow researchers to rapidly probe for multiple proteins while also decreasing the time and labor required for blot development.
Webinar topics include:
• Advantages of Biotium’s CF® dyes for multiplex Westerns
• Fluorescent western blot development and imaging
• Detection of Western blots using CCD cameras compared to film
• UVP’s powerful and flexible instrumentation for Western blot imaging
How to use the Super HT PAP Pen
In this video, we demonstrate how to use Biotium’s Super HT PAP pen for preparing microscopy slides with hydrophobic barriers to prevent the waste of valuable reagents.
The SuperHT PAP Pen draws a water repellent circle around slide mounted tissue and prevents the waste of valuable reagents by keeping the liquid pooled in a single droplet. The regular size pen has a 4 mm tip and can be used for about 800 applications. The Mini SuperHT PAP Pen has a finer 2.5 mm tip, and can be used for about 400 applications. SuperHT Pap Pen barriers are insoluble in aqueous buffers, detergents, alcohol and acetone, but can be removed with xylene. Barriers are stable at temperatures up to 120°C.
View product here.