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Donkey Anti-Rabbit IgG (H+L), Highly Cross-Adsorbed

This product is prepared by labeling highly cross-adsorbed donkey anti-rabbit IgG (H+L) with our bright and photostable CF® dyes. To minimize cross-reactivity, the antibody has been adsorbed against  bovine, chicken, goat, guinea pig, Syrian hamster, horse, human, mouse, rat, and sheep serum.

Product Attributes

Antibody type

Secondary

Clonality

Polyclonal

Host species

Donkey

Reactivity (target)

Rabbit IgG

Species reactivity

Rabbit

Cross adsorption

Bovine, Chicken, Goat, Guinea pig, Horse, Human, Mouse, Rat, Sheep, Syrian hamster

Applications

Immunofluorescence, Immunohistology (formalin), Immunohistology (frozen), Western

Secondary antibody formats:
Liquid format:

  • CF® dye and biotin conjugates: 2 mg/mL in PBS, 50% glycerol, 2 mg/ml BSA, 0.05% sodium azide
  • HRP conjugates: 1 mg/mL in PBS, 50% glycerol, 15 mg/ml BSA
  • Alkaline phosphatase conjugates: 1 mg/mL in PBS, 40% glycerol, 5 mg/ml BSA, 0.05% sodium azide
  • R-PE and APC conjugates: 0.5 mg/mL in PBS, 2 mg/ml
    BSA, 0.05% sodium azide

Lyophilized format (after reconstitution):

  • CF® dye and biotin conjugates: 2 mg/mL in PBS, 15 mg/mL BSA, 20 mg/mL trehalose
  • HRP conjugates: 1 mg/mL PBS, 10 mg/mL trehalose
Conjugation
Size
Catalog #
price
Qty
50 uL
100 uL
250 uL
0.25 mL
500 uL
0.5 mL
1 mL
1 mg
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Product Description

This product is prepared by labeling highly cross-adsorbed donkey anti-rabbit IgG (H+L) with our bright and photostable CF® dyes. To minimize cross-reactivity, the antibody has been adsorbed against  bovine, chicken, goat, guinea pig, Syrian hamster, horse, human, mouse, rat, and sheep serum.

It’s available with the following CF® dyes and labels: CF®350, CF®405S, CF®430, CF®488A, CF®543, CF®555, CF®568, CF®594, CF®633, CF®640R, CF®647, CF®660R, CF®680, CF®680R, CF®750, CF®770, CF®790, CF®800, Alkaline Phosphatase, Biotin, and HRP.

Note: Conjugates of blue fluorescent dyes like CF®350 and CF®405S are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbed: Product & SKU Summary

ProductConjugationEx/EmSizeSKUPurchase
Alkaline Phosphatase Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedAlkaline PhosphataseN/A100 μL20467-100uLPurchase 20467-100uL
Alkaline Phosphatase Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedAlkaline PhosphataseN/A1 mL20467-1mLPurchase 20467-1mL
Biotin Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedBiotinN/A0.5 mL20191Purchase 20191
Biotin Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedBiotinN/A50 μL20191-1Purchase 20191-1
Biotin Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedBiotinN/A1 mg20191-1mgPurchase 20191-1mg
CF®350 Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF®350347/448 nm0.5 mL20351Purchase 20351
CF®350 Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF®350347/448 nm50 μL20351-1Purchase 20351-1
CF®350 Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF®350347/448 nm1 mg20351-1mgPurchase 20351-1mg
CF®405S Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF®405S404/431 nm1 mg20420-1mgPurchase 20420-1mg
CF®405S Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF®405S404/431 nm500 μL20420-500uLPurchase 20420-500uL
CF®405S Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF®405S404/431 nm50 μL20420-50uLPurchase 20420-50uL
CF®430 Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF®430426/498 nm1 mg20462-1mgPurchase 20462-1mg
CF®430 Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF®430426/498 nm500 μL20462-500uLPurchase 20462-500uL
CF®430 Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF®430426/498 nm50 μL20462-50uLPurchase 20462-50uL
CF®488A Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF®488A490/515 nm0.5 mL20015Purchase 20015
CF®488A Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF®488A490/515 nm50 μL20015-1Purchase 20015-1
CF®488A Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF®488A490/515 nm1 mg20015-1mgPurchase 20015-1mg
CF®543 Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF®543541/560 nm0.5 mL20308Purchase 20308
CF®543 Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF®543541/560 nm50 μL20308-1Purchase 20308-1
CF®543 Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF®543541/560 nm1 mg20308-1mgPurchase 20308-1mg
CF®555 Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF®555555/565 nm0.5 mL20038Purchase 20038
CF®555 Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF®555555/565 nm50 μL20038-1Purchase 20038-1
CF®555 Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF®555555/565 nm1 mg20038-1mgPurchase 20038-1mg
CF®568 Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF®568562/583 nm0.5 mL20098Purchase 20098
CF®568 Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF®568562/583 nm50 μL20098-1Purchase 20098-1
CF®568 Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF®568562/583 nm1 mg20098-1mgPurchase 20098-1mg
CF®594 Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF®594593/614 nm0.5 mL20152Purchase 20152
CF®594 Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF®594593/614 nm50 μL20152-1Purchase 20152-1
CF®594 Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF®594593/614 nm1 mg20152-1mgPurchase 20152-1mg
CF®633 Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF®633630/650 nm0.5 mL20125Purchase 20125
CF®633 Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF®633630/650 nm50 μL20125-1Purchase 20125-1
CF®633 Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF®633630/650 nm1 mg20125-1mgPurchase 20125-1mg
CF®640R Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF®640R642/662 nm0.5 mL20178Purchase 20178
CF®640R Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF®640R642/662 nm50 μL20178-1Purchase 20178-1
CF®640R Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF®640R642/662 nm1 mg20178-1mgPurchase 20178-1mg
CF®647 Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF®647650/665 nm0.5 mL20047Purchase 20047
CF®647 Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF®647650/665 nm50 μL20047-1Purchase 20047-1
CF®647 Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF®647650/665 nm1 mg20047-1mgPurchase 20047-1mg
CF®660R Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF®660R663/682 nm1 mg20389-1mgPurchase 20389-1mg
CF®660R Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF®660R663/682 nm0.5 mL20389-500uLPurchase 20389-500uL
CF®660R Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF®660R663/682 nm50 μL20389-50uLPurchase 20389-50uL
CF®680 Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF®680681/698 nm250 μL20418-250uLPurchase 20418-250uL
CF®680 Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF®680681/698 nm50 μL20418-50uLPurchase 20418-50uL
CF®680R Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF®680R680/701 nm0.5 mL20195Purchase 20195
CF®680R Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF®680R680/701 nm50 μL20195-1Purchase 20195-1
CF®750 Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF®750755/777 nm0.25 mL20298Purchase 20298
CF®750 Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF®750755/777 nm50 μL20298-1Purchase 20298-1
CF®770 Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF®770770/797 nm50 μL20484-50uLPurchase 20484-50uL
CF®770 Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF®770770/797 nm250 μL20484-250uLPurchase 20484-250uL
CF®790 Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF®790784/806 nm50 μL20344-50uLPurchase 20344-50uL
HRP Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedHRPN/A100 μL20405-100uLPurchase 20405-100uL
HRP Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedHRPN/A1 mg20405-1mgPurchase 20405-1mg
HRP Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedHRPN/A1 mL20405-1mLPurchase 20405-1mL

References

Download a list of CF® dye references.

 

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