Support

Quick links
Frequently asked questions - categories
Ask a question
Contact Us
+1

Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbed

This product is prepared by labeling highly cross-adsorbed donkey anti-rabbit IgG (H L) with our bright and photostable CF™ dyes. To minimize cross-reactivity, the antibody has been adsorbed against  bovine, chicken, goat, guinea pig, Syrian hamster, horse, human, mouse, rat, and sheep serum.

Placeholder

Secondary antibody formats:
Liquid format:

  • CF™ dye and biotin conjugates: 2 mg/mL in PBS, 50% glycerol, 2 mg/ml BSA, 0.05% sodium azide
  • HRP conjugates: 1 mg/mL in PBS, 50% glycerol, 15 mg/ml BSA
  • Alkaline phosphatase conjugates: 1 mg/mL in PBS, 40% glycerol, 5 mg/ml BSA, 0.05% sodium azide
  • R-PE and APC conjugates: 0.5 mg/mL in PBS, 2 mg/ml
    BSA, 0.05% sodium azide

Lyophilized format (after reconstitution):

  • CF™ dye and biotin conjugates: 2 mg/mL in PBS, 15 mg/mL BSA, 20 mg/mL trehalose
  • HRP conjugates: 1 mg/mL PBS, 10 mg/mL trehalose
Catalog #

20467-1mL, 20389-500uL, 20389-1mg, 20351-1mg, 20351-1, 20308-1mg, 20308-1, 20298-1, 20195-1, 20191-1mg, 20191-1, 20389-50uL, 20405-100uL, 20405-1mg, 20467-100uL, 20462-50uL, 20462-500uL, 20462-1mg, 20420-50uL, 20420-500uL, 20420-1mg, 20418-50uL, 20418-250uL, 20405-1mL, 20178-1mg, 20178-1, 20152-1mg, 20298, 20195, 20191, 20178, 20152, 20125, 20098, 20047, 20038, 20015, 20344-50uL, 20308, 20351, 20152-1, 20125-1mg, 20125-1, 20098-1mg, 20098-1, 20047-1mg, 20047-1, 20038-1mg, 20038-1, 20015-1mg, 20015-1, 20484-50uL, - 20484-250uLView allHide

Size

100 uL, 50 uL, 250 uL, 0.25 mL, 500 uL, 0.5 mL, 1 mL, 1 mg

Conjugation

Alkaline Phosphatase, Biotin, CF350, CF405S, CF430, CF488A, CF535ST, CF543, CF555, CF568, CF568ST, CF594, CF594ST, CF633, CF640R, CF647, CF647ST, CF660R, CF660ST, CF680, CF680R, CF680ST, CF750, CF750ST, CF770, CF790, HRP

Antibody type

Secondary

Species reactivity

Rabbit

Host species

Donkey

Reactivity (target)

Rabbit, Rabbit IgG

Cross adsorption

Bovine, Syrian hamster, Sheep, Rat, Mouse, Human, Horse, Guinea pig, Goat, Chicken

Applications

Immunofluorescence, Immunohistology (formalin), Western, Immunohistology (frozen)

Conjugation
Size
Catalog #
price
Qty
100 uL
50 uL
250 uL
0.25 mL
500 uL
0.5 mL
1 mL
1 mg
Clear selection
Add to wishlist
ADD TO CART
(Please select quantity above )

Wishlist updated! View wishlist

Shipping & Tax (CA only) not included in price. Learn more

Taxes: Tax is assessed in the state of California and is calculated during checkout based on your shipping information. Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions. Expedited shipment may be requested at time of checkout.

SPECIAL - Order 5 or more and receive 10% off.

Request a Bulk Quote

Product Description

This product is prepared by labeling highly cross-adsorbed donkey anti-rabbit IgG (H L) with our bright and photostable CF™ dyes. To minimize cross-reactivity, the antibody has been adsorbed against  bovine, chicken, goat, guinea pig, Syrian hamster, horse, human, mouse, rat, and sheep serum.

It’s available with the following CF™ dyes and labels: CF350, CF405S, CF430, CF488A, CF543, CF555, CF568, CF594, CF633, CF640R, CF647, CF660R, CF680, CF680R, CF750, , CF770, CF790, Alkaline Phosphatase, Biotin, and HRP.

Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbed: Product & SKU Summary

ProductConjugationEx/EmSizeSKUPurchase
Alkaline Phosphatase Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedAlkaline PhosphataseN/A100 μL20467-100uLPurchase 20467-100uL
Alkaline Phosphatase Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedAlkaline PhosphataseN/A1 mL20467-1mLPurchase 20467-1mL
Biotin Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedBiotinN/A0.5 mL20191Purchase 20191
Biotin Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedBiotinN/A50 μL20191-1Purchase 20191-1
Biotin Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedBiotinN/A1 mg20191-1mgPurchase 20191-1mg
CF350 Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF350347/448 nm0.5 mL20351Purchase 20351
CF350 Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF350347/448 nm50 μL20351-1Purchase 20351-1
CF350 Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF350347/448 nm1 mg20351-1mgPurchase 20351-1mg
CF405S Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF405S404/431 nm1 mg20420-1mgPurchase 20420-1mg
CF405S Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF405S404/431 nm500 μL20420-500uLPurchase 20420-500uL
CF405S Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF405S404/431 nm50 μL20420-50uLPurchase 20420-50uL
CF430 Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF430426/498 nm1 mg20462-1mgPurchase 20462-1mg
CF430 Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF430426/498 nm500 μL20462-500uLPurchase 20462-500uL
CF430 Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF430426/498 nm50 μL20462-50uLPurchase 20462-50uL
CF488A Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF488A490/515 nm0.5 mL20015Purchase 20015
CF488A Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF488A490/515 nm50 μL20015-1Purchase 20015-1
CF488A Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF488A490/515 nm1 mg20015-1mgPurchase 20015-1mg
CF543 Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF543541/560 nm0.5 mL20308Purchase 20308
CF543 Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF543541/560 nm50 μL20308-1Purchase 20308-1
CF543 Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF543541/560 nm1 mg20308-1mgPurchase 20308-1mg
CF555 Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF555555/565 nm0.5 mL20038Purchase 20038
CF555 Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF555555/565 nm50 μL20038-1Purchase 20038-1
CF555 Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF555555/565 nm1 mg20038-1mgPurchase 20038-1mg
CF568 Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF568562/583 nm0.5 mL20098Purchase 20098
CF568 Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF568562/583 nm50 μL20098-1Purchase 20098-1
CF568 Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF568562/583 nm1 mg20098-1mgPurchase 20098-1mg
CF594 Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF594593/614 nm0.5 mL20152Purchase 20152
CF594 Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF594593/614 nm50 μL20152-1Purchase 20152-1
CF594 Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF594593/614 nm1 mg20152-1mgPurchase 20152-1mg
CF633 Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF633630/650 nm0.5 mL20125Purchase 20125
CF633 Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF633630/650 nm50 μL20125-1Purchase 20125-1
CF633 Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF633630/650 nm1 mg20125-1mgPurchase 20125-1mg
CF640R Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF640R642/662 nm0.5 mL20178Purchase 20178
CF640R Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF640R642/662 nm50 μL20178-1Purchase 20178-1
CF640R Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF640R642/662 nm1 mg20178-1mgPurchase 20178-1mg
CF647 Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF647650/665 nm0.5 mL20047Purchase 20047
CF647 Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF647650/665 nm50 μL20047-1Purchase 20047-1
CF647 Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF647650/665 nm1 mg20047-1mgPurchase 20047-1mg
CF660R Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF660R663/682 nm1 mg20389-1mgPurchase 20389-1mg
CF660R Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF660R663/682 nm0.5 mL20389-500uLPurchase 20389-500uL
CF660R Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF660R663/682 nm50 μL20389-50uLPurchase 20389-50uL
CF680 Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF680681/698 nm250 μL20418-250uLPurchase 20418-250uL
CF680 Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF680681/698 nm50 μL20418-50uLPurchase 20418-50uL
CF680R Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF680R680/701 nm0.5 mL20195Purchase 20195
CF680R Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF680R680/701 nm50 μL20195-1Purchase 20195-1
CF750 Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF750755/777 nm0.25 mL20298Purchase 20298
CF750 Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF750755/777 nm50 μL20298-1Purchase 20298-1
CF770 Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF770770/797 nm50 μL20484-50uLPurchase 20484-50uL
CF770 Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF770770/797 nm250 μL20484-250uLPurchase 20484-250uL
CF790 Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedCF790784/806 nm50 μL20344-50uLPurchase 20344-50uL
HRP Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedHRPN/A100 μL20405-100uLPurchase 20405-100uL
HRP Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedHRPN/A1 mg20405-1mgPurchase 20405-1mg
HRP Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbedHRPN/A1 mL20405-1mLPurchase 20405-1mL

References

Download a list of CF dye references.

 

You may also like…

    EverBrite Mounting Medium

    Biotium's EverBrite™ mounting medium is a revolutionary antifade mounting medium optimally formulated for preserving fluorescence of our CF™ dyes and other fluorochromes. The mounting medium permits immediate imaging of slides and tissues after sealing edges of coverslips with nail polish or Biotium's CoverGrip™ Coverslip Sealant.

    CoverGrip Coverslip Sealant

    CoverGrip™ Coverslip Sealant is the first product specifically designed for sealing the edges of wet-mounted coverslips for fluorescence microscopy. Unlike nail polish, CoverGrip™ contains no ingredients that can leach into aqueous mounting medium and affect specimen fluorescence.

    TrueBlack Lipofuscin Autofluorescence Quencher

    TrueBlack™ Lipofuscin Autofluorescence Quencher, 20X in DMF, is a new reagent for quenching lipofuscin autofluorescence in tissue sections for immunofluorescence staining. It can also help reduce autofluorescence from other sources like extracellular matrix.

    RedDot2 far-red nuclear stain, 200X in DMSO

    RedDot™2 is a far-red cell membrane-impermeable nuclear dye similar to Draq7™, with excitation and emission at 665/695 nm. The dye is ideal for specifically staining the nuclei of dead cells with minimal cytoplasmic RNA staining.

    Phalloidin Conjugates

    CF™ dye phalloidin conjugates consist of the mushroom toxin phalloidin labeled with our superior bright and photostable CF™ fluorescent dyes. Phalloidin conjugates are useful for staining F-actin in cells.

    Tyramide Amplification Kit with HRP Goat Anti-Mouse and CF Dye/Biotin Tyramide

    Tyramide amplification, sometimes called Catalyzed Reporter Deposition (CARD), is an enzyme-mediated detection method that uses the catalytic activity of horseradish peroxidase (HRP) to generate high density labeling of a target protein or nucleic acid sequence in situ.

Support & Faq

Product shelf life, solubility, shipping and storage, and stability

Bioscience kits
The guaranteed shelf life from date of receipt for bioscience kits is listed on the product information sheet. Some kits have an expiration date printed on the kit box label, this is the guaranteed shelf life date calculated from the day that the product shipped from our facility. Kits often are functional for significantly longer than the guaranteed shelf life. If you have an older kit in storage that you wish to use, we recommend performing a small scale positive control experiment to confirm that the kit still works for your application before processing a large number of samples or precious samples.

Antibodies and other conjugates
The guaranteed shelf life from date of receipt for antibodies and conjugates is listed on the product information sheet. Antibodies and other conjugates often are functional for significantly longer than the guaranteed shelf life. If you have an older conjugate in storage that you wish to use, we recommend performing a small scale positive control experiment to confirm that the product still works for your application before processing a large number of samples or precious samples.

For lyophilized antibodies, we recommend reconstituting the antibody with glycerol and antimicrobial preservative like sodium azide for the longest shelf life (note that sodium azide is not compatible with HRP-conjugates).

Chemicals, dyes, and gel stains
Biotium guarantees the stability of chemicals, dyes, and gel stains for at least a year from the date you receive the product. However, the majority of these products are highly stable for many years, as long as they are stored as recommended. Storage conditions can be found on the product information sheet or product safety and data sheet, material safety data sheet, and on the product label. Fluorescent compounds should be protected from light for long term storage.

If you have a Biotium compound that has been in storage for longer than one year that you wish to use, we recommend performing a small scale positive control experiment to confirm that the compound still works for your application before processing a large number of samples or precious samples.

Expiration date based on date of manufacture (DOM)
If your institution requires you to document expiration date based on date of manufacture for reagents, please contact techsupport@biotium.com for assistance.

Chemical products with special stability considerations:

Esters

Ester compounds include the following:
• Succinimidyl esters (SE, also known as NHS esters), such as our amine-reactive dyes
• Acetoxymethyl esters (AM esters) such as our membrane-permeable ion indicator dyes
• Diacetate-modified dyes, like ViaFluor™ 405, CFDA, and CFDA-SE cell viability/cell proliferation dyes

Ester dyes are stable in solid form as long as they are protected from light and moisture. Esters are not stable in aqueous solution. Concentrated stock solutions should be prepared in anhydrous DMSO (see Biotium catalog no. 90082). Stock solutions in anhydrous DMSO can be stored desiccated at -20°C for one month or longer. Esters should be diluted in aqueous solution immediately before use. Succinimidyl esters (SE) should be dissolved in a solution that is free of amine-containing compounds like Tris, glycine, or protein, which will react with the SE functional group. AM esters and diacetate compounds should be dissolved in a solution that is free of serum, because serum could contain esterases that would hydrolyze the compound.

A note on CF™ dye succinimidyl ester stability
Succinimidyl esters are generally susceptible to hydrolysis, which can result in lower labeling efficiency. Heavily sulfonated dyes, such as the Alexa Fluor® dyes, DyLight® dyes and IRDyes® are particularly hygroscopic, worsening the hydrolysis problem. For example, the percent of active Alexa Fluor® 488 succinimidyl ester (SE) could be well below 50% by the time of application (according to the manufacturer’s product datasheet). In a number of Alexa Fluor® SE reactive dyes, the SE group is derived from an aromatic carboxylic acid, while in all of Biotium’s CF™ dyes the SE group is prepared from an aliphatic carboxylic acid. This structural difference reduces the susceptibility of CF™ dye SE reactive groups to hydrolysis, resulting in relatively stable reactive dyes with consistently higher labeling efficiency compared to other SE derivatives of other fluorescent dyes.

Maleimides, MTS and thiosulfate dyes
Like the succinimidyl ester dyes, these dyes are also susceptible to hydrolysis, although generally to a much lower degree. Thus, for long term storage, anhydrous DMSO is recommended for making stock solutions.

Other reactive dyes
Amines, aminooxy (also known as oxylamine), hydrazide, azide, alkyne, BCN, and tyramide reactive dyes, as well as dye free acids, are generally stable in aqueous solution when stored at -20°C for 6-12 months or longer, as long as no compounds are present that may react with the dye’s functional group. See the product information sheets for specific reactive dyes more information.

Coelenterazines and D-luciferin

Coelenterazines are stable in solid form when stored as recommended; they are not stable in aqueous solution. Concentrated coelenterazine stock solutions (typically 1-100 mg/mL) should be prepared in ethanol or methanol; do not use DMSO or DMF to dissolve coelenterazines, because these solvents will oxidize the compounds. Ethanol or methanol stocks of coelenterazine can be stored at -20°C or below for six months or longer; alcohol stocks may evaporate during storage, so use tightly sealing screw cap vials and wrap the vials with Parafilm for long term storage. Propylene glycol also can be used as a solvent to minimize evaporation. If the solvent evaporates, the coelenterazine will still be present in the vial, so note the volume in the vial prior to storage so that you can adjust the solvent volume to correct for evaporation if needed. Prepare working solutions in aqueous buffers immediately before use. Coelenterazines are stable for up to five hours in aqueous solution.

Aquaphile™ coelenterazines are water soluble formulations of coelenterazines. They are stable in solid form when stored as recommended. Aquaphile™ coelenterazines should be dissolved in aqueous solution immediately before use. They are stable for up to five hours in aqueous solution.

Note that coelenterazines are predominantly yellow solids, but may contain dark red or brown flecks. This does not affect product stability or performance. If your coelenterazine is uniformly brown, then it is oxidized and needs to be replaced.

D-luciferin is stable in solid form and as a concentrated stock solution when stored as recommended; it is not stable at dilute working concentrations in aqueous solution. Prepare concentrated D-luciferin stock solutions (typically 1-100 mg/mL) in water, and store in aliquots at -20°C or below for six months or longer. Prepare working solutions immediately before use.

Category: Mix-n-Stain™ Antibody Labeling Kits, DNA Quantitation Kits, NucView™ Caspase 3 Enzyme Substrates, Luciferase Assays, CF™ Dyes, Product shelf life, solubility, shipping and storage, and stability

← FAQs

Many of our solid compounds are packaged by lyophilization, in which case they usually do not appear as fluffy powders, but form a film or coating on the sides of the vial. Simply add the appropriate volume of the recommended solvent to the vial to make the desired concentration stock solution, and swirl or gently vortex to mix. Make sure the solvent comes in contact with the inside walls of the vial to fully recover the product.

Category: Product shelf life, solubility, shipping and storage, and stability

← FAQs

Most of our products are stable at room temperature for many days, but we recommend storage at 4°C or -20°C to prolong shelf life. In the case of many of our aqueous dye solutions, the compounds are very stable at room temperature, but we recommend cold storage to prevent the growth of mold or other microbes over time. Therefore, to save on shipping costs, products with recommended storage at 4°C or -20°C may ship at ambient temperature without affecting product performance. When you receive the product, place it under the recommended storage conditions.

Category: Mix-n-Stain™ Antibody Labeling Kits, DNA Quantitation Kits, NucView™ Caspase 3 Enzyme Substrates, Luciferase Assays, CF™ Dyes, Product shelf life, solubility, shipping and storage, and stability

← FAQs

← FAQs

Ask a question

Shipping/Shelf-life

Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions. Expedited shipment may be requested at time of checkout. Please note that products with recommended storage at 4°C or -20°C may ship at ambient temperature. This will not affect product performance. When you receive the product, place it under the recommended storage conditions.