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Donkey Anti-Rabbit IgG (H+L), Highly Cross-Adsorbed

Highly cross-adsorbed donkey anti-rabbit IgG (H L) secondary antibody labeled with our superior CF® Dyes.

Product Attributes

Antibody type




Host species


Antibody reactivity (target)

Rabbit IgG

Species reactivity


Cross adsorption

Bovine, Chicken, Goat, Guinea pig, Horse, Human, Mouse, Rat, Sheep, Syrian hamster


2 mg/mL, 1 mg/mL (HRP, AP conjugates)

Antibody/conjugate formulation

Liquid: PBS/50% glycerol/2 mg/mL BSA/0.05% azide, Lyophilized: PBS/15 mg/mL BSA/20 mg/mL trehalose after reconstitution, HRP conjugates: PBS/50% glycerol/15 mg/mL BSA, HRP conjugates (lyophilized): PBS/10 mg/mL trehalose after reconstitution

Secondary/tag antibody applications

ELISA, Flow cytometry, IHC, IF (cells or tissue sections), Western blot

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Catalog #
50 uL
100 uL
250 uL
0.25 mL
500 uL
0.5 mL
1 mL
1 mg
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Product Description

This is a highly cross-adsorbed donkey anti-rabbit IgG (H L) secondary antibody labeled with our bright and photostable CF® Dyes. To minimize cross-reactivity, the antibody has been adsorbed against bovine, chicken, goat, guinea pig, Syrian hamster, horse, human, mouse, rat, and sheep serum.

  • Highly cross-adsorbed for specific staining with minimal background
  • Available with 19 bright and photostable CF® Dyes
  • Alkaline phosphatase, biotin, and HRP conjugates also available
  • Suitable for western, immunofluorescence, and immunohistology in FFPE tissues

Note: Conjugates of blue fluorescent dyes like CF®350 are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Donkey Anti-Rabbit IgG (H+L), Highly Cross-Adsorbed

ConjugationEx/EmSizeCatalog No.Dye Features
CF®350347/448 nm50 uL (100 ug)20351-1CF®350 Features
0.5 mL (1 mg)20351
1 mg20351-1mg
CF®405S404/431 nm50 uL (100 ug)20420-50uLCF®405S Features
0.5 mL (1 mg)20420-500uL
1 mg20420-1mg
CF®430426/498 nm50 uL (100 ug)20462-50uLCF®430 Features
0.5 mL (1 mg)20462-500uL
1 mg20462-1mg
CF®488A490/515 nm50 uL (100 ug)20015-1CF®488A Features
0.5 mL (1 mg)20015
1 mg20015-1mg
CF®543541/560 nm50 uL (100 ug)20308-1CF®543 Features
0.5 mL (1 mg)20308
1 mg20308-1mg
CF®555555/565 nm50 uL (100 ug)20038-1CF®555 Features
0.5 mL (1 mg)20038
1 mg20038-1mg
CF®568562/583 nm50 uL (100 ug)20098-1CF®568 Features
0.5 mL (1 mg)20098
1 mg20098-1mg
CF®583R585/609 nm50 uL (100 ug)20895-50uLCF®583R Features
0.5 mL (1 mg)20895-500uL
CF®594593/614 nm50 uL (100 ug)20152-1CF®594 Features
0.5 mL (1 mg)20152
1 mg20152-1mg
CF®633630/650 nm50 uL (100 ug)20125-1CF®633 Features
0.5 mL (1 mg)20125
1 mg20125-1mg
CF®640R642/662 nm50 uL (100 ug)20178-1CF®640R Features
0.5 mL (1 mg)20178
1 mg20178-1mg
CF®647650/665 nm50 uL (100 ug)20047-1CF®647 Features
0.5 mL (1 mg)20047
1 mg20047-1mg
CF®660R663/682 nm50 uL (100 ug)20389-50uLCF®660R Features
0.5 mL (1 mg)20389-500uL
1 mg20389-1mg
CF®680681/698 nm50 uL (100 ug)20418-50uLCF®680 Features
0.25 mL (500 ug)20418-250uL
CF®680R680/701 nm50 uL (100 ug)20195-1CF®680R Features
0.25 mL (500 ug)20195
CF®750755/777 nm50 uL (100 ug)20298-1CF®750 Features
0.25 mL (500 ug)20298
CF®770770/797 nm50 uL (100 ug)20484-50uLCF®770 Features
0.25 mL (500 ug)20484-250uL
CF®790784/806 nm50 uL (100 ug)20344-50uLCF®790 Features
BiotinN/A50 uL (100 ug)20191-1
0.5 mL (1 mg)20191
1 mg20191-1mg
HRPN/A100 uL (100 ug)20405-100uL
1 mL (1 mg)20405-1mL
1 mg20405-1mg
APN/A100 uL (100 ug)20467-100uL
1 mL (1 mg)20467-1mL

View our full selection of Secondary Antibodies, or search our catalog using our Antibody Finder. Alternatively, you can view our secondary antibody product listings with catalog numbers.

CF® Dyes offer exceptional brightness and photostability. For more information see our CF® Dye technology page.

Storage and Handling

Liquid format: Store at -20°C, protected from light. Product is stable for at least 6 months from date of receipt when stored as recommended. Liquid format antibodies contain 50% glycerol and will not freeze at -20°C.

Lyophilized format: Store at -20°C, protected from light. Product is stable for at least 6 months from date of receipt when stored as recommended. Reconstitute antibodies in water using the indicated volumes below:

CF® Dye and biotin conjugates: add 0.5 mL dH2O

HRP or DNP conjugates: add 1 mL dH2O

Add the indicated volume of water directly to the vial containing the lyophilized antibody and mix gently to dissolve. Store reconstituted antibody at -20°C and protect from light. Aliquot to avoid repeated freeze/thaw cycles. Alternatively, an equal volume of glycerol can be mixed with the reconstituted antibody so that it will remain liquid at -20°C.

Optional: A preservative such as 0.05% sodium azide (final concentration) can be added to CF® Dye and biotin conjugates. Do not add sodium azide to HRP conjugates.

Note: Storage of the antibody for more than a day at final working dilution is not recommended.

CF is a registered trademark of Biotium, Inc.


Download a list of curated CF® Dye references.


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