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Donkey Anti-Mouse IgG (H+L), Highly Cross-Adsorbed (Min X Rat)

Highly cross-adsorbed donkey anti-mouse IgG (H L) secondary antibody labeled with our superior CF® Dyes and other labels.

Product Attributes

Antibody type

Secondary

Clonality

Polyclonal

Host species

Donkey

Antibody reactivity (target)

Mouse IgG

Species reactivity

Mouse

Cross adsorption

Bovine, Chicken, Goat, Guinea pig, Horse, Human, Rabbit, Rat, Sheep, Syrian hamster

Concentration

2 mg/mL, 1 mg/mL (HRP, AP conjugates)

Antibody/conjugate formulation

Liquid: PBS/50% glycerol/2 mg/mL BSA/0.05% azide, Lyophilized: PBS/15 mg/mL BSA/20 mg/mL trehalose after reconstitution, HRP conjugates: PBS/50% glycerol/15 mg/mL BSA, HRP conjugates (lyophilized): PBS/10 mg/mL trehalose after reconstitution

Secondary/tag antibody applications

ELISA, Flow cytometry, IHC, IF (cells or tissue sections), Western blot

Conjugation
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Catalog #
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50 uL
100 uL
0.25 mL
500 uL
0.5 mL
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1 mg
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Product Description

This is a highly cross-adsorbed donkey anti-mouse IgG (H L) secondary antibody labeled with our bright and photostable CF® Dyes and other labels. To minimize cross-reactivity, the antibody has been adsorbed against bovine, chicken, goat, guinea pig, Syrian hamster, horse, human, rabbit, rat and sheep serum proteins.

  • Highly cross-adsorbed for specific staining with minimal background
  • Available in 16 bright and photostable CF® Dyes
  • Biotin, AP, and HRP conjugates also available
  • Suitable for western, immunofluorescence, and immunohistology

Note: Conjugates of blue fluorescent dyes like CF®350 are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Donkey Anti-Mouse IgG (H+L), Highly Cross-Adsorbed (Min X Rat)

ConjugationEx/EmSizeCatalog No.Dye Features
CF®350347/448 nm50 uL (100 ug)20350-1CF®350 Features
0.5 mL (1 mg)20350
1 mg (lyophilized)20350-1mg
CF®430426/498 nm50 uL (100 ug)20461-50uLCF®430 Features
0.5 mL (1 mg)20461-500uL
1 mg (lyophilized)20461-1mg
CF®488A490/515 nm50 uL (100 ug)20014-1CF®488A Features
0.5 mL (1 mg)20014
1 mg (lyophilized)20014-1mg
CF®514516/548 nm50 uL (100 ug)20483-50uLCF®514 Features
0.5 mL (1 mg)20483-500uL
1 mg (lyophilized)20483-1mg
CF®543541/560 nm50 uL (100 ug)20305-1CF®543 Features
0.5 mL (1 mg)20305
1 mg (lyophilized)20305-1mg
CF®555555/565 nm50 uL (100 ug)20037-1CF®555 Features
0.5 mL (1 mg)20037
1 mg (lyophilized)20037-1mg
CF®568562/583 nm50 uL (100 ug)20105-1CF®568 Features
0.5 mL (1 mg)20105
1 mg (lyophilized)20105-1mg
CF®583R585/609 nm50 uL (100 ug)20894-50uLCF®583R Features
0.5 mL (1 mg)20894-500uL
CF®594593/614 nm50 uL (100 ug)20115-1CF®594 Features
0.5 mL (1 mg)20115
1 mg (lyophilized)20115-1mg
CF®633630/650 nm50 uL (100 ug)20124-1CF®633 Features
0.5 mL (1 mg)20124
1 mg (lyophilized)20124-1mg
CF®640R642/662 nm50 uL (100 ug)20177-1CF®640R Features
0.5 mL (1 mg)20177
1 mg (lyophilized)20177-1mg
CF®647650/665 nm50 uL (100 ug)20046-1CF®647 Features
0.5 mL (1 mg)20046
1 mg (lyophilized)20046-1mg
CF®660R663/682 nm50 uL (100 ug)20388-50uLCF®660R Features
0.5 mL (1 mg)20388-500uL
1 mg (lyophilized)20388-1mg
CF®680R680/701 nm50 uL (100 ug)20194-1CF®680R Features
0.25 mL (500 ug)20194
CF®790784/806 nm50 uL (100 ug)20363-50uLCF®790 Features
CF®800797/816 nm50 uL (100 ug)20835-50uLCF®800 Features
BiotinN/A50 uL (100 ug)20190-1
0.5 mL (1 mg)20190
1 mg (lyophilized)20190-1mg
HRPN/A100 uL (100 ug)20404-100uL
1 mL (1 mg)20404-1mL
1 mg (lyophilized)20404-1mg
APN/A100 uL (100 ug)20466-100uL
1 mL (1 mg)20466-1mL

View our full selection of bright and specific Secondary Antibodies, or search our catalog using our Antibody Finder. Alternatively, you can view our secondary antibody product listings with catalog numbers.

CF® Dyes offer exceptional brightness and photostability. For more information see our CF® Dye technology page.

Storage and Handling

Liquid format: Store at -20°C, protected from light. Product is stable for at least 6 months from date of receipt when stored as recommended. Liquid format antibodies contain 50% glycerol and will not freeze at -20°C.

Lyophilized format: Store at -20°C, protected from light. Product is stable for at least 6 months from date of receipt when stored as recommended. Reconstitute antibodies in water using the indicated volumes below:

CF® Dye and biotin conjugates: add 0.5 mL dH2O

HRP or DNP conjugates: add 1 mL dH2O

Add the indicated volume of water directly to the vial containing the lyophilized antibody and mix gently to dissolve. Store reconstituted antibody at -20°C and protect from light. Aliquot to avoid repeated freeze/thaw cycles. Alternatively, an equal volume of glycerol can be mixed with the reconstituted antibody so that it will remain liquid at -20°C.

Optional: A preservative such as 0.05% sodium azide (final concentration) can be added to CF® Dye and biotin conjugates. Do not add sodium azide to HRP conjugates.

Note: Storage of the antibody for more than a day at final working dilution is not recommended.

CF is a registered trademark of Biotium, Inc.

References

Download a list of curated CF® Dye references.

 

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