Support

Quick links
Frequently asked questions - categories
Ask a question
Contact Us
+1

Donkey Anti-Mouse IgG (H+L), highly cross-adsorbed

This product is prepared by labeling highly cross-adsorbed donkey anti-mouse IgG (H+L) with a selection of our bright and photostable CF™ dyes and other labels. To minimize cross-reactivity, the antibody has been adsorbed against bovine, chicken, goat, guinea pig, Syrian hamster, horse, human, rabbit, and sheep serum.

Placeholder

Secondary antibody formats:
Liquid format:

  • CF™ dye and biotin conjugates: 2 mg/mL in PBS, 50% glycerol, 2 mg/ml BSA, 0.05% sodium azide
  • HRP conjugates: 1 mg/mL in PBS, 50% glycerol, 15 mg/ml BSA
  • Alkaline phosphatase conjugates: 1 mg/mL in PBS, 40% glycerol, 5 mg/ml BSA, 0.05% sodium azide
  • R-PE and APC conjugates: 0.5 mg/mL in PBS, 2 mg/ml
    BSA, 0.05% sodium azide

Lyophilized format (after reconstitution):

  • CF™ dye and biotin conjugates: 2 mg/mL in PBS, 15 mg/mL BSA, 20 mg/mL trehalose
  • HRP conjugates: 1 mg/mL PBS, 10 mg/mL trehalose
Catalog #

20466-1mL, 20350-1, 20305-1mg, 20305-1, 20194-1, 20190-1mg, 20190-1, 20177-1mg, 20177-1, 20124-1mg, 20350-1mg, 20388-1mg, 20466-100uL, 20461-50uL, 20461-500uL, 20461-1mg, 20404-1mL, 20404-1mg, 20404-100uL, 20388-50uL, 20388-500uL, 20124-1, 20115-1mg, 20190, 20177, 20124, 20115, 20105, 20046, 20037, 20014, 20363-50uL, 20194, 20305, 20350, 20115-1, 20105-1mg, 20105-1, 20046-1mg, 20046-1, 20037-1mg, 20037-1, 20014-1mg, 20014-1, 20483-1mg, 20483-500uL, - 20483-50uLView allHide

Size

50 uL, 100 uL, 500 uL, 0.5 mL, 1 mL, 1 mg

Conjugation

Alkaline Phosphatase, Biotin, CF350, CF430, CF488A, CF514, CF543, CF555, CF568, CF594, CF633, CF640R, CF647, CF660R, CF680R, CF790, HRP

Antibody type

Secondary

Species reactivity

Mouse

Host species

Donkey

Reactivity (target)

Mouse, Mouse IgG

Cross adsorption

Bovine, Syrian hamster, Sheep, Rabbit, Human, Horse, Guinea pig, Goat, Chicken

Applications

Immunofluorescence, Immunohistology (formalin), Western, Immunohistology (frozen)

Conjugation
Size
Catalog #
price
Qty
50 uL
100 uL
500 uL
0.5 mL
1 mL
1 mg
Clear selection
Add to wishlist
ADD TO CART
(Please select quantity above )

Wishlist updated! View wishlist

Shipping & Tax (CA only) not included in price. Learn more

Taxes: Tax is assessed in the state of California and is calculated during checkout based on your shipping information. Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions. Expedited shipment may be requested at time of checkout.

SPECIAL - Order 5 or more and receive 10% off.

Request a Bulk Quote

Product Description

This product is prepared by labeling highly cross-adsorbed donkey anti-mouse IgG (H+L) with a selection of fluorescent CF™ dyes and other labels. To minimize cross-reactivity, the antibody has been adsorbed against bovine, chicken, goat, guinea pig, Syrian hamster, horse, human, rabbit, and sheep serum.

CF™ dyes offer exceptional brightness and photostability. See the CF™ Dye Selection Guide under Downloads for more information.

Note: Conjugates of blue fluorescent dyes like CF™350 are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Donkey Anti-Mouse IgG (H+L), highly cross-adsorbed: Product & SKU Summary

ProductConjugationEx/EmSizeSKUPurchase
Biotin Donkey Anti-Mouse IgG (H+L), highly cross-adsorbedBiotinN/A0.5 mL20190Purchase 20190
Biotin Donkey Anti-Mouse IgG (H+L), highly cross-adsorbedBiotinN/A50 μL20190-1Purchase 20190-1
Biotin Donkey Anti-Mouse IgG (H+L), highly cross-adsorbedBiotinN/A1 mg20190-1mgPurchase 20190-1mg
CF350 Donkey Anti-Mouse IgG (H+L), highly cross-adsorbedCF350347/448 nm0.5 mL20350Purchase 20350
CF350 Donkey Anti-Mouse IgG (H+L), highly cross-adsorbedCF350347/448 nm50 μL20350-1Purchase 20350-1
CF350 Donkey Anti-Mouse IgG (H+L), highly cross-adsorbedCF350347/448 nm1 mg20350-1mgPurchase 20350-1mg
CF430 Donkey Anti-Mouse IgG (H+L), highly cross-adsorbedCF430426/498 nm1 mg20461-1mgPurchase 20461-1mg
CF430 Donkey Anti-Mouse IgG (H+L), highly cross-adsorbedCF430426/498 nm500 μL20461-500uLPurchase 20461-500uL
CF430 Donkey Anti-Mouse IgG (H+L), highly cross-adsorbedCF430426/498 nm50 μL20461-50uLPurchase 20461-50uL
CF488A Donkey Anti-Mouse IgG (H+L), highly cross-adsorbedCF488A490/515 nm0.5 mL20014Purchase 20014
CF488A Donkey Anti-Mouse IgG (H+L), highly cross-adsorbedCF488A490/515 nm50 μL20014-1Purchase 20014-1
CF488A Donkey Anti-Mouse IgG (H+L), highly cross-adsorbedCF488A490/515 nm1 mg20014-1mgPurchase 20014-1mg
CF514 Donkey Anti-Mouse IgG (H+L), highly cross-adsorbedCF514516/548 nm1 mg20483-1mgPurchase 20483-1mg
CF514 Donkey Anti-Mouse IgG (H+L), highly cross-adsorbedCF514516/548 nm500 μL20483-500uLPurchase 20483-500uL
CF514 Donkey Anti-Mouse IgG (H+L), highly cross-adsorbedCF514516/548 nm50 μL20483-50uLPurchase 20483-50uL
CF543 Donkey Anti-Mouse IgG (H+L), highly cross-adsorbedCF543541/560 nm0.5 mL20305Purchase 20305
CF543 Donkey Anti-Mouse IgG (H+L), highly cross-adsorbedCF543541/560 nm50 μL20305-1Purchase 20305-1
CF543 Donkey Anti-Mouse IgG (H+L), highly cross-adsorbedCF543541/560 nm1 mg20305-1mgPurchase 20305-1mg
CF555 Donkey Anti-Mouse IgG (H+L), highly cross-adsorbedCF555555/565 nm0.5 mL20037Purchase 20037
CF555 Donkey Anti-Mouse IgG (H+L), highly cross-adsorbedCF555555/565 nm50 μL20037-1Purchase 20037-1
CF555 Donkey Anti-Mouse IgG (H+L), highly cross-adsorbedCF555555/565 nm1 mg20037-1mgPurchase 20037-1mg
CF568 Donkey Anti-Mouse IgG (H+L), highly cross-adsorbedCF568562/583 nm0.5 mL20105Purchase 20105
CF568 Donkey Anti-Mouse IgG (H+L), highly cross-adsorbedCF568562/583 nm50 μL20105-1Purchase 20105-1
CF568 Donkey Anti-Mouse IgG (H+L), highly cross-adsorbedCF568562/583 nm1 mg20105-1mgPurchase 20105-1mg
CF594 Donkey Anti-Mouse IgG (H+L), highly cross-adsorbedCF594593/614 nm0.5 mL20115Purchase 20115
CF594 Donkey Anti-Mouse IgG (H+L), highly cross-adsorbedCF594593/614 nm50 μL20115-1Purchase 20115-1
CF594 Donkey Anti-Mouse IgG (H+L), highly cross-adsorbedCF594593/614 nm1 mg20115-1mgPurchase 20115-1mg
CF633 Donkey Anti-Mouse IgG (H+L), highly cross-adsorbedCF633630/650 nm0.5 mL20124Purchase 20124
CF633 Donkey Anti-Mouse IgG (H+L), highly cross-adsorbedCF633630/650 nm50 μL20124-1Purchase 20124-1
CF633 Donkey Anti-Mouse IgG (H+L), highly cross-adsorbedCF633630/650 nm1 mg20124-1mgPurchase 20124-1mg
CF640R Donkey Anti-Mouse IgG (H+L), highly cross-adsorbedCF640R642/662 nm0.5 mL20177Purchase 20177
CF640R Donkey Anti-Mouse IgG (H+L), highly cross-adsorbedCF640R642/662 nm50 μL20177-1Purchase 20177-1
CF640R Donkey Anti-Mouse IgG (H+L), highly cross-adsorbedCF640R642/662 nm1 mg20177-1mgPurchase 20177-1mg
CF647 Donkey Anti-Mouse IgG (H+L), highly cross-adsorbedCF647650/665 nm0.5 mL20046Purchase 20046
CF647 Donkey Anti-Mouse IgG (H+L), highly cross-adsorbedCF647650/665 nm50 μL20046-1Purchase 20046-1
CF647 Donkey Anti-Mouse IgG (H+L), highly cross-adsorbedCF647650/665 nm1 mg20046-1mgPurchase 20046-1mg
CF660R Donkey Anti-Mouse IgG (H+L), highly cross-adsorbedCF660R663/682 nm1 mg20388-1mgPurchase 20388-1mg
CF660R Donkey Anti-Mouse IgG (H+L), highly cross-adsorbedCF660R663/682 nm0.5 mL20388-500uLPurchase 20388-500uL
CF660R Donkey Anti-Mouse IgG (H+L), highly cross-adsorbedCF660R663/682 nm50 μL20388-50uLPurchase 20388-50uL
CF680R Donkey Anti-Mouse IgG (H+L), highly cross-adsorbedCF680R680/701 nm0.5 mL20194Purchase 20194
CF680R Donkey Anti-Mouse IgG (H+L), highly cross-adsorbedCF680R680/701 nm50 μL20194-1Purchase 20194-1
CF790 Donkey Anti-Mouse IgG (H+L), highly cross-adsorbedCF790784/806 nm50 μL20363-50uLPurchase 20363-50uL
HRP Donkey Anti-Mouse IgG (H+L), highly cross-adsorbedHRPN/A100 μL20404-100uLPurchase 20404-100uL
HRP Donkey Anti-Mouse IgG (H+L), highly cross-adsorbedHRPN/A1 mg20404-1mgPurchase 20404-1mg
HRP Donkey Anti-Mouse IgG (H+L), highly cross-adsorbedHRPN/A1 mL20404-1mLPurchase 20404-1mL

References

Download a list of CF dye references.

 

You may also like…

Support & Faq

Product shelf life, solubility, shipping and storage, and stability

Bioscience kits
The guaranteed shelf life from date of receipt for bioscience kits is listed on the product information sheet. Some kits have an expiration date printed on the kit box label, this is the guaranteed shelf life date calculated from the day that the product shipped from our facility. Kits often are functional for significantly longer than the guaranteed shelf life. If you have an older kit in storage that you wish to use, we recommend performing a small scale positive control experiment to confirm that the kit still works for your application before processing a large number of samples or precious samples.

Antibodies and other conjugates
The guaranteed shelf life from date of receipt for antibodies and conjugates is listed on the product information sheet. Antibodies and other conjugates often are functional for significantly longer than the guaranteed shelf life. If you have an older conjugate in storage that you wish to use, we recommend performing a small scale positive control experiment to confirm that the product still works for your application before processing a large number of samples or precious samples.

For lyophilized antibodies, we recommend reconstituting the antibody with glycerol and antimicrobial preservative like sodium azide for the longest shelf life (note that sodium azide is not compatible with HRP-conjugates).

Chemicals, dyes, and gel stains
Biotium guarantees the stability of chemicals, dyes, and gel stains for at least a year from the date you receive the product. However, the majority of these products are highly stable for many years, as long as they are stored as recommended. Storage conditions can be found on the product information sheet or product safety and data sheet, material safety data sheet, and on the product label. Fluorescent compounds should be protected from light for long term storage.

If you have a Biotium compound that has been in storage for longer than one year that you wish to use, we recommend performing a small scale positive control experiment to confirm that the compound still works for your application before processing a large number of samples or precious samples.

Expiration date based on date of manufacture (DOM)
If your institution requires you to document expiration date based on date of manufacture for reagents, please contact techsupport@biotium.com for assistance.

Chemical products with special stability considerations:

Esters

Ester compounds include the following:
• Succinimidyl esters (SE, also known as NHS esters), such as our amine-reactive dyes
• Acetoxymethyl esters (AM esters) such as our membrane-permeable ion indicator dyes
• Diacetate-modified dyes, like ViaFluor™ 405, CFDA, and CFDA-SE cell viability/cell proliferation dyes

Ester dyes are stable in solid form as long as they are protected from light and moisture. Esters are not stable in aqueous solution. Concentrated stock solutions should be prepared in anhydrous DMSO (see Biotium catalog no. 90082). Stock solutions in anhydrous DMSO can be stored desiccated at -20°C for one month or longer. Esters should be diluted in aqueous solution immediately before use. Succinimidyl esters (SE) should be dissolved in a solution that is free of amine-containing compounds like Tris, glycine, or protein, which will react with the SE functional group. AM esters and diacetate compounds should be dissolved in a solution that is free of serum, because serum could contain esterases that would hydrolyze the compound.

A note on CF™ dye succinimidyl ester stability
Succinimidyl esters are generally susceptible to hydrolysis, which can result in lower labeling efficiency. Heavily sulfonated dyes, such as the Alexa Fluor® dyes, DyLight® dyes and IRDyes® are particularly hygroscopic, worsening the hydrolysis problem. For example, the percent of active Alexa Fluor® 488 succinimidyl ester (SE) could be well below 50% by the time of application (according to the manufacturer’s product datasheet). In a number of Alexa Fluor® SE reactive dyes, the SE group is derived from an aromatic carboxylic acid, while in all of Biotium’s CF™ dyes the SE group is prepared from an aliphatic carboxylic acid. This structural difference reduces the susceptibility of CF™ dye SE reactive groups to hydrolysis, resulting in relatively stable reactive dyes with consistently higher labeling efficiency compared to other SE derivatives of other fluorescent dyes.

Maleimides, MTS and thiosulfate dyes
Like the succinimidyl ester dyes, these dyes are also susceptible to hydrolysis, although generally to a much lower degree. Thus, for long term storage, anhydrous DMSO is recommended for making stock solutions.

Other reactive dyes
Amines, aminooxy (also known as oxylamine), hydrazide, azide, alkyne, BCN, and tyramide reactive dyes, as well as dye free acids, are generally stable in aqueous solution when stored at -20°C for 6-12 months or longer, as long as no compounds are present that may react with the dye’s functional group. See the product information sheets for specific reactive dyes more information.

Coelenterazines and D-luciferin

Coelenterazines are stable in solid form when stored as recommended; they are not stable in aqueous solution. Concentrated coelenterazine stock solutions (typically 1-100 mg/mL) should be prepared in ethanol or methanol; do not use DMSO or DMF to dissolve coelenterazines, because these solvents will oxidize the compounds. Ethanol or methanol stocks of coelenterazine can be stored at -20°C or below for six months or longer; alcohol stocks may evaporate during storage, so use tightly sealing screw cap vials and wrap the vials with Parafilm for long term storage. Propylene glycol also can be used as a solvent to minimize evaporation. If the solvent evaporates, the coelenterazine will still be present in the vial, so note the volume in the vial prior to storage so that you can adjust the solvent volume to correct for evaporation if needed. Prepare working solutions in aqueous buffers immediately before use. Coelenterazines are stable for up to five hours in aqueous solution.

Aquaphile™ coelenterazines are water soluble formulations of coelenterazines. They are stable in solid form when stored as recommended. Aquaphile™ coelenterazines should be dissolved in aqueous solution immediately before use. They are stable for up to five hours in aqueous solution.

Note that coelenterazines are predominantly yellow solids, but may contain dark red or brown flecks. This does not affect product stability or performance. If your coelenterazine is uniformly brown, then it is oxidized and needs to be replaced.

D-luciferin is stable in solid form and as a concentrated stock solution when stored as recommended; it is not stable at dilute working concentrations in aqueous solution. Prepare concentrated D-luciferin stock solutions (typically 1-100 mg/mL) in water, and store in aliquots at -20°C or below for six months or longer. Prepare working solutions immediately before use.

Category: Mix-n-Stain™ Antibody Labeling Kits, DNA Quantitation Kits, NucView™ Caspase 3 Enzyme Substrates, Luciferase Assays, CF™ Dyes, Product shelf life, solubility, shipping and storage, and stability

← FAQs

Many of our solid compounds are packaged by lyophilization, in which case they usually do not appear as fluffy powders, but form a film or coating on the sides of the vial. Simply add the appropriate volume of the recommended solvent to the vial to make the desired concentration stock solution, and swirl or gently vortex to mix. Make sure the solvent comes in contact with the inside walls of the vial to fully recover the product.

Category: Product shelf life, solubility, shipping and storage, and stability

← FAQs

Most of our products are stable at room temperature for many days, but we recommend storage at 4°C or -20°C to prolong shelf life. In the case of many of our aqueous dye solutions, the compounds are very stable at room temperature, but we recommend cold storage to prevent the growth of mold or other microbes over time. Therefore, to save on shipping costs, products with recommended storage at 4°C or -20°C may ship at ambient temperature without affecting product performance. When you receive the product, place it under the recommended storage conditions.

Category: Mix-n-Stain™ Antibody Labeling Kits, DNA Quantitation Kits, NucView™ Caspase 3 Enzyme Substrates, Luciferase Assays, CF™ Dyes, Product shelf life, solubility, shipping and storage, and stability

← FAQs

← FAQs

Ask a question

Shipping/Shelf-life

Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions. Expedited shipment may be requested at time of checkout. Please note that products with recommended storage at 4°C or -20°C may ship at ambient temperature. This will not affect product performance. When you receive the product, place it under the recommended storage conditions.