Our One-Step protein gel stains are low-cost, quick, convenient and easy-to-use for protein gel (SDS-PAGE) staining. Staining is quick: 5 to 60 min in a single step without fixation or washing. These stains have non-toxic formulations for safer handling and easy disposal. One-Step stains are certified under CCR Title 22 as non-toxic to the environment for drain disposal after a simple pH neutralization step. For more information download the Safety Report for One-Step Protein Gel Stains.
The One-Step stains are compatible with Edman-based sequencing and with mass spectrometry.
- 100% H2O-based formulation
- NO stinky acetic acid
- NO toxic methanol
- NO microwaving
- NO washing
- NO fixing
Download our Protein Gel Stains Flyer.
One-Step Blue® Protein Gel Stain
One-Step Blue® Protein Gel Stain is a ready-to-use visible blue protein gel staining solution. With no fixation or wash steps required, One-Step Blue® is a great choice for convenience and safety. One-Step Blue® can detect as little as approximately 10-20 ng of protein per band.
- One-step staining procedure, no fixation required
- Non-toxic formulation is safe for easy disposal
- Sensitive protein detection down to 10-20 ng
- Visualize blue bands, or image with a near-infrared fluorescence imager
Clear visible protein staining in just 1 hour, no destaining required
One-Step Blue® produces fast (5-60 min) protein staining in a single step without fixation or washing. Destaining is optional. One-Step Blue® has comparable sensitivity as the widely used Coomassie Blue, with a lower limit of detection around 10-20 ng depending on the detection method used. Note that staining intensity varies between proteins. The staining is fully compatible with mass spectrometry and Edman-based sequencing. Proteins can be detected by visible blue staining, or by near-infrared fluorescence. In a comparison experiment with Coomassie Brilliant Blue R-250 and Simply Blue™ SafeStain, One-Step Blue® demonstrated much clearer staining after 1 hour of destaining in water compared to other stains (Figure 2.).
See the simple one-step staining procedure in this quick video
One-Step Lumitein™ & One-Step Lumitein™ UV
One-Step Lumitein™ and One-Step Lumitein™ UV are ready-to-use luminescent red protein gel stains that feature a convenient one-step fixation procedure with excellent sensitivity. Both gel stains are non-toxic and offer sensitive detection down to 1-10 ng in as little as 5 minutes. Staining is fully compatible with mass spectrometry and Edman-based sequencing.
One-Step Lumitein™ serves as an alternative to SYPRO® Ruby and may be imaged using a UV transilluminator with EtBr or SYBR filter set, a blue light transilluminator, or a laser-based gel scanner.
One-Step Lumitein™ UV is optimized for use on UV transilluminators and serves as a replacement for Oriole™ and Flamingo™ Protein Gel Stains. In addition, One-Step Lumitein™ UV is an aqueous-based solution that does not contain hazardous solvents that cause gel shrinkage, unlike Oriole™ Fluorescent Gel Stain (Figure 4.)
- One-step staining procedure, no fixation required
- Non-toxic formulation is safe for easy disposal
- Sensitive protein detection down to 1-10 ng
- One-Step Lumitein™ as a replacement for SYPRO® Ruby
- One-Step Lumitein™ UV as a replacement for Oriole™ and Flamingo™ Protein Gel Stains
Ordering Information
| Product | Replacement for | Catalog number | Unit size | Product protocol | Safety report |
|---|---|---|---|---|---|
| One-Step Blue® | Coomassie Brilliant Blue stains | 21003-1L | 1L | PI-21003 | One-Step Gel Stains Safety Report |
| One-Step Lumitein™ | SYPRO® Ruby Protein Gel Stain | 21004-1L | 1L | PI-21004 | |
| 21004-4L | 4L | ||||
| One-Step Lumitein™ UV | Oriole™ and Flamingo™ Protein Gel Stains | 21005-1L | 1L | PI-21005 | |
| 21005-4L | 4L |
One-Step Blue is a registered trademark of Biotium.
Lumitein and its related technologies are covered by US and international patents.
SYPRO is a registered trademark of Molecular Probes, Inc.
Oriole and Flamingo are trademarks of Bio-Rad Laboratories.
SimplyBlue is a trademark of Thermo Fisher Scientific.
Associated products
One-Step Blue® Protein Gel Stain
One-Step Lumitein™ Protein Gel Stain
21004-1L, - 21004-4LView allHide
One-Step Lumitein™ UV Protein Gel Stain
21005-1L, - 21005-4LView allHide