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May 21, 2025

Biotium Launches Highly Sensitive, Convenient, and Affordable Luminescent Viability Assay for HTS

By: NeuralabAdmin

New Steady-ATP™ HTS Viability Assay Kit offers exceptional sensitivity for measuring cell viability in a convenient single-step assay mix.

FREMONT, CA, March 21, 2025 — Biotium, a leading provider of innovative fluorescent reagents and kits for life science research, proudly announces the release of the Steady-ATP™ HTS Viability Assay Kit, an improved alternative to Biotium’s ATP-Glo™ Bioluminometric Cell Viability Assay for assessing cell proliferation or cytotoxic potential of compounds. The Steady-ATP™ HTS Viability Assay Kit is a luciferase-based ATP cell viability assay with high sensitivity, wide dynamic range, and long-lasting signal for high-throughput luminescence detection in microplates.

The Steady-ATP™ HTS Viability Assay Kit is designed for precise and sensitive assessment of cell viability through ATP quantification, a key indicator of cellular metabolic activity. This assay leverages an engineered Chemostable Luciferase with enhanced chemostability and thermostability, as well as an optimized lysis/assay buffer system to deliver a stable and long-lasting luminescent signal. Boasting exceptional sensitivity capable of detecting as few as 16 cells per well in a 384-well plate, the assay also offers researchers a wide dynamic range and excellent linearity over 3-4 orders of magnitude.

Key Features of the Steady-ATP™ HTS Viability Assay Kit:

  • Exceptional Sensitivity: Detection limit as few as 16 cells per well.
  • Wide Dynamic Range: Linear over 3-4 orders of magnitude.
  • Stable Signal: Half-life of over 5 hours, ideal for HTS platforms.
  • Cost-Effective: Comparable performance to leading assays at a lower cost.
The detection limit of Steady-ATP™ HTS Viability Assay Kit is as few as 16 cells/well in a 384-well plate format. Serial dilutions of Jurkat cells were tested in a black solid 384-well plate with 50 uL total assay volume per well and 30-minute incubation time. Luminescence signal was read on a BMG CLARIOstar® plate reader. Values represent the mean ± S.D. of four replicates.
The Steady-ATP™ assay builds upon Biotium’s legacy of excellence in luciferase assays, offering researchers a reliable solution for assessing cell proliferation and cytotoxicity across diverse experimental conditions. It is compatible with high-throughput screening platforms, facilitating efficient and scalable testing protocols.

For more information about the Steady-ATP™ HTS Viability Assay Kit and other innovative products from Biotium, please visit the product page.  Biotium also offers a wide selection of novel cell viability assays and stains, as well as high-performance luciferase reporter gene assays.


About

Biotium is a leading life science reagent manufacturer and supplier devoted to providing high-quality and innovative fluorescent tools that fuel scientific discovery. Our collaborative team of experienced chemists and biologists, who are at the forefront of fluorescent dye design, apply chemistry-based principles toward producing solutions for unmet challenges in life science and medical research. Since its founding in 2001, Biotium has developed over 30 patented technologies that have been licensed out to leading life science technology companies worldwide.

Media Contact: 
marketing@biotium.com
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