New Steady-ATP™ HTS Viability Assay Kit offers exceptional sensitivity for measuring cell viability in a convenient single-step assay mix.
FREMONT, CA, March 21, 2025 — Biotium, a leading provider of innovative fluorescent reagents and kits for life science research, proudly announces the release of the Steady-ATP™ HTS Viability Assay Kit, an improved alternative to Biotium’s ATP-Glo™ Bioluminometric Cell Viability Assay for assessing cell proliferation or cytotoxic potential of compounds. The Steady-ATP™ HTS Viability Assay Kit is a luciferase-based ATP cell viability assay with high sensitivity, wide dynamic range, and long-lasting signal for high-throughput luminescence detection in microplates.
The Steady-ATP™ HTS Viability Assay Kit is designed for precise and sensitive assessment of cell viability through ATP quantification, a key indicator of cellular metabolic activity. This assay leverages an engineered Chemostable Luciferase with enhanced chemostability and thermostability, as well as an optimized lysis/assay buffer system to deliver a stable and long-lasting luminescent signal. Boasting exceptional sensitivity capable of detecting as few as 16 cells per well in a 384-well plate, the assay also offers researchers a wide dynamic range and excellent linearity over 3-4 orders of magnitude.
Key Features of the Steady-ATP™ HTS Viability Assay Kit:
- Exceptional Sensitivity: Detection limit as few as 16 cells per well.
- Wide Dynamic Range: Linear over 3-4 orders of magnitude.
- Stable Signal: Half-life of over 5 hours, ideal for HTS platforms.
- Cost-Effective: Comparable performance to leading assays at a lower cost.

The Steady-ATP™ assay builds upon Biotium’s legacy of excellence in luciferase assays, offering researchers a reliable solution for assessing cell proliferation and cytotoxicity across diverse experimental conditions. It is compatible with high-throughput screening platforms, facilitating efficient and scalable testing protocols.
For more information about the Steady-ATP™ HTS Viability Assay Kit and other innovative products from Biotium, please visit the product page. Biotium also offers a wide selection of novel cell viability assays and stains, as well as high-performance luciferase reporter gene assays.