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CF®514 Dye

Alternative green fluorescent dye

Longer wavelength green dye can be imaged with FITC settings
Can be separated from CF®488A by linear unmixing
Bright, highly water soluble, and insensitive to pH

Figure 1. Normalized absorption and emission spectra of CF®514 goat anti-mouse IgG conjugate in PBS.

CF®514 is a green fluorescent dye that can be imaged using the same settings as FITC. When imaged using instruments capable of spectral imaging, CF®514 can be distinguished from CF®488A using linear unmixing software. This can allow the use of more than four colors in multicolor imaging using standard 4 color optics.

See CF®514 product lines below. Additional conjugates or reactive dye forms may be added on request, contact techsupport@biotium.com to inquire.

CF®514 Technical Summary

  • Abs/Em maxima: 516/548 nm
  • Extinction coefficient: 105,000
  • Molecular weight: ~1216
  • Excitation laser line: 488 nm
  • Direct replacement for: Alexa Fluor® 514

CF is a registered trademark of Biotium, Inc; Alexa Fluor is a registered trademark of Thermo Fisher Scientific.

CF®488A Dye < Previous       CF® Dyes Overview      Next > CF®532 Dye

Goat Anti-Mouse IgG (H+L)

From: $63 Sizes: 50 uL, 100 uL, 200 uL, 500 uL, 0.5 mL, 1 mL, 1 mgCatalog #: 20464-1mL, 20306-1mg, 20306-1, 20197-1mg, 20197-1, 20183-1mg, 20183-1, 20180-1mg, 20180-1, 20140-1mg, 20140-1, 20120-1mg, 20120-1, 20110-1mg, 20352-1mL, 20352-200uL, 20365-500uL, 20464-100uL, 20411-100uL, 20411-0.5mL, 20408-50uL, 20408-500uL, 20408-1mg, 20400-1mL, 20400-1mg, 20400-100uL, 20386-50uL, 20386-500uL, 20386-1mg, 20365-50uL, 20110-1, 20100-1mg, 20100-1, 20183, 20180, 20140, 20120, 20110, 20100, 20080, 20070, 20054, 20050, 20040, 20030, 20010, 20378-50uL, 20197, 20306, 20080-1mg, 20080-1, 20070-1mg, 20070-1, 20054-1mg, 20054-1, 20050-1mg, 20050-1, 20040-1mg, 20040-1, 20030-1mg, 20030-1, 20010-1mg, 20010-1, - 20365-1mgView allHide

Goat Anti-Rabbit IgG (H+L)

From: $63 Sizes: 50 uL, 100 uL, 200uL, 500 uL, 0.5 mL, 1 mL, 1 mgCatalog #: 20465-1mL, 20353-1mL, 20309-1mg, 20309-1, 20202-1mg, 20202-1, 20185-1mg, 20185-1, 20181-1mg, 20181-1, 20141-1mg, 20141-1, 20122-1mg, 20122-1, 20112-1mg, 20353-200uL, 20366-1mg, 20465-100uL, 20412-500uL, 20412-100uL, 20409-50uL, 20409-500uL, 20409-1mg, 20402-1mL, 20402-1mg, 20402-100uL, 20387-50uL, 20387-500uL, 20387-1mg, 20366-50uL, 20366-500uL, 20112-1, 20102-1mg, 20202, 20185, 20181, 20141, 20122, 20112, 20102, 20082, 20073, 20055, 20053, 20043, 20033, 20012, 20309, 20012-1, 20102-1, 20082-1mg, 20082-1, 20073-1mg, 20073-1, 20055-1mg, 20055-1, 20053-1mg, 20053-1, 20043-1mg, 20043-1, 20033-1mg, 20033-1, 20012-1mg, - 20379-50uLView allHide

Donkey Anti-Mouse IgG (H+L), highly cross-adsorbed

From: $79 Sizes: 50 uL, 100 uL, 500 uL, 0.5 mL, 1 mL, 1 mgCatalog #: 20466-1mL, 20350-1, 20305-1mg, 20305-1, 20194-1, 20190-1mg, 20190-1, 20177-1mg, 20177-1, 20124-1mg, 20350-1mg, 20388-1mg, 20466-100uL, 20461-50uL, 20461-500uL, 20461-1mg, 20404-1mL, 20404-1mg, 20404-100uL, 20388-50uL, 20388-500uL, 20124-1, 20115-1mg, 20190, 20177, 20124, 20115, 20105, 20046, 20037, 20014, 20363-50uL, 20194, 20305, 20350, 20115-1, 20105-1mg, 20105-1, 20046-1mg, 20046-1, 20037-1mg, 20037-1, 20014-1mg, 20014-1, 20483-1mg, 20483-500uL, 20483-50uL, - 20835-50uLView allHide

Streptavidin Conjugates

From: $93 Sizes: 100 uL, 200 uL, 1 mL, 1 mgCatalog #: 29030, 29056, 29065, 29066, 29072, 29044-1mL, 29044-200uL, 29048-1mL, 29048-200uL, 29071-100uL, 29071-1mL, 29049, 29043, 29041, 29031, 29032, 29033, 29034, 29035, 29036, 29037, 29038, 29039, 29040, - 29081View allHide

CF Dye Tyramides

From: $263 Sizes: 0.5 mgCatalog #: 92170, 96022, 96021, 92199, 92198, 92197, 92196, 92195, 92194, 92175, 92174, 92173, 92172, 92171, 96052, - 96053View allHide

CF Dye Succinimidyl Ester

From: $147 Sizes: 0.25 umol, 1 umolCatalog #: 92137, 92106, 92107, 92108, 92109, 92110, 92111, 92112, 92117, 92123, 96011, 96014, 96016, 92105, 92104, 92139, 92142, 92150, 92127, 92130, 92131, 92132, 92133, 92134, 92135, 92155, 92103, - 92120View allHide

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Faq

CF® Dyes

See our CF® Dye Quick Reference Table for a list of dyes and summary of their features. Our CF® Dye Selection Guide has more detailed information on each CF® dye, and ordering information for our various CF® dye product lines. You can download CF® dye normalized absorption and emission data in Excel format.

Category: Mix-n-Stain™ Antibody Labeling Kits, CF® Dyes

← FAQs
CF® initially was an abbreviation for Cyanine-based Fluorescent dyes. These were the first patented CF® dyes based on cyanine dye structures. Since then, our CF® dye patent portfolio has expanded to include four different fluorescent dye core structures that cover the fluorescence spectrum from UV to NIR.

Category: CF® Dyes

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The exact chemical structures of CF® dyes are currently confidential but will be fully disclosed at a later stage when pending patents become granted. In general terms, the structure of a CF® dye may be divided into two parts: a) dye core structure (i.e. the aromatic ring skeleton that defines the dye’s color or absorption/emission wavelengths), and b) core structure-modifying elements. At present, CF® dyes bear the core structures of coumarin, pyrene, rhodamine or cyanine dyes. Blue fluorescent CF® dyes are based on coumarin or pyrene dye core structure, while green to near-IR CF® dyes are based on either cyanine or rhodamine dye core structures. Core structure-modifying elements refer to various chemical attachments to the core structure and are a key aspect of the CF® dye invention that makes CF® dyes superior to other commercial dyes.

Category: CF® Dyes

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