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CF™514 Dye

CF™514 is a green fluorescent dye that can be imaged using the same settings as CF™488A or FITC. When imaged using instruments capable of spectral imaging, CF™514 can be distinguished from CF™488A using linear unmixing software.

A full selection of reactive dyes, secondary antibodies, antibody labeling kits, and other bioconjugates including phalloidins, Annexin V and α-bungarotoxin are available for CF™ dyes.

View products associated with this technology…

CF™514 conjugates, labeling kits, and reactive dyes

 

Technical Summary

  • Abs/Em Maxima: 516/548 nm
  • Extinction coefficient: 105,000
  • Molecular weight: ~1216
  • Excitation laser line: 488 nm
  • Direct replacement for: Alexa Fluor® 514
Figure 1: Absorption and emission spectra of CF514 goat anti-mouse conjugate in PBS.
Figure 1: Absorption and emission spectra of CF514 goat anti-mouse conjugate in PBS.
Figure 2. HeLa cells stained with mouse anti-tubulin antibody followed by CF514 goat anti-mouse conjugate.
Figure 2. HeLa cells stained with mouse anti-tubulin antibody followed by CF514 goat anti-mouse conjugate.