CF®620R Dye

CF®620R dye is a far-red rhodamine-based fluorescent dye. The dye is highly fluorescent and extremely photostable. With its absorption and emission maxima centered at 620 nm and 643 nm, respectively, the dye may be used as an excellent energy acceptor in fluorescence resonance energy transfer (FRET) when paired with R-PE, or used in multi-color detection applications with instruments that match the unique spectral profiles of the dye for maximal signal. The exceptional water solubility of the dye facilitates bioconjugation and better preserves biological specificity of the conjugates.

See products available with CF®620R. Additional reactive dyes or conjugates may be added on request, contact techsupport@biotium.com to inquire.

For bright and photostable far-red dyes for microscopy or flow cytometry, see our CF®633 and CF®640R dyes.

Figure 1. Normalized absorption and emission of CF®620R free acid in PBS.

CF®620R Technical Summary

Abs/Em maxima: 620/643 nm
Extinction coefficient: 115,000
Molecular weight: ~738
Laser line: 633 nm or 635 nm
Spectrally similar to: LightCycler® Red 640 (LC640)

Helpful Tools & Resources

Spectra Viewer

Visualize and export the fluorescent dye spectrum of any CF® Dye or other dyes from Biotium.

Download Our CF® Dye eBook

Learn the history of fluorophores in life science research as well as the development and advantages of CF® Dyes.

CF® Dye Selection Tool

Check out our handy fluorophore selection tool to help choose the best CF® Dye for your next experiment.

CF® Dye Reference Guide

Download our single page reference guide for all CF® Dye fluorophores. Includes recommended channels, applications, relative brightness, and photostability.