Menu
close
biotium logo
Antibody Finder
((0 ITEMS) ITEMS)
$0
Menu
close
Hero Image
Fluorescent CF® Dyes CF®532 Dye

CF®532 Dye

Bright green fluorescent dye for the 532 nm laser
  • Designed for the 532 nm laser
  • Brighter than Alexa Fluor® 532
  • Water soluble & pH-insensitive
CF® Dye Spectrum:
CF®350 Dye
CF®350 is a blue fluorescent dye
CF®405S Dye
Improved brightness & photostability for the 405 nm laser line
CF®405M Dye
Improved brightness & photostability for the 405 nm laser line
CF®430 & CF®440 Dyes
Photostable 405 nm-excitable dyes with green fluorescence
CF®405L Dye
A 405 nm-excitable dye with green fluorescence emission
CF®450 Dye
A spectrally unique, 405 nm-excitable green dye
CF®488A Dye
A superior green fluorescent dye
CF®503R Dye
A spectrally unique green dye ideal for spectral flow cytometry
CF®514 Dye
Alternative green fluorescent dye
CF®532 Dye
A bright green fluorescent dye for the 532 nm laser
CF®543 Dye
An orange fluorescent dye ideal for the 543 nm laser
CF®535ST Dye
A red fluorescent dye designed for STORM super-resolution imaging
CF®550R Dye
A spectrally unique orange/red dye ideal for spectral flow cytometry
CF®555 Dye
A bright and photostable orange-red dye
CF®568 Dye
Outshines Alexa Fluor®568
CF®570
Bright red fluorescent dye
CF®583 & CF®583R Dyes
Bright red fluorescent dyes
CF®594 Dye
Truly the brightest deep red dye
CF®597R Dye
Unique green-excited dye for STORM
RPE-Astral™616
Novel long Stokes shift tandem dye for flow cytometry multiplexing
CF®620R Dye
A bright and photostable far-red dye
CF®633 Dye
The best dye for 633/635 laser lines
CF®640R Dye
A highly photostable far-red dye
CF®647 Dye
A highly fluorescent far-red dye
CF®660C & CF®660R
Superior alternatives to Alexa Fluor® 660
CF®680 & CF®680R
Two outstanding 680 nm-excitable dyes
CF®700 Dye
Brighter alternative to Alexa Fluor® 700
CF®710 Dye
Near-infrared CF® Dye with exceptional photostability
CF®725 Dye
Near-infrared CF® Dye with exceptional photostability
CF®740 Dye
Near-infrared CF® Dye with exceptional photostability
CF®750 Dye
Near-infrared CF® Dye
CF®770 Dye
Near-infrared CF® Dye
CF®790 Dye
Near-infrared CF® Dye
APC-Astral™813
Novel long Stokes shift tandem dye for flow cytometry multiplexing
CF®800 Dye
Near-infrared CF® Dye
CF®820 Dye
Near-infrared CF® Dye
CF®850 Dye
Near-infrared CF® Dye
CF®870 Dye
Near-infrared CF® Dye

CF®532 is a green fluorescent dye with absorption/emission at 527/558 nm (Figure 1). Developed by Biotium, it’s a new rhodamine dye that’s bright, photostable, and highly water-soluble. CF®532 antibody conjugates are exceptionally soluble and brighter than similar dyes like Alexa Fluor® 532 (Figures 2, 3).

See products available with CF®532. Additional conjugates or reactive dyes may be added upon request; contact techsupport@biotium.com to inquire.

Figure 1. Normalized absorption and emission spectra of CF®532 goat anti-mouse conjugate in PBS.

CF®532 Technical Summary

  • Abs/Em maxima: 527/558 nm
  • Extinction coefficient: 96,000
  • Molecular weight: ~685
  • Laser line: 532 nm
  • Direct replacement for: Alexa Fluor® 532, Atto 532
Figure 2. CF®532 generates brighter fluorescent conjugates than Alexa Fluor® 532. Relative fluorescence of goat anti-mouse conjugates with varying degrees of la... See More
Figure 3. CF®532 gives brighter staining than Alexa Fluor® 532. Jurkat cells were stained with mouse anti-CD3 antibody, followed by goat anti-mouse conjugates w... See More

Helpful Tools & Resources

Visualize and export the fluorescent dye spectrum of any CF® Dye or other dyes from Biotium.
Learn the history of fluorophores in life science research as well as the development and advantages of CF® Dyes.
Check out our handy fluorophore selection tool to help choose the best CF® Dye for your next experiment.
Download our single page reference guide for all CF® Dye fluorophores. Includes recommended channels, applications, relative brightness, and photostability.
Previous
Previous
CF®514 Dye
Next
Next
CF®543 Dye

CF is a registered trademark of Biotium, Inc. Alexa Fluor is a registered trademark of Thermo Fisher Scientific. FACSCalibur is a trademark of BD Biosciences.

New Products

Antibodies, Conjugates, & Labeling
Cellular Stains & Assays
Molecular Biology
Protein Detection & Analysis
Enzyme Substrates & Assays
Reagents & Accessories by Research Area
Reagents & Accessories by Application

Content #1

Content #1

Content #1

Content #2

Content #3