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CF®680 & CF®680R

Outstanding 680 nm excitable dyes

Compatible with LI-COR® Odyssey® for NIR WB
Validated in STORM & STED super-resolution microscopy
CF®680R has unrivaled photostability
Figure 1. Normalized absorption and emission of CF®680 (solid lines) and CF®680R (dashed lines) goat anti-mouse conjugates in PBS.
Figure 2. CF®680 is brighter than IRDye® 680LT for near-infrared western blot. Two-fold dilutions of HeLa cell lysate (from 2 ug to 0.125 ug, lanes 1-5) were transferred to PVDF membranes. Blots were probed with mouse anti-tubulin and rabbit anti-COX IV antibodies, followed by IR®680LT or CF®680 anti-rabbit (red) and IRDye® 800CW or CF®770 anti-mouse (green). Membranes were scanned on a LI-COR® Odyssey® NIR imaging system. Quantitation of bands showed approximately 50% brighter signal with CF® dyes compared to IRDye®. M: molecular weight marker.

CF®680 and CF®680R are two outstanding near-IR dyes excited at 680 nm with emission near 700 nm. The dyes each have unique properties suited to different applications.

CF®680 is a highly water-soluble cyanine-based dye with a molecular weight of ~3000. This dye is excellent for labeling antibodies, producing the brightest fluorescence for near-infrared western blotting (Figure 2). With its long wavelength emission, CF®680 is also suitable for near-infrared small animal imaging. However, because of its relatively large molecular size, CF®680 is not recommended for labeling nucleic acids or small biomolecules, for which our CF®680R is better suited. CF®680 is validated in multicolor STORM and 3D super-resolution imaging (learn more about CF® dyes for super-resolution imaging).

CF®680R is a novel rhodamine-based dye with a relatively small molecular weight of ~912. The dye is highly fluorescent and, more importantly, extremely photostable (Figure 3). Rhodamine dyes are traditionally known to be bright and photostable. However, it has been a synthetic challenge to make functional rhodamine dyes with long wavelengths and high water solubility necessary for bioconjugation. Scientists at Biotium have overcome the challenge and invented a new way to make highly water soluble, bright and photostable rhodamine dyes with wavelengths ranging from red to near-IR. These dyes are excellent for labeling proteins, nucleic acids and small bio-molecules. They are ideal for confocal microscopy, single molecule imaging and other applications that demand both brightness and photostability. CF®680R is validated in STED super-resolution imaging and single molecule spectroscopy (see more CF® dyes for super-resolution microscopy).

CF™680 Technical Summary

  • Abs/Em maxima: 681/698 nm
  • Extinction coefficient: 210,000
  • Molecular weight: ~3241
  • Laser line: 680 or 685 nm
  • Replaces: Alexa Fluor® 680, Cy®5.5, DyLight®680, IRDye®680LT

CF™680R Technical Summary

  • Abs/Em maxima: 680/701 nm
  • Extinction coefficient: 140,000
  • Molecular weight: ~912
  • Laser line: 680 or 685 nm
  • Replaces: Alexa Fluor® 680, Cy®5.5, DyLight®680, IRDye®680LT

Figure 3. CF®680R is highly photostable. Jurkat cells were stained with mouse anti-CD3 antibody and goat anti-mouse conjugates of the dyes shown. Cells were exposed to continuous mercury arc lamp excitation with a Cy®5 filter set. Images were captured every 10 seconds for 5 minutes; mean fluorescence was normalized to time 0.
Figure 4. Comparison of microtubule imaging using conventional wide-field microscopy with STORM using CF®680 dye conjugate. Images courtesy of Sam Kenny and Professor Ke Xu, College of Chemistry, University of California, Berkeley.

CF is a registered trademark of Biotium, Inc. Alexa Fluor is a registered trademark of Thermo Fisher Scientific; Cy Dye is a registered trademark of GE Healthcare; IRDye and Odyssey are registered trademarks of LI-COR Biosciences.

CF®660C & CF®660R Dyes < Previous           CF® Dyes Overview           Next > CF®750, CF®770, CF®790 & CF®800 Dyes

Bioconjugates

Lectins, proteins, nucleotides, toxins, and other probes conjugated to our bright & photostable CF® dyes and other labels.

Primary Antibodies

Growing collection of more than 1000 monoclonal antibodies validated in IHC and other applications. Available with bright and photostable CF® dyes, R-PE, APC, PerCP, HRP, AP, biotin, or purified. Affordable 100 uL trial sizes.

CF® Dye Labeled & Biotinylated Secondary Antibodies

Many host/target species and cross-absorption options, featuring a wide spectrum of our bright & photostable CF® dyes or biotin.

CF® Dye Single Label Conjugates for STORM

Secondary antibody conjugates of our STORM-compatible CF® dyes, with an average degree of labeling (DOL) of one, specifically designed for super-resolution imaging by STORM.

Mix-n-Stain™ Antibody Labeling Kits

Mix-n-Stain™ kits use revolutionary technology that dramatically simplifies antibody labeling and requires no purification. The labeling is covalent and stable. Available with your choice of 24 CF® dyes, biotin, FITC, fluorescent proteins, enzymes, or haptens.

CF® Dye Protein Labeling Kits

Everything you need to label and purify antibodies or other proteins. Choose from 19 bright & photostable CF® dyes or biotin.

Vivobrite™ Rapid Antibody Labeling Kits for Small Animal Imaging

Everything you need to label and purify antibodies for near-infrared small animal imaging. Featuring our exceptional NIR CF® dyes.

Reactive CF® Dyes

Our bright, photostable, highly water soluble CF® dyes with a wide selection of colors and functional groups.

Additional conjugates or reactive dye forms may be added on request, contact techsupport@biotium.com to inquire.

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Faq

CF® Dyes

See our CF® Dye Quick Reference Table for a list of dyes and summary of their features. Our CF® Dye Selection Guide has more detailed information on each CF® dye, and ordering information for our various CF® dye product lines. You can download CF® dye normalized absorption and emission data in Excel format.

Category: Mix-n-Stain™ Antibody Labeling Kits, CF® Dyes

← FAQs
CF® initially was an abbreviation for Cyanine-based Fluorescent dyes. These were the first patented CF® dyes based on cyanine dye structures. Since then, our CF® dye patent portfolio has expanded to include four different fluorescent dye core structures that cover the fluorescence spectrum from UV to NIR.

Category: CF® Dyes

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The exact chemical structures of CF® dyes are currently confidential but will be fully disclosed at a later stage when pending patents become granted. In general terms, the structure of a CF® dye may be divided into two parts: a) dye core structure (i.e. the aromatic ring skeleton that defines the dye’s color or absorption/emission wavelengths), and b) core structure-modifying elements. At present, CF® dyes bear the core structures of coumarin, pyrene, rhodamine or cyanine dyes. Blue fluorescent CF® dyes are based on coumarin or pyrene dye core structure, while green to near-IR CF® dyes are based on either cyanine or rhodamine dye core structures. Core structure-modifying elements refer to various chemical attachments to the core structure and are a key aspect of the CF® dye invention that makes CF® dyes superior to other commercial dyes.

Category: CF® Dyes

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