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CF®405S Dye

Outshines Alexa Fluor® 405

Brighter conjugates compared to Alexa Fluor® 405
Better match for common instruments
Validated for SIM super-resolution imaging
CF® Dye Spectrum:
CF®350 Dye

CF®350 is a blue fluorescent dye

300400500600700800900Wavelength (nm)CF350
Technical Summary

Abs/Em Maxima: 347/448 nm
Extinction coefficient: 18,000
Molecular weight: ~ 496
Excitation source: UV
Replaces: Alexa Fluor® 350, AMCA,
DyLight® 350

Features

Brighter and more photostable than AMCA
Direct replacement for Alexa Fluor® 350
Highly water soluble and pH-insensitive

CF®405S Dye

Improved brightness & photostability for the 405 nm laser line

300400500600700800900Wavelength (nm)CF405S
Technical Summary

Abs/Em Maxima: 404/431 nm
Extinction coefficient: 33,000
Molecular weight: ~ 1,169
Excitation laser line: 405 nm
Replaces: Alexa Fluor® 405, Cascade Blue®,
DyLight® 405

Features

Brighter than Alexa Flour®  408

CF®405M Dye

Improved brightness & photostability for the 405 nm laser line

300400500600700800900Wavelength (nm)CF405M
Technical Summary

Abs/Em Maxima: 408/452 nm
Extinction coefficient: 41,000
Molecular weight: ~ 503
Excitation laser line: 405 nm
Replaces: Pacific Blue®, BD Horizon™ V450

Features

More photostable than Pacific Blue®, with less spill-over in the green channel
An excellent choice for super-resolution imaging by SIM

CF®405L Dye

A 405 nm-excitable dye with orange fluorescence emission

300400500600700800900Wavelength (nm)CF405L
Technical Summary

Abs/Em Maxima: 395/545 nm
Extinction coefficient: 24,000
Molecular weight: ~1573
Excitation laser line: 405 nm
Replaces: Pacific Orange®

Features

Expands potential detection channels for the 405 nm laser line

CF®430 & CF®440 Dyes

Photostable 405 nm-excitable dyes with green fluorescence

300400500600700800900Wavelength (nm)CF430CF440
Technical Summary

CF®430
Abs/Em Maxima: 426/498 nm
Extinction coefficient: 40,000
Molecular weight: ~429
Excitation laser line: 405 nm
Replaces: Pacific Green®, BD Horizon™ V500,
Krome Orange™

CF®440
Abs/Em Maxima: 440/515 nm
Extinction coefficient: 40,000
Molecular weight: ~716
Excitation laser line: 405 nm
Replaces: Alexa Fluor® 430

Features

Photostable dyes suitable for microscopy
Perfect match for the CFP filter set
Suitable for flow cytometry in the AmCyan channel
Highly water soluble and pH-insensitive

CF®450 Dye

A spectrally unique, 405 nm-excitable green dye.

300400500600700800900Wavelength (nm)CF450
Technical Summary

Abs/Em Maxima: 450/538 nm
Extinction coefficient: 40,000
Molecular weight: ~689
Excitation laser line: 405 nm

Features

A spectrally unique, 405 nm-excitable green dye.

CF®488A Dye

A superior green fluorescent dye

300400500600700800900Wavelength (nm)CF488A
Technical Summary

Abs/Em Maxima: 490/515 nm
Extinction coefficient: 70,000
Molecular weight: ~914
Excitation laser line: 488 nm
Replaces: Alexa Fluor® 488, DyLight® 488,
fluorescein (aka FITC, FAM), Cy®2

Features

Minimally charged, for less non-specific binding than Alexa Fluor® 488
Narrower emission spectrum for less bleed to red
Very photostable
Compatible with super-resolution imaging by TIRF
Highly water soluble and pH-insensitive

CF®503R Dye

A unique green dye or multispectral detection or FRET

300400500600700800900Wavelength (nm)CF503R
Technical Summary

Abs/Em maxima: 503/532 nm
Extinction coefficient: 90,000
Molecular weight: ~1100
Excitation laser line: 488 nm
Direct replacement for: ATTO 488

Features
CF®514 Dye

Alternative green fluorescent dye

300400500600700800900Wavelength (nm)CF514
Technical Summary

Abs/Em Maxima: 516/548 nm
Extinction coefficient: 105,000
Molecular weight: ~1216
Excitation laser line: 488 nm
Replaces: Alexa Fluor® 514

Features

Image using the same settings as FITC or CF®488A
Can be distinguished from CF®488A in the same specimen by spectral imaging and linear unmixing

CF®532 Dye

A bright orange fluorescent dye for the 532 nm laser

300400500600700800900Wavelength (nm)CF532
Technical Summary

Abs/Em Maxima: 527/558 nm
Extinction coefficient: 96,000
Molecular weight: ~ 685
Excitation laser line: 532 nm
Direct replacement for: Alexa Fluor® 532, Atto 532

Features

Designed for the 532 nm laser
Brighter than Alexa Fluor® 532
Highly water-soluble and pH-insensitive

CF®535ST Dye

An orange fluorescent dye designed for STORM super-resolution imaging

300400500600700800900Wavelength (nm)CF535ST
Technical Summary

Abs/Em Maxima: 535/568 nm
Extinction coefficient: 95,000
Molecular weight: ~728
Excitation laser line: 532 nm

Features
CF®543 Dye

An orange fluorescent dye ideal for the 543 nm laser

300400500600700800900Wavelength (nm)CF543
Technical Summary

Abs/Em Maxima: 541/560 nm
Extinction coefficient: 100,000
Molecular weight: ~ 870
Excitation laser line: 532 nm, 543 nm, or
546 nm
Direct replacement for: Alexa Fluor® 546,
TAMRA

Features

Optimized for the 543 nm laser
Yields the brightest conjugates among spectrally similar dyes
Highly water-soluble and pH-insensitive

CF®550R Dye

A unique orange/red dye for multispectral detection or FRET

300400500600700800900Wavelength (nm)CF550R
Technical Summary

Abs/Em maxima: 551/577 nm
Extinction coefficient: 100,000
Molecular weight: ~686
Excitation laser line: 532 nm or 568 nm

Features
CF®555 Dye

A bright and photostable orange-red dye

300400500600700800900Wavelength (nm)CF555
Technical Summary

Abs/Em Maxima: 555/565 nm
Extinction coefficient: 150,000
Molecular weight: ~ 901
Excitation laser line: 532 nm or 568 nm
Direct replacement for: Alexa Fluor®
555, ATTO 550, Cy®3, DyLight® 549,
Rhodamine

Features

Brighter than Cy®3
Highly water-soluble
Validated in multicolor STORM super-resolution imaging

CF®568 Dye

Outshines Alexa Fluor®568

300400500600700800900Wavelength (nm)CF568
Technical Summary

Abs/Em Maxima: 562/583 nm
Extinction coefficient: 100,000
Molecular weight: ~ 714
Excitation laser line: 532 nm or 568 nm
Direct replacement for: Alexa Fluor® 568,
ATTO 565, Rhodamine Red

Features

Yields much brighter antibody conjugates than Alexa
Fluor® 568
Extremely photostable
Excellent choice for multi-color imaging with CF®488A and CF®640R
Compatible with TIRF and multicolor STORM super resolution imaging

CF®570 Dye

Bright red fluorescent dye

300400500600700800900Wavelength (nm)