CF®405S is a blue fluorescent dye with an absorption peak wavelength matching the 405 nm blue diode laser line (Figure 1). In addition, the emission peak wavelength of the dye is well-centered within the blue detection window of BD flow cytometers. As a result, flow cytometry analysis using CF®405S dye results in much brighter signal than that using Alexa Fluor® 405 (Figure 2). CF®405S also has been validated in super-resolution imaging by SIM. See CF®405S product lines below.
CF®405S Technical Summary
- Abs/Em maxima: 404/431 nm
- Extinction coefficient: 33,000
- Molecular weight: ~1,169
- Excitation laser line: 405 nm
- Direct replacement for: Alexa Fluor® 405, Cascade Blue®, DyLight® 405
CF is a registered trademark of Biotium; Alexa Fluor, Cascade Blue, and DyLight are registered trademarks of Thermo Fisher Scientific.