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CF®594 Dye

Truly the brightest deep-red dye

Brightest deep-red conjugates
Highly photostable
Great for multi-color imaging

Figure 1. Normalized absorption and emission spectra of CF®594 goat anti-mouse IgG conjugate in PBS.

CF®594 is a deep-red fluorescent dye spectrally similar to Alexa Fluor® 594 and Texas Red® dye (Figure 1). It is significantly brighter than Alexa Fluor® 594 when conjugated to proteins, due to its high quantum yield and exceptional water solubility (Figure 2). CF®594 also is very photostable (Figure 3), making it ideal for demanding applications such as confocal microscopy. Its emission is well-separated from green dyes like CF®488A for multicolor imaging. See CF®594 product lines below.

CF®594 Technical Summary

  • Abs/Em maxima: 593/614 nm
  • Extinction coefficient: 115,000
  • Molecular weight: ~730
  • Laser line: 532 or 594 nm
  • Direct replacement for: Alexa Fluor® 594, Texas Red®, DyLight® 594

Figure 2. CF®594 yields brighter conjugates than Alexa Fluor® 594. Relative fluorescence of goat anti-mouse IgG labeled with varying degrees of labeling (DOL, or dye molecules per protein).
Figure 3. Photostability of CF®594 and AlexaFluor®594. Jurkat cells were stained with mouse anti-CD3 and CF®594 or Alexa Fluor® 594 goat anti-mouse IgG. Cells were exposed to continuous mercury arc lamp excitation through a Texas Red® filter cube. Images were captured every 15 seconds for 5 minutes. Fluorescence was normalized to time 0.

Figure 4. S. cerevisiae yeast stained with CF®594 wheat germ agglutinin (cell walls, red) and CF®488A concanavalin A (bud scars, green).
Figure 5. Neuromuscular junction in a section of rat skeletal muscle stained with CF®594 α-bungarotoxin (red). Nuclei are stained blue with DAPI.
Figure 6. Fixed oleic-acid treated HeLa cells stained with LipidSpot™ 488 (lipid droplets, green) CF®594 WGA (cell surface, red), and Hoechst 33258 (nuclei, blue).

CF is a registered trademark of Biotium, Inc. Alexa Fluor, DyLight, and Texas Red are registered trademarks of Thermo Fisher Scientific.

CF®570 & CF®583 Dyes < Previous           CF® Dyes Overview           Next > CF®594ST Dye

Mix-n-Stain CF Dye Antibody Labeling Kits

From: $95 Sizes: 5-20 ug labeling, 10-20 ug labeling, 20-50 ug labeling, 25-50 ug labeling, 50-100 ug labelingCatalog #: 92333, 92285, 92284, 92283, 92282, 92281, 92280, 92279, 92278, 92277, 92276, 92275, 92274, 92273, 92272, 92271, 92270, 92287, 92288, 92289, 92332, 92331, 92324, 92323, 92322, 92321, 92320, 92319, 92318, 92317, 92316, 92305, 92304, 92303, 92291, 92290, 92270, 92268, 92267, 92246, 92245, 92243, 92242, 92241, 92240, 92239, 92238, 92237, 92236, 92235, 92234, 92233, 92232, 92231, 92230, 92247, 92248, 92250, 92265, 92264, 92263, 92262, 92261, 92260, 92259, 92258, 92257, 92256, 92255, 92254, 92253, 92252, 92251, 92250, - 92230View allHide

Bioconjugates

Lectins, proteins, nucleotides, toxins, and other probes conjugated to our bright & photostable CF® dyes and other labels.

Primary Antibodies

Growing collection of more than 1000 monoclonal antibodies validated in IHC and other applications. Available with bright and photostable CF® dyes, R-PE, APC, PerCP, HRP, AP, biotin, or purified. Affordable 100 uL trial sizes.

CF® Dye Labeled & Biotinylated Secondary Antibodies

Many host/target species and cross-absorption options, featuring a wide spectrum of our bright & photostable CF® dyes or biotin.

CF® Dye Protein Labeling Kits

Everything you need to label and purify antibodies or other proteins. Choose from 19 bright & photostable CF® dyes or biotin.

Reactive CF® Dyes

Our bright, photostable, highly water soluble CF® dyes with a wide selection of colors and functional groups.

Additional conjugates or reactive dye forms may be added on request, contact techsupport@biotium.com to inquire.

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Faq

CF® Dyes

See our CF® Dye Quick Reference Table for a list of dyes and summary of their features. Our CF® Dye Selection Guide has more detailed information on each CF® dye, and ordering information for our various CF® dye product lines. You can download CF® dye normalized absorption and emission data in Excel format.

Category: Mix-n-Stain™ Antibody Labeling Kits, CF® Dyes

← FAQs
CF® initially was an abbreviation for Cyanine-based Fluorescent dyes. These were the first patented CF® dyes based on cyanine dye structures. Since then, our CF® dye patent portfolio has expanded to include four different fluorescent dye core structures that cover the fluorescence spectrum from UV to NIR.

Category: CF® Dyes

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The exact chemical structures of CF® dyes are currently confidential but will be fully disclosed at a later stage when pending patents become granted. In general terms, the structure of a CF® dye may be divided into two parts: a) dye core structure (i.e. the aromatic ring skeleton that defines the dye’s color or absorption/emission wavelengths), and b) core structure-modifying elements. At present, CF® dyes bear the core structures of coumarin, pyrene, rhodamine or cyanine dyes. Blue fluorescent CF® dyes are based on coumarin or pyrene dye core structure, while green to near-IR CF® dyes are based on either cyanine or rhodamine dye core structures. Core structure-modifying elements refer to various chemical attachments to the core structure and are a key aspect of the CF® dye invention that makes CF® dyes superior to other commercial dyes.

Category: CF® Dyes

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