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CF™594 Dye

CF™594 is a deep red fluorescent dye spectrally similar to Alexa Fluor® 594 and Texas Red® dye (Figure 1). When conjugated to proteins, CF™594 is significantly brighter than Alexa Fluor® 594 due to its high quantum yield and exceptional water solubility (Figure 2). CF™594 also has excellent photostability ideal for demanding applications, such as confocal and single molecular imagings (Figure 3). These properties make CF™594 the best deep-red dye for labeling proteins and nucleic acids. The dye is particularly useful to combine with our blue fluorescent CF™405 dyes, green fluorescent CF™488A and far red CF™640R for multi-color imaging.

A full selection of reactive dyes, secondary antibodies, antibody labeling kits, and other bioconjugates including phalloidins, Annexin V and α-bungarotoxin are available for CF™ dyes.

View products associated with this technology…

CF™594 conjugates, labeling kits, and reactive dyes

Technical Summary

  • Abs/Em Maxima: 593/614 nm
  • Extinction coefficient: 115,000
  • Molecular weight: ~730
  • Flow cytometry laser line: 532 or 594 nm
  • Microscopy laser line: 594 nm
  • Direct replacement for: Alexa Fluor® 594, Texas Red®, DyLight™ 594 and Cy®3.

Advantages

  • Yields the brightest antibody conjugates among spectrally similar dyes.
  • Extremely photostable
  • Highly water-soluble

Figure 1. Absorption and emission spectra of CF™594 conjugated to goat anti-mouse IgG in PBS.
Figure 1. Absorption and emission spectra of CF™594 conjugated to goat anti-mouse IgG in PBS.

Figure 2. Relative fluorescence of CF™594- and Alexa Fluor® 594-goat anti-mouse conjugates as a function of number of dye per protein or degree of labeling (DOL). Fluorescence was measured at each dye’s emission maximum in PBS using 594 nm excitation.
Figure 2. Relative fluorescence of CF™594- and Alexa Fluor® 594-goat anti-mouse conjugates as a function of number of dye per protein or degree of labeling (DOL). Fluorescence was measured at each dye’s emission maximum in PBS using 594 nm excitation.

Figure 3. Jurkat cells were stained with intracellular CD3 followed by CF594 or Alexa Fluor594 goat anti-mouse conjugates. Cells were continuously exposed using a mercury arc lamp microscope. Images were captured every 15 seconds for 5 min and fluorescence intensity of each frame was normalized to the first image.
Figure 3. Jurkat cells were stained with intracellular CD3 followed by CF594 or Alexa Fluor594 goat anti-mouse conjugates. Cells were continuously exposed using a mercury arc lamp microscope. Images were captured every 15 seconds for 5 min and fluorescence intensity of each frame was normalized to the first image.