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CF®405M Dye

Superior blue dye for the 405 nm laser

More photostable than Pacific Blue™ dye
Bright fluorescence with less spillover to the green channel
Validated in super-resolution microscopy by SIM

Figure 1. Normalized absorption and emission spectra of CF®405M goat anti-mouse IgG conjugate in PBS.

CF®405M is a blue fluorescent dye spectrally similar to Pacific Blue­™ dye. CF®405M is as bright as Pacific Blue™ dye but is significantly more photostable than the latter (Figures 2 and 3). An additional advantage of CF®405M is that it has less spillover in the 525/50 green channel than Pacific Blue™ dye due to narrower emission peak, making CF®405M a superior choice in multi-color detection applications. CF®405M also has been validated in super-resolution imaging by SIM. See CF®405M product lines below.

CF®405M Technical Summary

  • Abs/Em maxima: 408/452 nm
  • Extinction coefficient: 41,000
  • Molecular weight: ~503
  • Excitation laser line: 405 nm
  • Direct replacement for: Pacific Blue™, BD Horizon™ V450

Figure 2. CF®405M is as bright as Pacific Blue™ in flow cytometry. Jurkat cells were stained with intracellular CD3 followed by CF®405M or Pacific Blue™ goat anti-mouse IgG conjugates. Fluorescence was analyzed on a BD LSR II flow cytometer equipped with a 405 violet laser and 450/50 nm emission detection. The bars represent the relative fluorescence of the geometric means of the population of cells. Background was determined by secondary antibody staining alone.
Figure 3. CF®405M is more photostable than Pacific Blue™. CF®405M and Pacific Blue™ solutions were continuously exposed to mercury arc lamp excitation with a DAPI filter set. Images were captured every 5 seconds for one minute. Fluorescence intensity was normalized to time zero.

Figure 4. Methanol-fixed MCF-7 cells stained with mouse anti-tubulin antibody plus CF®405M goat anti-mouse secondary antibody (microtubules, cyan) and RedDot™2 far-red nuclear stain (magenta).
Figure 5. MCF-7 cells stained with CF®405M monolconal mouse anti-Cyclin B1 antibody, clone CCNB1/1098 (nuclei/nucleoli, blue) and CF®488A phalloidin (actin filaments, green).
Figure 6. HeLa cells stained with rabbit anti-COXIV + CF®488A goat anti-rabbit (mitochondria, green), mouse anti-Golgin 97 + CF®555 goat anti-mouse (Golgi, red), CF®405M phalloidin (actin, blue), and RedDot™2 (nuclei, magenta).

CF is a registered trademark of Biotium; Pacific Blue is a trademark of Thermo Fisher Scientific; BD Horizon is a trademark of BD Biosciences.

CF®405S Dye < Previous       CF® Dyes Overview        Next > CF405®L Dye

Annexin V Conjugates

From: $89 Sizes: 100 uL, 25 ug, 500 uL, 0.5 mLCatalog #: 29001, 29057-500uL, 29057-100uL, 29045-500uL, 29045-100uL, 29069, 29014, 29013, 29012, 29011, 29010, 29009, 29008, 29005, 29004, 29003, 29002, - 29070View allHide

Phalloidin Conjugates

From: $75 Sizes: 100 U, 50 U, 300 UCatalog #: 00027, 00055, 00034-T, 00040-T, 00041-T, 00042-T, 00043-T, 00044-T, 00045-T, 00046-T, 00047-T, 00048-T, 00049-T, 00050-T, 00051-T, 00052-T, 00053-T, 00054-T, 00054, 00053, 00052, 00028, 00030, 00032, 00033, 00034, 00040, 00041, 00042, 00043, 00044, 00045, 00046, 00047, 00048, 00049, 00050, 00051, - 00055-TView allHide

Streptavidin Conjugates

From: $93 Sizes: 100 uL, 200 uL, 1 mL, 1 mgCatalog #: 29030, 29056, 29065, 29066, 29072, 29044-1mL, 29044-200uL, 29048-1mL, 29048-200uL, 29071-100uL, 29071-1mL, 29049, 29043, 29041, 29031, 29032, 29033, 29034, 29035, 29036, 29037, 29038, 29039, 29040, - 29081View allHide

Wheat Germ Agglutinin (WGA) Conjugates

From: $66 Sizes: 1 mg, 5 x 1 mgCatalog #: 29021, 29024-1, 29025-1, 29026-1, 29027-1, 29028-1, 29029-1, 29059-1, 29064-1, 29077-1, 29077, 29076-1, 29023-1, 29022-1, 29021-1, 29022, 29023, 29024, 29025, 29026, 29027, 29028, 29029, 29059, 29064, 29073, - 29076View allHide

Primary Antibodies

Growing collection of more than 1000 monoclonal antibodies validated in IHC and other applications. Available with bright and photostable CF® dyes, R-PE, APC, PerCP, HRP, AP, biotin, or purified. Affordable 100 uL trial sizes.

CF® Dye Labeled & Biotinylated Secondary Antibodies

Many host/target species and cross-absorption options, featuring a wide spectrum of our bright & photostable CF® dyes or biotin.

Mix-n-Stain™ Antibody Labeling Kits

Mix-n-Stain™ kits use revolutionary technology that dramatically simplifies antibody labeling and requires no purification. The labeling is covalent and stable. Available with your choice of 24 CF® dyes, biotin, FITC, fluorescent proteins, enzymes, or haptens.

CF® Dye Protein Labeling Kits

Everything you need to label and purify antibodies or other proteins. Choose from 19 bright & photostable CF® dyes or biotin.

Reactive CF® Dyes

Our bright, photostable, highly water soluble CF® dyes with a wide selection of colors and functional groups.

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CF® Dyes

See our CF® Dye Quick Reference Table for a list of dyes and summary of their features. Our CF® Dye Selection Guide has more detailed information on each CF® dye, and ordering information for our various CF® dye product lines. You can download CF® dye normalized absorption and emission data in Excel format.

Category: Mix-n-Stain™ Antibody Labeling Kits, CF® Dyes

← FAQs
CF® initially was an abbreviation for Cyanine-based Fluorescent dyes. These were the first patented CF® dyes based on cyanine dye structures. Since then, our CF® dye patent portfolio has expanded to include four different fluorescent dye core structures that cover the fluorescence spectrum from UV to NIR.

Category: CF® Dyes

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The exact chemical structures of CF® dyes are currently confidential but will be fully disclosed at a later stage when pending patents become granted. In general terms, the structure of a CF® dye may be divided into two parts: a) dye core structure (i.e. the aromatic ring skeleton that defines the dye’s color or absorption/emission wavelengths), and b) core structure-modifying elements. At present, CF® dyes bear the core structures of coumarin, pyrene, rhodamine or cyanine dyes. Blue fluorescent CF® dyes are based on coumarin or pyrene dye core structure, while green to near-IR CF® dyes are based on either cyanine or rhodamine dye core structures. Core structure-modifying elements refer to various chemical attachments to the core structure and are a key aspect of the CF® dye invention that makes CF® dyes superior to other commercial dyes.

Category: CF® Dyes

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