CF®405M is a blue fluorescent dye spectrally similar to Pacific Blue™ dye. CF®405M is as bright as Pacific Blue™ dye but is significantly more photostable than the latter (Figures 2 and 3). An additional advantage of CF®405M is that it has less spillover in the 525/50 green channel than Pacific Blue™ dye due to narrower emission peak, making CF®405M a superior choice in multi-color detection applications. CF®405M also has been validated in super-resolution imaging by SIM. See CF®405M product lines below.
CF®405M Technical Summary
- Abs/Em maxima: 408/452 nm
- Extinction coefficient: 41,000
- Molecular weight: ~503
- Excitation laser line: 405 nm
- Direct replacement for: Pacific Blue™, BD Horizon™ V450
CF is a registered trademark of Biotium; Pacific Blue is a trademark of Thermo Fisher Scientific; BD Horizon is a trademark of BD Biosciences.