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CF™488A Dye

CF™488A is a green fluorescent dye optimally excited by the 488 nm argon laser line. Under common detection conditions, CF™488A is at least as bright as Alexa Fluor® 488. However, a major advantage of CF™488A over Alexa Fluor® 488 is that antibody conjugates prepared from the former are biologically more specific. Alexa Fluor® 488 carries multiple negative charges, which can significantly change the isoelectric point of the proteins the dye labels and consequently alter the specificity of the protein conjugates.

CF™488A, on the other hand, is minimally charged. Thus, antibody conjugates prepared from the dye ensure biological detection with high signal-to-noise ratio. Another feature of CF™488A is that the emission peak wavelength is about 10 nm shorter than that of Alexa Fluor® 488 and 15 nm shorter than that of the traditional green dye FITC (or FAM). The shorter wavelength of CF™488A offers the advantage of less fluorescence “spill-over” in the red channel in multi-color detection applications.

A full selection of reactive dyes, secondary antibodies, antibody labeling kits, and other bioconjugates including phalloidins, Annexin V and α-bungarotoxin are also available for CF™ dyes.


View products associated with this technology…

CF™488A conjugates, labeling kits, and reactive dyes


Technical Summary

  • Abs/Em Maxima: 490/515 nm
  • Extinction coefficient: 70,000
  • Molecular weight: ~910
  • Flow cytometry laser line: 488 nm
  • Microscopy laser line: 488 nm
  • Direct replacement for: Alexa Fluor® 488, DyLight™488, FITC, FAM, Cy®2

Advantages

  • Yields biologically more specific antibody conjugates and has less “spill-over” fluorescence in the red channel than Alexa Fluor® 488
  • Extremely photostable
  • Highly water-soluble and pH-insensitive
Figure 1. Absorption and emission spectra of CF™488A (blue), Alexa Fluor® 488 (green) and FITC (red) conjugated to goat anti-mouse IgG in PBS.
Figure 1. Absorption and emission spectra of CF™488A (blue), Alexa Fluor® 488 (green) and FITC (red) conjugated to goat anti-mouse IgG in PBS.

cf488-2
Figure 2. Jurkat cells were stained with 0.5 ug intracellular CD3 or isotype control (BD Biosciences) followed by 1 ug goat anti-mouse IgG conjugates from the manufacturer’s shown above. Fluorescence was analyzed on a Beckman Coulter FC-500. The bars represent the median values of the gated population of cells. The histogram depicts CF488A (green), Alexa Fluor488 (blue), DyLight488 Pierce (purple) and DyLight488 KPL (red) stained cells.
Figure 3. Cryosections (6 µm) of human control heart sections stained with anti-fibronectin followed by goat anti-rabbit IgG labeled with CF™488A, Cy™2 and Alexa Fluor® 488, respectively. Courtesy of Dr. Sawa Kostin at Max-Planck-Institute für Herz- und Lungenforschung (W.G. Kerckhoff-Institut) in Bad Nauheim (Hessen)-Germany
Figure 3. Cryosections (6 µm) of human control heart sections stained with anti-fibronectin followed by goat anti-rabbit IgG labeled with CF™488A, Cy™2 and Alexa Fluor® 488, respectively. Courtesy of Dr. Sawa Kostin at Max-Planck-Institute für Herz- und Lungenforschung (W.G. Kerckhoff-Institut) in Bad Nauheim, Hessen, Germany