CF®488A is a green fluorescent dye for the 488 nm argon laser line. CF®488A is at least as bright as Alexa Fluor® 488. However, Alexa Fluor® 488 carries multiple negative charges, which can significantly alter antibody specificity. CF®488A is minimally charged, for high specificity. CF®488A also has a shorter emission wavelength than Alexa Fluor® 488 and FITC, for less fluorescence spill-over into the red channel. CF®488A has been validated in STED and TIRF super-resolution imaging methods (learn more about CF® Dyes for Super-Resolution Imaging). See CF®488A product lines below.
CF®488A Technical Summary
- Abs/Em maxima: 490/515 nm
- Extinction coefficient: 70,000
- Molecular weight: ~914
- Excitation laser line: 488 nm
- Direct replacement for: Alexa Fluor® 488, Cy®2, DyLight® 488, fluorescein (FITC)
CF is a registered trademark of Biotium. Alexa Fluor and DyLight are registered trademarks of Thermo Fisher Scientific; Cy Dye is a registered trademark of GE Healthcare.