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CF®488A Dye

A superior green fluorescent dye

Minimally charged, for more specific conjugates
Better separation from the red channel than similar dyes
Validated in STED and TIRF Super-resolution imaging

Figure 1. Normalized absorption and emission spectra of CF®488A goat anti-mouse in PBS.

CF®488A is a green fluorescent dye for the 488 nm argon laser line. CF®488A is at least as bright as Alexa Fluor® 488. However, Alexa Fluor® 488 carries multiple negative charges, which can significantly alter antibody specificity. CF®488A is minimally charged, for high specificity. CF®488A also has a shorter emission wavelength than Alexa Fluor® 488 and FITC, for less fluorescence spill-over into the red channel. CF®488A has been validated in STED and TIRF super-resolution imaging methods (learn more about CF® Dyes for Super-Resolution Imaging). See CF®488A product lines below.

CF®488A Technical Summary

  • Abs/Em maxima: 490/515 nm
  • Extinction coefficient: 70,000
  • Molecular weight: ~914
  • Excitation laser line: 488 nm
  • Direct replacement for: Alexa Fluor® 488, Cy®2, DyLight® 488, fluorescein (FITC)

 

Figure 2. CF®488A is much more photostable than FITC. HeLa cells were stained with CF®488A phalloidin or FITC phalloidin and imaged at time zero, or after 5 seconds of exposure to excitation through a FITC filter set. FITC photobleaches almost immediately, while CF®488A is highly photostable.
Figure 3. HeLa cells stained with rabbit anti-COX IV and CF®488A goat anti-rabbit (mitochondria, green), and mouse anti-Golgin 97 and CF®555 goat anti-mouse (Golgi, red). Actin filaments are stained with CF®405 phalloidin (blue) and nuclei are stained with RedDot™2 (magenta).

CF is a registered trademark of Biotium. Alexa Fluor and DyLight are registered trademarks of Thermo Fisher Scientific; Cy Dye is a registered trademark of GE Healthcare.

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Bioconjugates

Lectins, proteins, nucleotides, toxins, and other probes conjugated to our bright & photostable CF® dyes and other labels.

Primary Antibodies

Growing collection of more than 1000 monoclonal antibodies validated in IHC and other applications. Available with bright and photostable CF® dyes, R-PE, APC, PerCP, HRP, AP, biotin, or purified. Affordable 100 uL trial sizes.

CF® Dye Labeled & Biotinylated Secondary Antibodies

Many host/target species and cross-absorption options, featuring a wide spectrum of our bright & photostable CF® dyes or biotin.

Mix-n-Stain™ Antibody Labeling Kits

Mix-n-Stain™ kits use revolutionary technology that dramatically simplifies antibody labeling and requires no purification. The labeling is covalent and stable. Available with your choice of 24 CF® dyes, biotin, FITC, fluorescent proteins, enzymes, or haptens.

CF® Dye Protein Labeling Kits

Everything you need to label and purify antibodies or other proteins. Choose from 19 bright & photostable CF® dyes or biotin.

Reactive CF® Dyes

Our bright, photostable, highly water soluble CF® dyes with a wide selection of colors and functional groups.

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Faq

CF® Dyes

See our CF® Dye Quick Reference Table for a list of dyes and summary of their features. Our CF® Dye Selection Guide has more detailed information on each CF® dye, and ordering information for our various CF® dye product lines. You can download CF® dye normalized absorption and emission data in Excel format.

Category: Mix-n-Stain™ Antibody Labeling Kits, CF® Dyes

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CF® initially was an abbreviation for Cyanine-based Fluorescent dyes. These were the first patented CF® dyes based on cyanine dye structures. Since then, our CF® dye patent portfolio has expanded to include four different fluorescent dye core structures that cover the fluorescence spectrum from UV to NIR.

Category: CF® Dyes

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The exact chemical structures of CF® dyes are currently confidential but will be fully disclosed at a later stage when pending patents become granted. In general terms, the structure of a CF® dye may be divided into two parts: a) dye core structure (i.e. the aromatic ring skeleton that defines the dye’s color or absorption/emission wavelengths), and b) core structure-modifying elements. At present, CF® dyes bear the core structures of coumarin, pyrene, rhodamine or cyanine dyes. Blue fluorescent CF® dyes are based on coumarin or pyrene dye core structure, while green to near-IR CF® dyes are based on either cyanine or rhodamine dye core structures. Core structure-modifying elements refer to various chemical attachments to the core structure and are a key aspect of the CF® dye invention that makes CF® dyes superior to other commercial dyes.

Category: CF® Dyes

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