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Fluorescent CF® Dyes CF®640R Dye

CF®640R Dye

Highly photostable far-red dye
  • Best photostability among Cy®5-like dyes
  • Yields conjugates with excellent brightness
  • Comparable with TIRF and other super-resolution techniques
CF® Dye Spectrum:
CF®350 Dye
CF®350 is a blue fluorescent dye
CF®405S Dye
Improved brightness & photostability for the 405 nm laser line
CF®405M Dye
Improved brightness & photostability for the 405 nm laser line
CF®430 & CF®440 Dyes
Photostable 405 nm-excitable dyes with green fluorescence
CF®405L Dye
A 405 nm-excitable dye with green fluorescence emission
CF®450 Dye
A spectrally unique, 405 nm-excitable green dye
CF®488A Dye
A superior green fluorescent dye
CF®503R Dye
A spectrally unique green dye ideal for spectral flow cytometry
CF®514 Dye
Alternative green fluorescent dye
CF®532 Dye
A bright green fluorescent dye for the 532 nm laser
CF®543 Dye
An orange fluorescent dye ideal for the 543 nm laser
CF®535ST Dye
A red fluorescent dye designed for STORM super-resolution imaging
CF®550R Dye
A spectrally unique orange/red dye ideal for spectral flow cytometry
CF®555 Dye
A bright and photostable orange-red dye
CF®568 Dye
Outshines Alexa Fluor®568
CF®570
Bright red fluorescent dye
CF®583 & CF®583R Dyes
Bright red fluorescent dyes
CF®594 Dye
Truly the brightest deep red dye
CF®597R Dye
Unique green-excited dye for STORM
RPE-Astral™616
Novel long Stokes shift tandem dye for flow cytometry multiplexing
CF®620R Dye
A bright and photostable far-red dye
CF®633 Dye
The best dye for 633/635 laser lines
CF®640R Dye
A highly photostable far-red dye
CF®647 Dye
A highly fluorescent far-red dye
CF®660C & CF®660R
Superior alternatives to Alexa Fluor® 660
CF®680 & CF®680R
Two outstanding 680 nm-excitable dyes
CF®700 Dye
Brighter alternative to Alexa Fluor® 700
CF®710 Dye
Near-infrared CF® Dye with exceptional photostability
CF®725 Dye
Near-infrared CF® Dye with exceptional photostability
CF®740 Dye
Near-infrared CF® Dye with exceptional photostability
CF®750 Dye
Near-infrared CF® Dye
CF®770 Dye
Near-infrared CF® Dye
CF®790 Dye
Near-infrared CF® Dye
APC-Astral™813
Novel long Stokes shift tandem dye for flow cytometry multiplexing
CF®800 Dye
Near-infrared CF® Dye
CF®820 Dye
Near-infrared CF® Dye
CF®850 Dye
Near-infrared CF® Dye
CF®870 Dye
Near-infrared CF® Dye

CF®640R is a rhodamine-based far-red dye with excitation/emission similar to Cy®5 and Alexa Fluor® 647 but offers much greater brightness and exceptional photostability (Figures 1–4). Unlike the cyanine-based Cy®5 and Alexa Fluor® 647, which are prone to photobleaching, CF®640R maintains signal intensity in demanding applications. It also outperforms ATTO 647N, producing brighter antibody conjugates and enabling high-degree labeling without quenching due to its water solubility. These qualities make CF®640R ideal for confocal microscopy, single-molecule imaging, and super-resolution techniques like TIRF and FLIMP (learn more about CF® Dyes for Super-Resolution Microscopy).

See products available with CF®640R. Additional reactive dyes or conjugates may be added on request, contact techsupport@biotium.com to inquire.

CF®640R Features

  • The best photostability among Cy®5-like dyes
  • Yields highly fluorescent protein conjugates
  • Water-soluble and pH-insensitive
  • Compatible with TIRF and FLIMP super-resolution imaging

CF®640R Technical Summary

  • Abs/Em maxima: 642/662 nm
  • Extinction coefficient: 105,000
  • Molecular weight: ~832
  • Excitation laser line: 633, 635 or 640 nm
  • Alternative for: Alexa Fluor® 647, ATTO 647N, Cy®5, DyLight® 649
  • Fluorescence lifetime:
    τ (ns)/free acid in PBS pH 7.4, ε (ns) = 2.38 ± 0.05
    τ (ns) /S.Ab§ = 1.557
    Lifetime data obtained via customer communication under different experimental conditions and imaging setup.

Visit our FAQ for more fluorescence lifetime information on CF® Dyes.

Figure 1. Normalized absorbance and emission spectra of CF®640R goat anti-mouse conjugate in PBS.
Figure 2. CF®640R yields brighter antibody conjugates than other far-red dyes. Jurkat cells were stained with mouse anti-CD3 antibody or no primary antibody and... See More
Figure 3. CF®640R is much more photostable than Alexa Fluor® 647. Jurkat cells were stained with mouse anti-CD3 antibody followed by goat anti-mouse secondary a... See More
Figure 4. Relative photostability of CF®640R and Alexa Fluor® 647 fluorescence. HeLa cells were stained with mouse anti-tublin antibody and goat anti-mouse conj... See More
Figure 5. Section of rat intestine stained with CF®640R phalloidin (actin filaments, magenta) and NucSpot® 470 (nuclei, green).
HeLa stained with CellBrite® Fix 555 (red), fixed and stained with CF®640R anti-Mitochondrial Marker clone 113-1 (cyan).
Figure 7. MCF-7 cells stained with CF®640R anti-Cyclin B1 (nuclei/nucleoli, magenta), CF®488A phalloidin (actin filaments, green), and Hoechst (DNA, blue).

Helpful Tools & Resources

Visualize and export the fluorescent dye spectrum of any CF® Dye or other dyes from Biotium.
Learn the history of fluorophores in life science research as well as the development and advantages of CF® Dyes.
Check out our handy fluorophore selection tool to help choose the best CF® Dye for your next experiment.
Download our single page reference guide for all CF® Dye fluorophores. Includes recommended channels, applications, relative brightness, and photostability.
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CF®633 Dye
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CF®647 Dye

CF is a registered trademark of Biotium Inc. Alexa Fluor and DyLight are registered trademarks of Thermo Fisher Scientific; Cy Dye is a registered trademark of Cytiva.

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