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CF™543 Dye

CF™543 is an orange colored fluorescent dye with absorption and emission maxima at 541 and 560 nm, respectively (Figure 1). The dye is based on a new class of rhodamine dyes invented by Biotium scientists. Unlike traditional rhodamine dyes, CF™543 is not only bright and photostable but also highly water-soluble. As a result, CF™543 antibody conjugates retain excellent solubility and yield the brightest fluorescence among antibody conjugates prepared from spectrally similar dyes. For example, CF™543 labeled goat anti-mouse IgG is significantly brighter than the antibody labeled with Alexa Fluor® 546 at similar degree of labeling (Figure 3).
A full selection of reactive dyes, secondary antibodies, antibody labeling kits, and other bioconjugates including phalloidins, Annexin V and α-bungarotoxin are available for CF™ dyes.

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CF™543 conjugates, labeling kits, and reactive dyes

 

  • Abs/Em Maxima: 541/560 nm
  • Extinction coefficient: 100,000
  • Molecular weight: ~870
  • Flow cytometry laser line: 532 nm
  • Microscopy laser line: 543, 546 nm
  • Direct replacement for: Alexa Fluor® 546, Tetramethylrhodamine (TAMRA).

Advantages

  • Significantly brighter than Alexa Fluor® 546
  • Highly water-soluble and pH-insensitive
Figure 1. Absorption and emission spectra of CF™543 conjugated to goat anti-mouse IgG in PBS.
Figure 1. Absorption and emission spectra of CF™543 conjugated to goat anti-mouse IgG in PBS.
CF543 GAM, CF488A M&S-icc
Figure 2. HeLa cells stained with mouse anti-tubulin followed by CF543 goat anti-mouse IgG (microtubules, red), followed by CF488A Mix-n-Stain labeled mouse anti-transferrin receptor antibody (endosomes/plasma membrane, green).
Figure 3. Comparison of relative fluorescence of goat anti-mouse IgG antibody labeled with CF543 and Alexa Fluor 546, respectively, at various degree of labeling (DOL) (number of dye/protein) in PBS
Figure 3. Comparison of relative fluorescence of goat anti-mouse IgG antibody labeled with CF543 and Alexa Fluor 546, respectively, at various degree of labeling (DOL) (number of dye/protein) in PBS