CF®543 is an orange fluorescent dye with absorption and emission maxima at 541 and 560 nm, respectively (Figure 1). The dye is based on a new class of rhodamine dyes invented by Biotium scientists. Unlike traditional rhodamine dyes, CF®543 is not only bright and photostable but also highly water-soluble. As a result, CF®543 antibody conjugates retain excellent solubility and yield the brightest fluorescence among spectrally similar dyes like Alexa Fluor® 546 (Figure 2). See CF®543 product lines below.
CF®543 Technical Summary
- Abs/Em maxima: 541/560 nm
- Extinction coefficient: 100,000
- Molecular weight: ~870
- Laser line: 532 nm, 543, or 546 nm
- Direct replacement for: Alexa Fluor® 546, Tetramethylrhodamine (TAMRA)
CF is a registered trademark of Biotium, Inc; Alexa Fluor is a registered trademark of Thermo Fisher Scientific.