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CF™568 Dye

CF™568 is a red fluorescent dye with an excitation spectrum optimally matching the 568 nm line of the Ar-Kr mixed-gas laser (Figure 1). Antibody conjugates of the dye are much brighter than those of Alexa Fluor® 568. In addition, the photostability of CF™568 is superior to that of Alexa Fluor® 568, making CF™568 a much better choice for demanding applications, such as confocal and single molecule imaging.
A full selection of reactive dyes, secondary antibodies, antibody labeling kits, and other bioconjugates including phalloidins, Annexin V and α-bungarotoxin are available for CF™ dyes.

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CF™568 conjugates, labeling kits, and reactive dyes

 

Technical Summary

  • Abs/Em Maxima: 562/583 nm
  • Extinction coefficient: 100,000
  • Molecular weight: ~714
  • Flow cytometry laser line: 532 nm
  • Microscopy laser line: 568 nm
  • Direct replacement for: Alexa Fluor® 568, Lissamine rhoda- mine B, ROX

Advantages

  • Yields much brighter antibody conjugates than Alexa Fluor® 568
  • Extremely photostable
  • Highly water-soluble
Figure 1. Absorption and emission spectra of CF™568 conjugated to goat anti-mouse IgG in PBS.
Figure 1. Absorption and emission spectra of CF™568 conjugated to goat anti-mouse IgG in PBS.
Figure 2. Jurkat cells were stained with intracellular CD3 or isotype control followed by goat anti-mouse IgG conjugates. Fluorescence was analyzed on a BD FACS Calibur in the FL2 channel. The bars represent the relative fluorescence of the geometric means of the population of cells.
Figure 2. Jurkat cells were stained with intracellular CD3 or isotype control followed by goat anti-mouse IgG conjugates. Fluorescence was analyzed on a BD FACS Calibur in the FL2 channel. The bars represent the relative fluorescence of the geometric means of the population of cells.
Figure 3. Jurkat cells were stained with intracellular biotin-CD3 followed by streptavidin CF568 or Alexa Fluor568 conjugates. Cells were continuously exposed using a mercury arc lamp microscope. Images were captured every 15 seconds for 5 min and fluorescence intensity of each frame was normalized to the first image.
Figure 3. Jurkat cells were stained with intracellular biotin-CD3 followed by streptavidin CF568 or Alexa Fluor568 conjugates. Cells were continuously exposed using a mercury arc lamp microscope. Images were captured every 15 seconds for 5 min and fluorescence intensity of each frame was normalized to the first image.