Excellent photostability for demanding imaging applications
Compatible SIM, TIRF, and multicolor STORM
CF®568 is a red fluorescent dye with an excitation spectrum optimally matching the 568 nm laser line. Antibody conjugates of the dye are much brighter than those of Alexa Fluor® 568. CF®568 is also more photostable than Alexa Fluor® 568, making CF®568 a much better choice for demanding applications, such as confocal and single molecule imaging. CF®568 has been validated in super-resolution imaging by SIM, TIRF, and multicolor STORM, and produces better STORM images than Cy®3b (learn more about CF® Dyes for Super-Resolution Imaging). See CF®568 product lines below.
CF®568 Technical Summary
Abs/Em maxima: 562/583 nm
Extinction coefficient: 100,000
Molecular weight: ~714
Excitation laser line: 532 nm or 568 nm
Direct replacement for: Alexa Fluor® 568, ATTO 565, Rhodamine Red
CF is a registered trademark of Biotium, Inc; Alexa Fluor is a registered trademark of Thermo Fisher Scientific.
Growing collection of more than 1000 monoclonal antibodies validated in IHC and other applications. Available with bright and photostable CF® dyes, R-PE, APC, PerCP, HRP, AP, biotin, or purified. Affordable 100 uL trial sizes.
Wide selection of species reactivities and anti-tag antibodies. Choose from more than 20 CF® dyes, R-PE, APC, HRP, AP or biotin. Highly cross-adsorbed, F(ab’)2 fragments, and isotype-specific options. Affordable 50 uL trial sizes.
Mix-n-Stain™ kits use revolutionary technology that dramatically simplifies antibody labeling and requires no purification. The labeling is covalent and stable. Available with your choice of 24 CF® dyes, biotin, FITC, fluorescent proteins, enzymes, or haptens.
CF® initially was an abbreviation for Cyanine-based Fluorescent dyes. These were the first patented CF® dyes based on cyanine dye structures. Since then, our CF® dye patent portfolio has expanded to include four different fluorescent dye core structures that cover the fluorescence spectrum from UV to NIR.
The exact chemical structures of CF® dyes are currently confidential but will be fully disclosed at a later stage when pending patents become granted. In general terms, the structure of a CF® dye may be divided into two parts: a) dye core structure (i.e. the aromatic ring skeleton that defines the dye’s color or absorption/emission wavelengths), and b) core structure-modifying elements. At present, CF® dyes bear the core structures of coumarin, pyrene, rhodamine or cyanine dyes. Blue fluorescent CF® dyes are based on coumarin or pyrene dye core structure, while green to near-IR CF® dyes are based on either cyanine or rhodamine dye core structures. Core structure-modifying elements refer to various chemical attachments to the core structure and are a key aspect of the CF® dye invention that makes CF® dyes superior to other commercial dyes.