CF®583 & CF®583R Dyes
Bright red fluorescent dyes
CF®350 is a blue fluorescent dye
Abs/Em Maxima: 347/448 nm
Extinction coefficient: 18,000
Molecular weight: ~ 496
Excitation source: UV
Replaces: Alexa Fluor® 350, AMCA,
DyLight® 350
Brighter and more photostable than AMCA
Direct replacement for Alexa Fluor® 350
Highly water soluble and pH-insensitive
Improved brightness & photostability for the 405 nm laser line
Abs/Em Maxima: 404/431 nm
Extinction coefficient: 33,000
Molecular weight: ~ 1,169
Excitation laser line: 405 nm
Replaces: Alexa Fluor® 405, Cascade Blue®,
DyLight® 405
Brighter than Alexa Flour® 408
Improved brightness & photostability for the 405 nm laser line
Abs/Em Maxima: 408/452 nm
Extinction coefficient: 41,000
Molecular weight: ~ 503
Excitation laser line: 405 nm
Replaces: Pacific Blue®, BD Horizon™ V450
More photostable than Pacific Blue®, with less spill-over in the green channel
An excellent choice for super-resolution imaging by SIM
A 405 nm-excitable dye with orange fluorescence emission
Abs/Em Maxima: 395/545 nm
Extinction coefficient: 24,000
Molecular weight: ~1573
Excitation laser line: 405 nm
Replaces: Pacific Orange®
Expands potential detection channels for the 405 nm laser line
Photostable 405 nm-excitable dyes with green fluorescence
CF®430
Abs/Em Maxima: 426/498 nm
Extinction coefficient: 40,000
Molecular weight: ~429
Excitation laser line: 405 nm
Replaces: Pacific Green®, BD Horizon™ V500,
Krome Orange™
CF®440
Abs/Em Maxima: 440/515 nm
Extinction coefficient: 40,000
Molecular weight: ~716
Excitation laser line: 405 nm
Replaces: Alexa Fluor® 430
Photostable dyes suitable for microscopy
Perfect match for the CFP filter set
Suitable for flow cytometry in the AmCyan channel
Highly water soluble and pH-insensitive
A green fluorescent dye with unique spectral properties
Abs/Em Maxima: 450/538 nm
Extinction coefficient: 40,000
Molecular weight: ~689
Excitation laser line: 405 nm
A superior green fluorescent dye
Abs/Em Maxima: 490/515 nm
Extinction coefficient: 70,000
Molecular weight: ~914
Excitation laser line: 488 nm
Replaces: Alexa Fluor® 488, DyLight® 488,
fluorescein (aka FITC, FAM), Cy®2
Minimally charged, for less non-specific binding than Alexa Fluor® 488
Narrower emission spectrum for less bleed to red
Very photostable
Compatible with super-resolution imaging by TIRF
Highly water soluble and pH-insensitive
A unique green dye or multispectral detection or FRET
Abs/Em maxima: 503/532 nm
Extinction coefficient: 90,000
Molecular weight: ~1100
Excitation laser line: 488 nm
Direct replacement for: ATTO 488
Alternative green fluorescent dye
Abs/Em Maxima: 516/548 nm
Extinction coefficient: 105,000
Molecular weight: ~1216
Excitation laser line: 488 nm
Replaces: Alexa Fluor® 514
Image using the same settings as FITC or CF®488A
Can be distinguished from CF®488A in the same specimen by spectral imaging and linear unmixing
A bright orange fluorescent dye for the 532 nm laser
Abs/Em Maxima: 527/558 nm
Extinction coefficient: 96,000
Molecular weight: ~ 685
Excitation laser line: 532 nm
Direct replacement for: Alexa Fluor® 532, Atto 532
Designed for the 532 nm laser
Brighter than Alexa Fluor® 532
Highly water-soluble and pH-insensitive
An orange fluorescent dye designed for STORM super-resolution imaging
Abs/Em Maxima: 535/568 nm
Extinction coefficient: 95,000
Molecular weight: ~728
Excitation laser line: 532 nm
An orange fluorescent dye ideal for the 543 nm laser
Abs/Em Maxima: 541/560 nm
Extinction coefficient: 100,000
Molecular weight: ~ 870
Excitation laser line: 532 nm, 543 nm, or
546 nm
Direct replacement for: Alexa Fluor® 546,
TAMRA
Optimized for the 543 nm laser
Yields the brightest conjugates among spectrally similar dyes
Highly water-soluble and pH-insensitive
A unique orange/red dye for multispectral detection or FRET
Abs/Em maxima: 551/577 nm
Extinction coefficient: 100,000
Molecular weight: ~686
Excitation laser line: 532 nm or 568 nm
A bright and photostable orange-red dye
Abs/Em Maxima: 555/565 nm
Extinction coefficient: 150,000
Molecular weight: ~ 901
Excitation laser line: 532 nm or 568 nm
Direct replacement for: Alexa Fluor®
555, ATTO 550, Cy®3, DyLight® 549,
Rhodamine
Brighter than Cy®3
Highly water-soluble
Validated in multicolor STORM super-resolution imaging
Outshines Alexa Fluor®568
Abs/Em Maxima: 562/583 nm
Extinction coefficient: 100,000
Molecular weight: ~ 714
Excitation laser line: 532 nm or 568 nm
Direct replacement for: Alexa Fluor® 568,
ATTO 565, Rhodamine Red
Yields much brighter antibody conjugates than Alexa
Fluor® 568
Extremely photostable
Excellent choice for multi-color imaging with CF®488A and CF®640R
Compatible with TIRF and multicolor STORM super resolution imaging
Bright red fluorescent dye
CF®570 Technical Summary
Abs/Em maxima: 568/591 nm
Extinction coefficient: 150,000
Molecular weight: ~2998
Excitation laser line: 561 to 568 nm
Replaces: Alexa Fluor® 568, ATTO 565, DY-560, Rhodamine Red
Bright red fluorescent dyes
CF®583 Technical Summary
Abs/Em maxima: 583/606 nm
Extinction coefficient: 150,000
Molecular weight: ~3127
Excitation laser line: 561 to 568 nm
Replaces: Cy®3.5
CF®583R Technical Summary
Abs/Em maxima: 586/609 nm
Extinction coefficient: 100,000
Molecular weight: ~773
Excitation laser line: 561 to 568 nm
Replaces: Cy®3.5, Texas Red®
Spectrally similar red fluorescent dyes with exceptional brightness
Serves as a replacement for Cy®3.5 and Texas Red®
Rhodamine-based CF®583R can be used as an excellent energy acceptor for FRET when paired with R-PE
Truly the brightest deep red dye
Abs/Em Maxima: 593/614 nm
Extinction coefficient: 115,000
Molecular weight: ~730
Excitation laser line: 532 nm, 568 nm or
594 nm
Replaces: Alexa Fluor® 594, DyLight® 594,
Texas Red®
Yields the brightest antibody conjugates among spectrally
similar dyes.
Excellent choice for multicolor imaging with green dyes like CF®488A
Extremely photostable
Also see CF®594ST, a version of CF®594 engineered specifically for STORM microcopy
Deep red dye for STORM
Abs/Em maxima: 593/614 nm
Extinction coefficient: 115,000
Molecular weight: ~868
Excitation laser line: 532 to 568 nm
A bright and photostable far-red dye