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Can GelRed® and GelGreen® be used to stain ssDNA or RNA?

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GelRed® and GelGreen® can be used to stain both ssDNA and RNA, but GelRed® is about 5 times more sensitive for single-stranded nucleic acids than GelGreen®. Titration assays using a fluorescence microplate reader showed that the fluorescence signal of GelRed® bound to ssDNA and RNA is about half that of GelRed® bound to dsDNA.


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