How long does it take to finish the whole process?

For the Mix-n-Stain™ CF® Dye, biotin, Cyanine Dye, FITC, and hapten antibody labeling kits it takes 30 seconds or less to mix the components (antibody, dye and reaction buffer). After another 15 minutes of hands-free reaction time, you are done! You can use the antibody right away to stain your sample for microscopy, flow cytometry, Western analysis, or other applications requiring fluorescently labeled antibodies.