FAQs

FAQs

Why am I seeing smeared or smiling DNA band(s) or discrepant DNA migration?

Many customers use GelRed® or GelGreen® precast gels for convenience. However, because GelRed® and GelGreen® are high affinity...

FAQs

Why do I see weak fluorescence, decreased dye performance over time, or a film of dye remaining on the gel after post-staining?

There are a few possibilities: The dye may have precipitated out of solution. Heat the GelRed® or GelGreen®...

FAQs

Does post-staining require a de-staining step?

No, but de-staining with water can be performed if background is high.

FAQs

Can GelRed® and GelGreen® be used to stain ssDNA or RNA?

GelRed® and GelGreen® can be used to stain both ssDNA and RNA, but GelRed® is about 5 times...

FAQs

What instruments can be used to detect GelRed® and GelGreen®?

GelRed® is compatible with a standard UV transilluminator (302 or 312 nm). GelGreen® has sufficient absorption between 250-300...

FAQs

What emission filters are suitable for use with GelRed® and GelGreen®?

Use the ethidium bromide filter for GelRed®; use a SYBR® Green or yellow filter for GelGreen®. Alternatively, a...