CellBrite® Cytoplasmic Membrane Dyes

CellBrite® Cytoplasmic Membrane Labeling Kits can be used to label cell cytoplasmic membranes with fluorescent dyes, available in blue, green, orange, and red. The labeling is nontoxic and suitable for long-term tracking of cells. The dyes also can be used to stain membranes of formaldehyde-fixed cells.

CellBrite® stains are based on lipophilic carbocyanine dyes: CellBrite® Blue is based on DiB, CellBrite® Green is based on Neuro-DiO, CellBrite® Orange is based on DiI, and CellBrite® Red is based on DiD. Carbocyanine dyes label cytoplasmic membrane and intracellular membrane structures efficiently and permanently. They have been used as tracers in cell fusion, cell adhesion, and cell migration applications due to their properties of low cytotoxicity and high resistance to intercellular transfer. By combining multiple CellBrite® Cytoplasmic Membrane Stains, one can label multiple cell populations with different colors for studies of cell-cell interactions.

Note: CellBrite® Dyes primarily localize to cell surface membranes shortly after staining. However, in live cells the stained membranes become internalized by endocytosis, so staining becomes primarily intracellular over the course of a few hours in culture. See “Find the Right Stain for Your Application” below, and our Membrane Staining & Imaging Tech Tip to learn more.

Biotium’s CellBrite® Cytoplasmic Membrane Dyes are dye delivery solutions that can be added directly to normal culture media to uniformly label suspended or adherent cells in culture. CellBrite® Dyes allow cell populations to be marked in distinctive fluorescent colors for identification after mixing. Double labeling can identify cells that have fused or formed stable clusters.

We also offer CellBrite® NIR Cytoplasmic Membrane Dyes with emission in the far-red/near-infrared region for imaging by either microscopy or near-infrared imaging devices.

Find the Right Stain for Your Application

CellBrite® Dyes are non-toxic and stain cells very stably. They can be used to track cells for days to weeks. However, over time the dyes will be internalized by endocytosis, resulting in labeling of intracellular vesicles. A few hours after staining, the dyes will no longer outline the plasma membrane, but will be localized inside the cell. For long-term imaging of cell surfaces in culture we recommend our CellBrite® Steady Membrane Staining Kits which distribute between the cell surface and intracellular compartments, so cells retain cell surface staining over time. For long-term visualization of cell morphology in culture, our stable, non-toxic ViaFluor® SE Cell Proliferation Kits may also be a suitable alternative. ViaFluor® SE dyes covalently label cells throughout the cytoplasm and can be tracked for several days or longer by microscopy or flow cytometry. See our Tech Tip: Using ViaFluor® SE Stains for Cell Tracing and Co-Culture to learn more.

CellBrite® Dyes can be used to stain formaldehyde-fixed cells, but the staining has poor tolerance for methanol fixation or detergent permeabilization. However, permeabilizing fixed cells before staining with CellBrite® Dyes can give good results, see our Tech Tip: Combining Lipophilic Membrane Dyes with Immunofluorescence. For staining formaldehyde-fixed cells we strongly recommend using CytoLiner™ Fixed Cell Membrane Stains which offer more robust and consistent results over the CellBrite® Cytoplasmic Membrane Dyes.

For membrane stains that withstand fixation with either formaldehyde or methanol, as well as detergent permeabilization after staining, see our CellBrite® Fix Membrane Stains and MemBrite® Fix Cell Surface Staining Kits.

CellBrite® Dyes do not stain yeast or bacteria. See our Cellular Stains Table for more information on how our dyes stain various organisms.

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