CellBrite™ Cytoplasmic Membrane Labeling Kits are designed to label cell cytoplasmic membranes with fluorescent dyes, available in blue, green, orange, and red. The labeling is stable and nontoxic, suitable for long-term tracking of cells. By combining multiple CellBrite™ Cytoplasmic Membrane Stains, one can label multiple cell populations with different colors for studies of cell-cell interactions.
CellBrite™ stains are based on lipophilic carbocyanine dyes: CellBrite™ Blue is based on DiB, CellBrite™ Green is based on Neuro-DiO, CellBrite™ Orange is based on DiI, and CellBrite™ Red is based on DiD. Carbocyanine dyes label cytoplasmic membrane and intracellular membrane structures efficiently and permanently. They have been used as tracers in cell–cell fusion, cellular adhesion, and migration applications due to their properties of low cytotoxicity and high resistance to intercellular transfer.
Biotium’s CellBrite™ Cytoplasmic Membrane Dyes are dye delivery solutions that can be added directly to normal culture media to uniformly label suspended or adherent cells in culture. CellBrite™ dyes allow cell populations to be marked in distinctive fluorescent colors for identification after mixing. Double labeling can identify cells that have fused or formed stable clusters.
We also offer CellBrite™ NIR Cytoplasmic Membrane Dyes with emission in the far-red/near-infrared region for imaging by either microscopy or near-infrared imaging devices.
- CellBrite™ fluorescent membrane dye pre-dissolved in an optimized staining solution*
- Loading buffer (CellBrite™ Blue only)
Find the Right Stain for Your Application
CellBrite™ dyes can be used to stain live cells or formaldehyde-fixed cells. Live cells can be fixed in formaldehyde after staining as well, but the staining has poor tolerance for methanol fixation or detergent permeabilization. However, permeabilizing fixed cells before staining with CellBrite™ dyes can give good results, see our Tech Tip: Combining Lipophilic Membrane Dyes with Immunofluorescence. For membrane stains that withstand fixation with either formaldehyde or methanol, as well as detergent permeabilization after staining, see our CellBrite™ Fix Membrane Stains and MemBrite™ Fix Cell Surface Staining Kits.
CellBrite™ dyes do not stain yeast or bacteria. See our Cellular Stains Table for more information on how our dyes stain various organisms.
CellBrite™ dyes are non-toxic and stain cells very stably. They can be used to track cells for days to weeks. However, over time the dyes will be internalized by endocytosis, resulting in labeling of intracellular vesicles. By 24 hours after staining, the dyes will no longer outline the plamsa membrane, but will be localized inside the cell. For long-term visualization of cell morphology in culture, our stable, non-toxic ViaFluor® SE Cell Proliferation Kits may be a more suitable alternative. ViaFluor® SE dyes covalently label cells throughout the cytoplasm and can be tracked for several days or longer by microscopy or flow cytometry. See our Tech Tip: Using ViaFluor® SE Stains for Cell Tracing and Co-Culture to learn more.
|Catalog No.||Membrane Dye||Abs/Em
|30024||CellBrite Blue ||366/441 nm
|30021||CellBrite Green ||484/501 nm
|30022||CellBrite Orange||549/565 nm
|30023||CellBrite Red||644/665 nm
* CellBrite™ Green, CellBrite™ Orange, and CellBrite™ Red are supplied in ethanol. CellBrite Blue dye (DiB Labeling Solution) was previously supplied as a solution in ethanol. The dye is now formulated in DMSO for better solubility.
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Cat#30024 CellBrite Blue
1. European Journal of Medicinal Chemistry (2017), https://doi.org/10.1016/j.ejmech.2017.11.004
2. Journal of the American Chemical Society (2018), doi:10.1021/jacs.7b12408