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CellBrite Cytoplasmic Membrane Dyes

CellBrite™ Cytoplasmic Membrane Labeling Kits are designed to label cell cytoplasmic membranes with fluorescent dyes, available in blue, green, orange, and red. The labeling is stable and nontoxic, suitable for long-term tracking of cells.

CellBrite Orange
CellBrite Spectra
Catalog #

30021, 30022, 30023, - 30024View allHide

Size

1 mL, 50 assays

Dye color

CellBrite Blue, CellBrite Red, CellBrite Orange, CellBrite Green

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1 mL
50 assays
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Product Description

CellBrite™ Cytoplasmic Membrane Labeling Kits are designed to label cell cytoplasmic membranes with fluorescent dyes, available in blue, green, orange, and red. The labeling is stable and nontoxic, suitable for long-term tracking of cells. By combining multiple CellBrite™ Cytoplasmic Membrane Stains, one can label multiple cell populations with different colors for studies of cell-cell interactions.

CellBrite™ stains are based on lipophilic carbocyanine dyes: CellBrite™ Blue is based on DiB, CellBrite™ Green is based on Neuro-DiO, CellBrite™ Orange is based on DiI, and CellBrite™ Red is based on DiD. Carbocyanine dyes label cytoplasmic membrane and intracellular membrane structures efficiently and permanently. They have been used as tracers in cell–cell fusion, cellular adhesion, and migration applications due to their properties of low cytotoxicity and high resistance to intercellular transfer.

Biotium’s CellBrite™ Cytoplasmic Membrane Dyes are dye delivery solutions that can be added directly to normal culture media to uniformly label suspended or adherent cells in culture. CellBrite™ dyes allow cell populations to be marked in distinctive fluorescent colors for identification after mixing. Double labeling can identify cells that have fused or formed stable clusters.

We also offer CellBrite™ NIR Cytoplasmic Membrane Dyes with emission in the far-red/near-infrared region for imaging by either microscopy or near-infrared imaging devices.

Kits include:

  • CellBrite™ fluorescent membrane dye pre-dissolved in an optimized staining solution
  • Loading buffer (CellBrite™ Blue only)

For more information, download the product information sheet, or see Frequently Asked Questions about CellBrite™ Cytoplasmic Membrane Stains.

Product Information

Catalog No.Membrane DyeAbs/Em
30024CellBrite™ Blue 366/441 nm
30021CellBrite™ Green 484/501 nm
30022CellBrite™ Orange549/565 nm
30023CellBrite™ Red644/665 nm

References

1. J Cell Biol 103, 171 (1986).
2. J Cell Biol 135,63 (1996).
3.Annals of Anatomy (2014), http://dx.doi.org/10.1016/j.aanat.2014.05.038
4. Veterinary and Comparative Oncology (2016), doi: 10.1111/vco.12236

 

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Support & Faq

CellBrite™ Cytoplasmic Membrane Stains

CellBrite cytoplasmic membrane stains are lipophilic carbocyanine dyes. These dyes undergo an increase in fluorescence when they insert into lipid bilayers. Lipophilic carbocyanine dyes stably label the plasma membrane and other intracellular membranes of cells. They also can be used to stain artificial lipid bilayers.

Category: CellBrite™ Cytoplasmic Membrane Stains

← FAQs

Lipophilic carbocyanine dyes have been used to stain neuronal cells in culture for several weeks, and in vivo for up to a year. The dyes do not appreciably affect cell viability, and do not readily transfer between cells with intact membranes, allowing cell migration and tracking studies in mixed populations. Stability of labeling may vary between cell types, depending on rates of membrane turnover or cell division.

Category: CellBrite™ Cytoplasmic Membrane Stains

← FAQs

Cells can be fixed with formaldehyde after labeling with CellBrite dyes. Lipophilic carbocyanine dyes like the CellBrite dyes have also been used to stain cells or tissues after formaldehyde fixation. Permeabilization of cells with detergents or solvents, or mounting medium containing glycerol may adversely affect staining. Permeabilization with digitonin (10 ug/mL to 1 mg/mL) has been reported to be compatible with lipophilic carbocyanine dye staining.

Category: CellBrite™ Cytoplasmic Membrane Stains

← FAQs

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Shipping/Shelf-life

Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions. Expedited shipment may be requested at time of checkout. Please note that products with recommended storage at 4°C or -20°C may ship at ambient temperature. This will not affect product performance. When you receive the product, place it under the recommended storage conditions.