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CellBrite Cytoplasmic Membrane Dyes

CellBrite™ Cytoplasmic Membrane Labeling Kits are designed to label cell cytoplasmic membranes with fluorescent dyes, available in blue, green, orange, and red. The labeling is stable and nontoxic, suitable for long-term tracking of cells.

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CellBrite SpectraCellBrite Blue staining of HeLa cell membranes. A. Live cells. B. Cells stained live, then fixed with formaldehyde. C. Cells fixed with formaldehyde, then stained.Live HeLa cells stained with CellBrite Green, showing cell surface and endosomal staining.Live HeLa cells stained with CellBrite Orange.Live HeLa cells stained with CellBrite Red.
Catalog #

30021, 30022, 30023, - 30024View allHide

Size

1 mL, 50 assays

Dye color

CellBrite Blue, CellBrite Red, CellBrite Orange, CellBrite Green

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50 assays
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Product Description

CellBrite™ Cytoplasmic Membrane Labeling Kits are designed to label cell cytoplasmic membranes with fluorescent dyes, available in blue, green, orange, and red. The labeling is stable and nontoxic, suitable for long-term tracking of cells. By combining multiple CellBrite™ Cytoplasmic Membrane Stains, one can label multiple cell populations with different colors for studies of cell-cell interactions.

CellBrite™ stains are based on lipophilic carbocyanine dyes: CellBrite™ Blue is based on DiB, CellBrite™ Green is based on Neuro-DiO, CellBrite™ Orange is based on DiI, and CellBrite™ Red is based on DiD. Carbocyanine dyes label cytoplasmic membrane and intracellular membrane structures efficiently and permanently. They have been used as tracers in cell–cell fusion, cellular adhesion, and migration applications due to their properties of low cytotoxicity and high resistance to intercellular transfer.

Biotium’s CellBrite™ Cytoplasmic Membrane Dyes are dye delivery solutions that can be added directly to normal culture media to uniformly label suspended or adherent cells in culture. CellBrite™ dyes allow cell populations to be marked in distinctive fluorescent colors for identification after mixing. Double labeling can identify cells that have fused or formed stable clusters.

We also offer CellBrite™ NIR Cytoplasmic Membrane Dyes with emission in the far-red/near-infrared region for imaging by either microscopy or near-infrared imaging devices.

Kits include:

  • CellBrite™ fluorescent membrane dye pre-dissolved in an optimized staining solution
  • Loading buffer (CellBrite™ Blue only)

For more information, download the product information sheet, or see Frequently Asked Questions about CellBrite™ Cytoplasmic Membrane Stains.

Product Information

Catalog No.Membrane DyeAbs/Em
30024CellBrite™ Blue 366/441 nm
30021CellBrite™ Green 484/501 nm
30022CellBrite™ Orange549/565 nm
30023CellBrite™ Red644/665 nm

References

1. J Cell Biol 103, 171 (1986).
2. J Cell Biol 135,63 (1996).
3.Annals of Anatomy (2014), http://dx.doi.org/10.1016/j.aanat.2014.05.038
4. Veterinary and Comparative Oncology (2016), doi: 10.1111/vco.12236

Cat#30024 CellBrite Blue
1. European Journal of Medicinal Chemistry (2017), https://doi.org/10.1016/j.ejmech.2017.11.004

 

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Support & Faq

CellBrite™ Cytoplasmic Membrane Stains

CellBrite™ and CellBrite™ Fix dyes are designed to label cell membranes. Original CellBrite™ dyes contain lipophilic carbocyanine dyes (DiB, Neuro-DiO, DiI, or DiD). These dyes accumulate in membrane lipids where they become fluorescent. Like other membrane dyes like VyBrant® DiO, VyBrant® DiI, ViBrant® DiR, CellMask™, CellVue® Claret, or PKH dyes, original CellBrite™ dyes can be fixed with formaldehyde, but are poorly retained after permeabilization with detergents or fixation with alcohol. CellBrite™ NIR dyes are CellBrite™ dyes with near-infrared fluorescence compatible with small animal NIR imaging systems.

CellBrite™ Fix dyes are a novel class of dye for labeling the cell surface. They are fluorogenic dyes that rapidly accumulate in the plasma membrane, where they react covalently with the cell surface. As a result, surface staining is well-retained after permeabilization or methanol fixation, with only a slight increase in intracellular fluorescence compared to formaldehyde fixation alone. CellBrite™ Fix dyes have better water solubility than classic lipophilic dyes, and as a result they yield much more uniform staining compared to lipophilic carbocyanine dyes like DiO and DiI. CellBrite™ Fix dyes are non-toxic and do not readily transfer between cells. They also can be used to stain yeast and bacteria (gram-positive or gram-negative).

Vybrant® and CellMask™ are trademarks of Thermo Fisher Scientific. CellVue® is a registered trademark of Millipore Sigma.

Category: CellBrite™ Cytoplasmic Membrane Stains

← FAQs

Original CellBrite™ Cytoplasmic Membrane Stains are lipophilic carbocyanine dyes. These dyes undergo an increase in fluorescence when they insert into lipid bilayers. Lipophilic carbocyanine dyes stably label the plasma membrane and other intracellular membranes of cells. They also can be used to stain fixed cells or artificial lipid bilayers.

CellBrite™ Fix Membrane Stains are fluorogenic membrane dyes that react covalently with proteins on the cell surface. They have better water solubility than original CellBrite™ dyes and as a result generally give more even staining of the cell surface compared to original CellBrite™ dyes. CellBrite™ Fix are well retained after fixation and permeabiliation or methanol fixation. However, CellBrite™ Fix dyes cannot be used to stain the plasma membrane of cells after they are already fixed (the dyes stain intracellular membranes of fixed cells).

For all membrane stains, if cells are returned to culture after staining, membrane internalization will occur over time, resulting in predominantly intracellular staining.

Category: CellBrite™ Cytoplasmic Membrane Stains

← FAQs

Lipophilic carbocyanine dyes like our original CellBrite™ Cytoplasmic Membrane Stains have been used to stain neuronal cells in culture for several weeks, and in vivo for up to a year. The dyes do not appreciably affect cell viability, and do not readily transfer between cells with intact membranes, allowing cell migration and tracking studies in mixed populations. Over time, the dyes will be internalized and become intracellular as membranes turn over. Long term stability of labeling may vary between cell types, depending on rates of membrane turnover or cell division.

CellBrite™ Fix Membrane Stains label the cell surface covalently. Like original CellBrite™ dyes, they are non-toxic and do not readily transfer between cells, but will be internalized and become intracellular as membranes turn over.

Category: CellBrite™ Cytoplasmic Membrane Stains

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Shipping/Shelf-life

Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions. Expedited shipment may be requested at time of checkout. Please note that products with recommended storage at 4°C or -20°C may ship at ambient temperature. This will not affect product performance. When you receive the product, place it under the recommended storage conditions.