Support

Quick links
Frequently asked questions - categories
Ask a question
Contact Us
+1

CellBrite™ Cytoplasmic Membrane Dyes

CellBrite™ Cytoplasmic Membrane Labeling Kits are designed to label cell cytoplasmic membranes with fluorescent dyes, available in blue, green, orange, and red. The labeling is stable and nontoxic, suitable for long-term tracking of cells.

Product Attributes

Cellular localization

Membrane/cell surface, Membrane/vesicular

Dye Color

CellBrite™ Blue, CellBrite™ Green, CellBrite™ Orange, CellBrite™ Red

For live or fixed cells

For fixed cells, For live/intact cells

Assay type/options

Co-cultures, Extended staining (several days to weeks)

Fixation options

Permeabilize before staining, Fix after staining (formaldehyde), Fix before staining (formaldehyde)

Colors

Blue, Green, Red, Far-red

Dye Color
Size
Catalog #
price
Qty
1 mL
50 assays
Clear selection
Add to wishlist
ADD TO CART
(Please select quantity above )

Wishlist updated! View wishlist

Shipping, Handling & Tax (CA only) not included in price. Learn more

Tax is assessed in the state of California. All fees will be calculated during checkout based on your shipping information and are subject to change.

SPECIAL - Order 5 or more and receive 10% off.

Request a Bulk Quote

Product Description

CellBrite™ Cytoplasmic Membrane Labeling Kits are designed to label cell cytoplasmic membranes with fluorescent dyes, available in blue, green, orange, and red. The labeling is stable and nontoxic, suitable for long-term tracking of cells. By combining multiple CellBrite™ Cytoplasmic Membrane Stains, one can label multiple cell populations with different colors for studies of cell-cell interactions.

CellBrite™ stains are based on lipophilic carbocyanine dyes: CellBrite™ Blue is based on DiB, CellBrite™ Green is based on Neuro-DiO, CellBrite™ Orange is based on DiI, and CellBrite™ Red is based on DiD. Carbocyanine dyes label cytoplasmic membrane and intracellular membrane structures efficiently and permanently. They have been used as tracers in cell–cell fusion, cellular adhesion, and migration applications due to their properties of low cytotoxicity and high resistance to intercellular transfer.

Biotium’s CellBrite™ Cytoplasmic Membrane Dyes are dye delivery solutions that can be added directly to normal culture media to uniformly label suspended or adherent cells in culture. CellBrite™ dyes allow cell populations to be marked in distinctive fluorescent colors for identification after mixing. Double labeling can identify cells that have fused or formed stable clusters.

We also offer CellBrite™ NIR Cytoplasmic Membrane Dyes with emission in the far-red/near-infrared region for imaging by either microscopy or near-infrared imaging devices.

Kits include:

  • CellBrite™ fluorescent membrane dye pre-dissolved in an optimized staining solution*
  • Loading buffer (CellBrite™ Blue only)

Find the Right Stain for Your Application

CellBrite™ dyes can be used to stain live cells or formaldehyde-fixed cells. Live cells can be fixed in formaldehyde after staining as well, but the staining has poor tolerance for methanol fixation or detergent permeabilization. However, permeabilizing fixed cells before staining with CellBrite™ dyes can give good results, see our Tech Tip: Combining Lipophilic Membrane Dyes with Immunofluorescence. For membrane stains that withstand fixation with either formaldehyde or methanol, as well as detergent permeabilization after staining, see our CellBrite™ Fix Membrane Stains and MemBrite™ Fix Cell Surface Staining Kits.

CellBrite™ dyes do not stain yeast or bacteria. See our Cellular Stains Table for more information on how our dyes stain various organisms.

CellBrite™ dyes are non-toxic and stain cells very stably. They can be used to track cells for days to weeks. However, over time the dyes will be internalized by endocytosis, resulting in labeling of intracellular vesicles. By 24 hours after staining, the dyes will no longer outline the plamsa membrane, but will be localized inside the cell. For long-term visualization of cell morphology in culture, our stable, non-toxic ViaFluor® SE Cell Proliferation Kits may be a more suitable alternative. ViaFluor® SE dyes covalently label cells throughout the cytoplasm and can be tracked for several days or longer by microscopy or flow cytometry. See our Tech Tip: Using ViaFluor® SE Stains for Cell Tracing and Co-Culture to learn more.

Product Information

Catalog No.Membrane DyeAbs/Em
30024CellBrite™ Blue 366/441 nm
30021CellBrite™ Green 484/501 nm
30022CellBrite™ Orange549/565 nm
30023CellBrite™ Red644/665 nm

* CellBrite™ Green, CellBrite™ Orange, and CellBrite™ Red are supplied in ethanol. CellBrite Blue dye (DiB Labeling Solution) was previously supplied as a solution in ethanol. The dye is now formulated in DMSO for better solubility.

References

1. J Cell Biol 103, 171 (1986).
2. J Cell Biol 135,63 (1996).
3.Annals of Anatomy (2014), http://dx.doi.org/10.1016/j.aanat.2014.05.038
4. Veterinary and Comparative Oncology (2016), doi: 10.1111/vco.12236

Cat#30024 CellBrite Blue
1. European Journal of Medicinal Chemistry (2017), https://doi.org/10.1016/j.ejmech.2017.11.004
2. Journal of the American Chemical Society (2018), doi:10.1021/jacs.7b12408

 

You may also like…

    MitoView™ Dyes

    MitoView™ dyes are fluorogenic mitochondrial stains for live cells. The dyes rapidly accumulate in mitochondria and can be imaged without washing. They are available with blue, green, far-red, and near-infrared fluorescence.

    NucSpot® Live Cell Nuclear Stains

    NucSpot® Live Cell Nuclear Stains are cell-membrane permeable DNA dyes that specifically stains nuclei in live or fixed cells. They have excellent specificity for DNA without the need for a wash step, and they have low toxicity for live cell imaging.

    ViaFluor® Live Cell Microtubule Stains

    ViaFluor® Live Cell Microtubule Stains are cell-permeable taxol probes for imaging the microtubule cytoskeleton in live cells. Available with blue, green, or far-red fluorescence.

    LysoView™ Dyes

    LysoView™ dyes label lysosomes in live cells, and are available with blue, green, visible red, and far-red fluorescence. Far-red LysoView™ 650 is compatible with super-resolution imaging by STED and SIM. Note: LysoView™650 (70059 and 70059-T) is temporarily unavailable.

    ViaFluor® SE Cell Proliferation Kits

    ViaFluor® SE Cell Proliferation Kits use amine-reactive dyes to covalently label the cell cytoplasm, and are used to monitor cell division by flow cytometry. The dyes also can be used to stably label cells to image cell morphology, or to track cell populations in mixed co-culture experiments. The labeling withstands fixation and permeabilization for immunostaining.

    Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells

    A convenient assay for staining live (green) and dead (red) cells within the same cell population by flow cytometry or fluorescence microscopy