CELLDATA RNAstorm™ 2.0 FFPE RNA Extraction Kit

Superior RNA extraction from FFPE (paraffin) tissue sections with 3 to 30-fold increase in yield, and 2-fold increase in DV200 (RNA longer than 200 nucleotides) compared to other methods.

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CELLDATA RNAstorm™ 2.0 FFPE RNA Extraction Kit

CELLDATA RNAstorm™ 2.0 FFPE RNA Extraction Kit - Image 2

Comparison of RNA recovery by quantitative RT-PCR from FFPE tissues. “Q” represents a competitive commercial FFPE extraction kit.

CELLDATA RNAstorm™ 2.0 FFPE RNA Extraction Kit - Image 3

Increased DV200 values are observed for RNA extracted using the RNAstorm™ kit relative to a popular commercial kit. The DV200 represents percentage of RNA with length greater than 200 nt, as measured using an Agilent Bioanalyzer RNA 6000 Nano Kit.

An exemplary overlay of BioAnalyzer traces obtained using RNA from the same sample as seen with the RNAstorm™ kit and Kit Q is shown below.

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Product Description

Formalin-fixed tissue samples are a challenge for RNA extraction, often resulting in low yields of amplifiable RNA and poor performance in subsequent steps involving enzymatic manipulation, including reverse transcription and sequencing library preparation. Powered by proprietary CAT5™ technology, the RNAstorm™ extraction kit enhances the removal of formaldehyde-induced damage and provides RNA with higher yield and integrity as shown by a higher RIN score and higher DV200 (% RNA >200nt). For RNA with greater amplifiability in applications such as RNA-seq, qPCR, microarray, or other gene expression analysis, the RNAstorm™ 2.0 FFPE RNA Extraction kit is your best chance for success.

CAT5™ technology for chemical reversal of formaldehyde crosslinking
Extract higher yields of amplifiable RNA compared to other methods
Milder conditions and no harsh solvents
Higher quality RNA with less fragmentation
Better results in downstream analysis like PCR, microarray, or next-generation sequencing
Simple spin column-based workflow

Note: This kit is a replacement for catalog number CD501. It uses a modified deparaffinization protocol with a new Dewaxing Solution (provided in the kit). The new kit retains the same proprietary CAT5™ catalytic technology for highly efficient reversal of formaldehyde crosslinks as the original kit.

See the product table below for our complete line of RNAstorm™ and DNAstorm™ extraction kits for FFPE tissues. We also offer the RNAstorm™ RNA Isolation Kit for purifying RNA from fresh or frozen cells/tissue samples, and the ExoBrite™ EV Total RNA Isolation Kit for isolate RNA from extracellular vesicles.

Advantages of CAT5™ Technology for RNA Extraction

Higher Yields of Amplifiable RNA

Significant improvements in RNA yield and quality (as measured by amount of amplifiable RNA) are seen using a prototype of the RNAstorm™ kit featuring CAT5™ technology on FFPE samples from various tissues and with collection dates ranging from 1976 to 2015 compared to a competitor kit.

Comparison of RNA recovery by quantitative RT-PCR from FFPE tissues. “Q” represents a competitive commercial FFPE extraction kit.

Higher Integrity RNA

Increased DV200 values are observed for RNA extracted using the RNAstorm™ kit relative to a popular commercial kit. The DV200 represents percentage of RNA with length greater than 200 nt, as measured using an Agilent Bioanalyzer RNA 6000 nano kit.

An exemplary overlay of BioAnalyzer traces obtained using RNA from the same sample as seen with the RNAstorm™ kit and Kit Q is shown below.

RNAstorm™ and DNAstorm™ FFPE Extraction Kits

Product Name	Catalog Number	Size
CELLDATA RNAstorm™ 2.0 FFPE RNA Extraction Kit	CD506	50 preps
CELLDATA DNAstorm™ 2.0 FFPE DNA Extraction Kit	CD507	50 preps
CELLDATA DNAstorm™/RNAstorm™ 2.0 Combination Kit	CD508	50 preps
CELLDATA RNAstorm™ 2.0 MagBead FFPE RNA Extraction Kit	CD510-96	96 preps
CELLDATA DNAstorm™ 2.0 MagBead FFPE DNA Extraction Kit	CD509-96	96 preps

Technology

RNA & DNA Extraction Kits

Tech Tips

Tech Tip: Tips & Best Practices for Working with RNA

Product Attributes

Size

50 preps

Storage Conditions

See individual components for storage temperature

Documents, Protocols, SDS and COA

Documents

Molecular Biology Brochure

FFPE Extraction Kits Flyer

Protocols

PI-CD506

SDS

SDS CD506

Citations

Download a curated list of references for CELLDATA DNAstorm™ and RNAstorm™ FFPE Extraction Kits

FAQs

RNAstorm™ and DNAstorm™ FFPE extraction kits

What changed in the CELLDATA RNAstorm™ 2.0 and DNAstorm™ 2.0 FFPE Extraction Kits?

The CELLDATA RNAstorm™ 2.0 FFPE RNA Extraction Kit (Cat. No. CD506) and CELLDATA DNAstorm™ 2.0 FFPE DNA Extraction Kit (Cat. No. CD507) contain a Dewaxing Solution that was reformulated for IP considerations. These kits are direct replacements for the discontinued CELLDATA RNAstorm™ or DNAstorm™ FFPE Extraction Kits (CD501, CD502). The kits continue to utilize Biotium’s proprietary CAT5™ catalytic technology for efficient reversal of formaldehyde crosslinks.

What is the capacity of the spin columns in the DNAstorm™ kit? What is the typical yield?

The maximum capacity of the spin columns in the kit is similar to a standard miniprep column, about 20 ug of DNA. However, the expected yield from FFPE extraction is much lower, it is very rare to get more than 1-2 ug of DNA per prep.

Can I use the RNAstorm™ and DNAstorm™ kits to extract both RNA and DNA from the same sample?

Yes, the RNAStorm™ and DNAStorm™ FFPE kits may be used sequentially. The steps below will allow the protocol to be adapted to extract both RNA and DNA from one sample. You can also download this information in an app note.

Begin by extracting the sample according to the RNAstorm™ kit protocol with the following modifications:

Perform step 3 (normally a 2 hour incubation) for only 30 minutes at 72?C. See note below regarding possible optimization of this step.
Perform steps 4 and 5 of the RNAstorm™ protocol as directed, but do not discard the pellet (which contains the DNA) in step 5.
Transfer the supernatant (which contains the RNA) to a new tube as instructed in step 6.
Continue to incubate the supernatant for another 1.5 hours at 72?C (2 hours total including the initial 30 minutes), then proceed with step 7 of the RNAstorm™ protocol (add Binding Buffer) and all remaining steps as instructed.
Use the pellet from step 2, which contains DNA, as input for step A5 (or B8, depending on deparaffinization choice) of the DNAstorm™ kit manual.
Continue with step A5 (or B8) of the DNAstorm™ protocol by adding 200 µL of CAT5™ Buffer to the pellet, then continue as instructed by the DNAstorm™ protocol.

Note: the initial incubation period can be adjusted depending on relative DNA and RNA yields. If the RNA yield is high but the DNA yield is low, reduce the incubation time in step 3 (no less than 15 mins). If the DNA yield is good but the RNA yield is low, increase the incubation time in step 3 (no more than 2 hours).

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