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Fluo-3, AM Ester, 1 mM in Anhydrous DMSO

Membrane-permeant AM ester form of Fluo-3 that can be loaded into cells via incubation. Because of the relatively low water solubility of the AM ester, the mild detergent Pluronic F-127 (catalog no. 59004) is often used as a dispersing agent to facilitate loading.

Product Attributes

CAS number

121714-22-5

Applications

Calcium indicators

Cell permeability

Membrane permeant

Indicator type

Non-ratiometric

Size
Catalog #
price
Qty
1.0 mL
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Product Description

Membrane-permeant AM ester form of Fluo-3 that can be loaded into cells via incubation. Because of the relatively low water solubility of the AM ester, the mild detergent Pluronic F-127 (catalog no. 59004) is often used as a dispersing agent to facilitate loading. Fluo-3 AM ester itself does not bind Ca2 , but it is readily hydrolyzed to fluo-3 by endogenous esterases once the dye is inside the cells. An important application of Fluo-3 AM ester is its use in high throughput drug screening. For information on fluo-3, see #50010.

  • Orange red solid soluble in DMSO
  • Store -20°C and protect from light, especially when in solution
  • C51H50Cl2N2O23
  • MW: 1129.9
  • [121714-22-5]

 

References

n/a

 

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