Fluo-3, Pentaammonium Salt
Fluo-3, pentaammonium is membrane-impermeant, but can be loaded into cells via microinjection or scrape loading.
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Product Description
Fluo-3, pentaammonium is membrane-impermeant, but can be loaded into cells via microinjection or scrape loading.
- Kd: 450 nM
- λEx/λEm (low or high [Ca2+]) = 506/526 nm
- ε (506 nm) = 86,000 M-1cm-1
- Orange red solid soluble in DMSO and water (pH >6)
- Store at 4°C and protect from light, especially when in solution
- C36H25Cl2N7O13
- MW: 854.7
- [134907-84-9]
BAPTA-based ion indicators like Fluo-3 have been shown to be fixable in situ by EDC/EDAC (cat# 59002). The fixation of indicator dyes is useful for downstream immunofluorescence and IHC studies (Cell Calcium 1997, 21(3), 175).
References
1. Cell Calcium 13, 29 (1992).
2. Biochem J 269, 513 (1990).
3. Biochem Biophys Res Comm 171, 102 (1990).
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