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MCB Glutathione Detection Kit

MCB Glutathione Detection Kit utilizes a thiol-reactive dye monochlorobimane (MCB), which is essentially nonfluorescent until it reacts with a thiol to form a blue fluorescent product.

Product Attributes

Apoptosis/viability marker

Metabolic activity

For live or fixed cells

Cell lysis required

Detection method/readout

Microplate reader (fluorescence)

Assay type/options

Endpoint assay

Colors

Blue

Size
Catalog #
price
Qty
100 assays
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Product Description

Diminished cellular glutathione (GSH) level occurs at the early stage of mitochondrion-associated apoptosis pathway due to GSH efflux. GSH depletion further leads to cytochrome c release and caspase 3 induction (1,2). MCB Glutathione Detection Kit utilizes a thiol-reactive dye monochlorobimane (MCB), which is essentially nonfluorescent until it reacts with a thiol to form a blue fluorescent product (λAbsEm = 380/461 nm). By incubating cell lysate with MCB, the intensity of the fluorescent signal generated from the assay reflects the amount of GSH present in the cells. Fluorescence can easily be detected using a fluorometer or 96-well fluorometric plate reader with approximately 1 hour assay time.

Kit Components:

  • Cell lysis buffer
  • Monochlorobimane (MCB)
  • GST enzyme

References

1. FASEB J 12 (6), 479 (1998).

2. Biochem Soc Trans 28, 56 (2000).

 

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