Support & Resources

+1

MTT Cell Viability Assay Kit

MTT Cell Viability Assay Kit provides a simple method for determining live cell numbers using a standard colorimetric plate readers. Determination of live cell numbers is often used to assess the rate of cell proliferation and cytotoxicity caused by drugs and cytotoxic agents.

Product Attributes

Apoptosis/viability marker

Metabolic activity

For live or fixed cells

Cell lysis required

Assay type/options

Endpoint assay, No-wash staining, Real-time imaging, Homogenous assay

Detection method/readout

Microplate reader (absorbance)

Size
Catalog #
price
Qty
1,000 assays
Clear selection
Add to wishlist
ADD TO CART
(Please select quantity above )

Wishlist updated! View wishlist

Shipping, Handling & Tax (CA only) not included in price. Learn more

Tax is assessed in the state of California. All fees will be calculated during checkout based on your shipping information and are subject to change.

SPECIAL - Order 5 or more and receive 10% off.

Request a Bulk Quote

Protocols
SDS

Product Description

The MTT Cell Viability Assay Kit provides a simple method for determining live cell numbers using a standard colorimetric plate readers. Determination of live cell numbers is often used to assess the rate of cell proliferation and cytotoxicity caused by drugs and cytotoxic agents. Among all non-radioactive viability assays, MTT assay developed by Mossman is one of the most versatile and popular assays. MTT is a tetrazolium salt that is turned into a purple formazan product after reduction by mitochondrial enzymes that are only present in metabolically active live cells, not in dead cells. The amount of formazan product generated is proportional to the number of living cells in the sample. At the end of the assay, the cells containing the formazan product are solubilized and then photometrically quantified at 570 nm.

Also see the XTT Cell Viability Assay Kit, which does not require solubilization before absorbance measurement.

References

1. J Immunol Meth 174, 311 (1994).

2. J Immunol Meth 145, 199 (1991)

3. J Immunol Meth 131, 165 (1990).

4. Cancer Res 48, 598 (1988).

 

You may also like…