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Podocalyxin Monoclonal Mouse Antibody (2A4)

Podocalyxin is a member of the CD34 transmembrane sialomucin family. It is expressed on kidney podocyte foot projections. It is also expressed on vascular endothelia, hematopoietic progenitors and a subset of neurons.

Product Attributes

Antibody number

0483

Reactivity (target)

Podocalyxin

Antibody type

Primary

Host species

Mouse

Clonality

Monoclonal

Clone

2A4

Isotype

IgM

Molecular weight

165-170 kDa

Synonyms

PODXL, PDXL, GCTM-2; Gp200; PCLP1; Pcx; Podocalyxin-like protein 1

Human gene symbol

PODXL

Entrez gene ID

5420

SwissProt

O00592

Unigene

732423

Immunogen

A recombinant protein fragment containing the intracellular, transmembrane, and part of the extracellular domain of human podocalyxin

Cellular localization

Cytoplasmic, Membrane/cell surface

Species reactivity

Rabbit, Human, Rat

Applications

Immunofluorescence, Immunohistology (formalin), Flow cytometry

Application notes

Immunohistology formalin-fixed 0.5-1 ug/mL, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes, Flow Cytometry 0.5-1 ug/million cells/0.1 mL, Immunofluorescence 1-2 ug/mL, Optimal dilution for a specific application should be determined by user

Positive control

HeLa, Raji, Jurkat cells, Angiosarcoma, Breast, Prostate, Liver, Pancreatic, Kidney

Shipping condition

Room temperature

Storage Conditions

Store at 2 to 8 °C, Note: store BSA-free antibodies at -10 to -35 °C

Shelf life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Regulatory status

For research use only (RUO)

Research areas

Developmental biology

Cell/tissue expression

Kidney

Conjugation
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Product Description

Podocalyxin is a member of the CD34 transmembrane sialomucin family. It is highly on kidney podocyte foot projections and plays essential roles in kidney development and homeostasis, blood filtration and urine formation. It is also expressed on vascular endothelia, hematopoietic progenitors and a subset of neurons. Overexpression of podocalyxin may be linked to more aggressive tumor behavior. Podocalyxin antibody can identify podocytes in the urine (podocyturia) that may indicate glomerular disease, pre-eclampsia, and other kidney pathology.

This antibody is available purified with BSA/azide at 200 ug/mL, or BSA/azide-free at 1 mg/mL.

References

Kershaw DB et. al. J Biol Chem 272:15708-15714 (1997). | Sassetti C et. al. J Exp Med 187:1965-1975. 3. Nielsen JS et. al. J Am Soc Nephrol 20:1669-1676 (2009). |

 

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