Monoclonal anti Podocalyxin (3D3)

BNUB0427-0
BNUB0427-1BNUB0427-2BNUB0427-3
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Product Description

Podocalyxin is a member of the CD34 transmembrane sialomucin family. It is over-expressed on the podocyte foot projections and plays essential roles in kidney development and homeostasis, blood filtration and urine formation. It is also expressed on vascular endothelia, hematopoietic progenitors and a subset of neurons. Overexpression of podocalyxin may be linked to more aggressive tumor behavior. Podocalyxin antibody can identify podocytes in the urine (podocyturia) that may indicate glomerular disease, pre-eclampsia, and other kidney pathology.

Kershaw DB et. al. J Biol Chem 272:15708-15714 (1997). | Sassetti C et. al. J Exp Med 187:1965-1975. 3. Nielsen JS et. al. J Am Soc Nephrol 20:1669-1676 (2009). |

Additional Information

Conjugation

Purified with BSA, 0.2 mg/mL, Purified, BSA-free, 1 mg/mL, CF405S, CF405M, CF488A, CF543, CF555, CF568, CF594, CF640R, CF647, CF660R, CF680, CF680R, CF770, Biotin, R-PE, APC, Per-CP, Alkaline Phosphatase, HRP

Size

50 uL, 100 uL, 250 uL, 500 uL

Supplied As

Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide, Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA

Antibody type

Primary

Host species

Mouse

Reactivity (target)

Podocalyxin (3D3)

Clonality

Monoclonal

Clone

3D3

Isotype

IgG1

Molecular weight

165-170 kDa

Synonyms

PODXL, PDXL, GCTM-2; Gp200; PCLP1; Pcx; Podocalyxin-like protein 1

Entrez gene ID

5420

SwissProt

O00592

Immunogen

A recombinant protein fragment containing the intracellular, transmembrane, and part of the extracellular domain of human podocalyxin

Unigene

732423

Species reactivity

Human, Rabbit, Rat

Applications

Immunoprecipitation, Flow cytometry, Immunofluorescence, Immunohistology (formalin), Western

Cellular localization

Cell surface and cytoplasmic

Application notes

Flow Cytometry (0.5-1 ug/million cells/0.1 mL), Immunofluorescence 1-2 ug/mL, Immunohistology formalin-fixed 0.5-1 ug/mL for 30 min at RT, Immunoprecipitation 1-2 ug/500 ug protein, Optimal dilution for a specific application should be determined by user, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes, Western blotting 0.5-1 ug/mL

Positive control

HeLa, Raji, Jurkat cells, Angiosarcoma, Breast, Prostate, Liver, Pancreatic, Kidney

MSDS (PDF):MSDS Primary antibodies

Protocol (PDF): Protocols for antibody-based detection

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