Support & Resources

+1

Podocalyxin Monoclonal Mouse Antibody (4F10)

Product Attributes

Antibody number

#0482

Antibody reactivity (target)

Podocalyxin

Antibody type

Primary

Host species

Mouse

Clonality

Monoclonal

Clone

4F10

Isotype

IgM

Molecular weight

165-170 kDa

Synonyms

PODXL, PDXL, GCTM-2; Gp200; PCLP1; Pcx; Podocalyxin-like protein 1

Human gene symbol

PODXL

Entrez gene ID

5420

SwissProt

O00592

Unigene

732423

Immunogen

A recombinant protein fragment containing the intracellular, transmembrane, and part of the extracellular domain of human podocalyxin

Verified antibody applications

IHC (FFPE) (verified)

Antibody target cellular localization

Plasma membrane

Species reactivity

Human, Rabbit, Rat

Antibody application notes

Immunohistology formalin-fixed 0.5-1 ug/mL, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes, Flow Cytometry 0.5-1 ug/million cells/0.1 mL, Immunofluorescence 1-2 ug/mL, Optimal dilution for a specific application should be determined by user

Positive control

HeLa, Raji, Jurkat cells, Angiosarcoma, Breast, Prostate, Liver, Pancreatic, Kidney

Shipping condition

Room temperature

Storage Conditions

Store at 2 to 8 °C, Note: store BSA-free antibodies at -10 to -35 °C

Shelf life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Regulatory status

For research use only (RUO)

Antibody/conjugate formulation

Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA, Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide

Antibody research areas

Developmental biology

Cell/tissue expression

Kidney

Conjugation
Don’t see what you’re looking for? Contact us for more conjugation options.
Note: Please be advised that primary antibodies with catalog numbers that begin with “BN” will only be offered conjugated to CF®405S, CF®488A, CF®568, CF®594, CF®640R, CF®647, and biotin as of 5/27/2022. However, previously available primary antibody conjugates that are in stock will remain available until inventory is exhausted. Please view the list of available conjugates on the product page. We also recommend our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with all CF® Dyes, fluorescent proteins, tandem dyes, and enzyme reporters. We apologize for the inconvenience.
Size
Catalog #
price
Qty
50 uL
100 uL
500 uL
Clear selection
Add to wishlist
ADD TO CART
(Please select quantity above )

Wishlist updated! View wishlist

Product Description

Podocalyxin is a member of the CD34 transmembrane sialomucin family. It is highly on kidney podocyte foot projections and plays essential roles in kidney development and homeostasis, blood filtration and urine formation. It is also expressed on vascular endothelia, hematopoietic progenitors and a subset of neurons. Overexpression of podocalyxin may be linked to more aggressive tumor behavior. Podocalyxin antibody can identify podocytes in the urine (podocyturia) that may indicate glomerular disease, pre-eclampsia, and other kidney pathology.

This antibody is available purified with BSA/azide at 200 ug/mL, or BSA/azide-free at 1 mg/mL.

 

You may also like…