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TGF-Alpha Monoclonal Mouse Antibody (1E8-G6)

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Product Attributes

Antibody number

#0785

Antibody reactivity (target)

TGF-Alpha

Antibody type

Primary

Host species

Mouse

Clonality

Monoclonal

Clone

1E8-G6

Isotype

IgM, kappa

Molecular weight

6 kDa

Synonyms

EGF-like TGF; ETGF; TFGA; TGF Type 1; TGFA; Wa1; Waved 1

Human gene symbol

TGFA

Entrez gene ID

7039

SwissProt

P01135

Unigene

170009

Immunogen

A 10-amino acid synthetic peptide (aa34-43; PPVAAAVVSH) from human TGF?.

Antibody target cellular localization

Secreted (extracellular)

Verified antibody applications

IHC (FFPE) (verified)

Species reactivity

Human, Rabbit, Zebrafish

Antibody application notes

Immunohistology formalin-paraffin 2-4 ug/mL, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes, Flow Cytometry 0.5-1 ug/million cells/0.1 mL, Immunofluorescence 1-2 ug/mL, Optimal dilution for a specific application should be determined by user

Positive control

Jurkat or Ramos cells. Testicular Carcinoma

Shipping condition

Room temperature

Storage Conditions

Store at 2 to 8 °C, Note: store BSA-free antibodies at -10 to -35 °C

Shelf life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Regulatory status

For research use only (RUO)

Antibody/conjugate formulation

Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA, Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide

Antibody research areas

Signal transduction

Conjugation
Don’t see what you’re looking for? Contact us for more conjugation options.
Note: Please be advised that primary antibodies with catalog numbers that begin with “BN” will only be offered conjugated to CF®405S, CF®488A, CF®568, CF®594, CF®640R, CF®647, and biotin as of 5/27/2022. However, previously available primary antibody conjugates that are in stock will remain available until inventory is exhausted. Please view the list of available conjugates on the product page. We also recommend our easy-to-use Mix-n-StainTM Antibody Labeling Kits for labeling antibodies with all CF® Dyes. We apologize for the inconvenience.
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Product Description

This antibody reacts with the TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. The staining with this MAb is completely blocked by the peptide used for raising this antibody. TGF-beta (aa50) is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.

This antibody is available purified with BSA/azide at 200 ug/mL, or BSA/azide-free at 1 mg/mL.

 

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