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TGF-Alpha Monoclonal Mouse Antibody (TG86 + P/T1)

This antibody reacts with the TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin.

Product Attributes

Antibody number

0790

Reactivity (target)

TGF-Alpha

Antibody type

Primary

Host species

Mouse

Clonality

Monoclonal

Clone

TG86 + P/T1

Isotype

IgM

Molecular weight

6 kDa

Synonyms

EGF-like TGF; ETGF; TFGA; TGF Type 1; TGFA; Wa1; Waved 1

Human gene symbol

TGFA

Entrez gene ID

7039

SwissProt

P01135

Unigene

170009

Immunogen

A 10-amino acid synthetic peptide (aa34-43; PPVAAAVVSH) from human TGF? (TG86 & P/T1)

Cellular localization

Cytoplasmic, Secreted (extracellular)

Species reactivity

Zebrafish, Rabbit, Human

Applications

Immunofluorescence, Immunohistology (formalin), Flow cytometry

Application notes

Immunohistology formalin-paraffin 2-4 ug/mL, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes, Flow Cytometry 0.5-1 ug/million cells/0.1 mL, Immunofluorescence 1-2 ug/mL, Optimal dilution for a specific application should be determined by user

Positive control

Jurkat or Ramos cells. Testicular Carcinoma

Shipping condition

Room temperature

Storage Conditions

Store at 2 to 8 °C, Note: store BSA-free antibodies at -10 to -35 °C

Shelf life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Regulatory status

For research use only (RUO)

Research areas

Signal transduction

Conjugation
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Product Description

This antibody reacts with the TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. The staining with this MAb is completely blocked by the peptide used for raising this antibody. TGF-beta (aa50) is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.

This antibody is available purified with BSA/azide at 200 ug/mL, or BSA/azide-free at 1 mg/mL.

 

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