Monoclonal anti TGFalpha (MF9)

BNUB0644-0
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Product Description

This antibody reacts with the C-terminus of TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. The staining with Ab-1 is completely blocked by the peptide used for raising this antibody. TGF? ? (aa50) is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.

Kobrin MS et. al. J Bio Chem, 1986, 261:14414-9. | Kudlow JE et. al. Endocrinology, 1987, 121(4):1577-9. | Kobrin MS et. al. Endocrinology, 1987, 121(4):1412-6. | Kudlow JE et. al. J Bio Chem, 1989, 264(7):3880-

Additional Information

Conjugation

Purified with BSA, 0.2 mg/mL, Purified, BSA-free, 1 mg/mL, CF405S, CF405M, CF488A, CF543, CF555, CF568, CF594, CF640R, CF647, CF660R, CF680, CF680R, CF770, Biotin, R-PE, APC, Per-CP, Alkaline Phosphatase, HRP

Size

50 uL, 100 uL, 250 uL, 500 uL

Supplied As

Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide, Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA

Antibody type

Primary

Host species

Mouse

Reactivity (target)

TGFalpha (MF9)

Clonality

Monoclonal

Clone

MF9

Isotype

IgG2a, kappa

Molecular weight

6 kDa

Synonyms

EGF-like TGF; ETGF; TFGA; TGF Type 1; TGFA; Wa1; Waved 1

Entrez gene ID

7039

SwissProt

P01135

Immunogen

A 17-amino acid synthetic peptide from the carboxyl-terminus of rat TGF?.

Unigene

170009

Species reactivity

Cow, Human, Mouse, Rat

Applications

Flow cytometry, Immunofluorescence, Immunohistology (formalin)

Cellular localization

Cytoplasmic and extracellular (secreted)

Application notes

Flow Cytometry (0.5-1 ug/million cells/0.1 mL), Immunofluorescence 1-2 ug/mL, Immunohistology formalin-fixed 2-4 ug/mL for 30 minutes at RT, Optimal dilution for a specific application should be determined by user, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes

Positive control

Jurkat or Ramos cells. Testicular Carcinoma

MSDS (PDF):MSDS Primary antibodies

Protocol (PDF): Protocols for antibody-based detection

Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions.

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