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TNF-Alpha Monoclonal Mouse Antibody (TNF706)

Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice.

Product Attributes

Antibody number

0706

Reactivity (target)

TNF-Alpha

Antibody type

Primary

Host species

Mouse

Clonality

Monoclonal

Clone

TNF706

Isotype

IgM, kappa

Molecular weight

17 kDa

Human gene symbol

TNF

Entrez gene ID

7124

SwissProt

P01375

Unigene

241570

Immunogen

Recombinant N-terminal fragment of human TNF-?

Cellular localization

Cytoplasmic, Secreted (extracellular)

Species reactivity

Cat, Dog, Zebrafish, Mouse, Rabbit, Human, Rat

Applications

Immunofluorescence, Immunohistology (formalin), Flow cytometry

Application notes

Immunohistology formalin-paraffin 2-4 ug/mL, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris buffer with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes, Flow Cytometry 0.5-1 ug/million cells/0.1 mL, Immunofluorescence 1-2 ug/mL, Optimal dilution for a specific application should be determined by user

Positive control

HeLa, HL-60, or A431 cells. Macrophages in lymph node or Tonsil

Shipping condition

Room temperature

Storage Conditions

Store at 2 to 8 °C, Note: store BSA-free antibodies at -10 to -35 °C

Shelf life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Regulatory status

For research use only (RUO)

Research areas

Cytokines, Immunology

Conjugation
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Product Description

Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five non-covalently linked units, but shows a single 17 kDa band following SDS PAGE under non-reducing conditions. TNF alpha is closely related to the 25 kDa protein Tumor Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production.

This antibody is available purified with BSA/azide at 200 ug/mL, or BSA/azide-free at 1 mg/mL.

References

McLaughlin PJ; Elwood NJ; Ramadi LT, Pica MR, McKenzie IF. Improvement in sensitivity of enzyme-linked immunosorbent assay for tumor necrosis factor. Immunol Cell Biol, 1990, 68:51-5. |

 

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