Monoclonal anti TNF alpha (TNF706 + P/T2)

BNUB0707-0
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Catalog #: BNUB0707
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Product Description

TNF alpha is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha exists as a multimer of two, three, or five non-covalently linked units, but shows a single 17kDa band following SDS PAGE under non-reducing conditions. TNF alpha causes cytolysis of certain transformed cells. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex.

Bebok Z; Markus B; Nemeth P. Prognostic relevance of transforming growth factor alpha (TGF-alpha) and tumor necrosis factor alpha (TNF-alpha) detected in breast cancer tissues by immunohistochemistry. Breast Cancer Research and Treatment, 1994, 29(3):229-35

Additional Information

Conjugation

Purified with BSA, 0.2 mg/mL, Purified, BSA-free, 1 mg/mL, CF405S, CF405M, CF488A, CF543, CF555, CF568, CF594, CF640R, CF647, CF660R, CF680, CF680R, CF770, Biotin, R-PE, APC, Per-CP, Alkaline Phosphatase, HRP

Size

50 uL, 100 uL, 250 uL, 500 uL

Supplied As

Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide, Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA

Antibody type

Primary

Host species

Mouse

Reactivity (target)

TNF alpha (TNF706 + P/T2)

Clonality

Monoclonal

Clone

TNF706 + P/T2

Isotype

IgM, kappa

Molecular weight

17 kDa

Synonyms
Entrez gene ID

7124

SwissProt

P01375

Immunogen

Recombinant N-terminal fragment of human TNF-? (TNF706); Peptide corresponding to aa115-130 of human TNF-? (P/T2)

Unigene

241570

Species reactivity

Cat, Dog, Human, Mouse, Rabbit, Rat, Zebrafish

Applications

Flow cytometry, Immunofluorescence, Immunohistology (formalin)

Cellular localization

Cytoplasmic and extracellular (secreted)

Application notes

Flow Cytometry (0.5-1 ug/million cells/0.1 mL), Immunofluorescence 1-2 ug/mL, Immunohistology formalin-paraffin 2-4 ug/mL for 30 min at RT, Optimal dilution for a specific application should be determined by user, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris buffer with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes

Positive control

HeLa, HL-60, or A431 cells. Macrophages in lymph node or Tonsil or Histiocytoma

MSDS (PDF):MSDS Primary antibodies

Protocol (PDF): Protocols for antibody-based detection

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