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A ready-to-use luminescent protein gel stain optimized for UV-based fluorescence imaging systems. Non-toxic, with no fixation required.
One-Step Lumitein™ UV Protein Gel Stain is a ready-to-use luminescent red protein gel stain optimized for use on UV transilluminators. With no fixation or wash steps required, One-Step Lumitein™ UV is a great choice for convenience and safety. One-Step Lumitein™ UV can detect as little as approximately 1-10 ng of protein per band.
One-Step Lumitein™ UV gel staining requires only a single 5-30 minute staining step without fixation. Destaining is optional. One-Step Lumitein™ UV can detect as little as approximately 1-10 ng of protein per band depending on the staining method used, although staining intensity varies between proteins. Staining is fully compatible with mass spectrometry and Edman-based sequencing. Gels stained with One-Step Lumitein™ UV Protein Gel Stain can be visualized with a variety of different UV-based fluorescence imaging systems.
The results obtained with One-Step Lumitein™ UV are comparable to those with Oriole™ Fluorescent Gel Stain; however, One-Step Lumitein™ UV has a more convenient protocol, does not contain hazardous solvents, and does not cause gel shrinkage. Furthermore, One-Step Lumitein™ costs significantly less than other fluorescent protein gel stains. One-Step Lumitein™ UV Protein Gel Stain is available in 1 liter bottle or 4 liter Cubitainer® sizes.
One-Step Lumitein™ UV Protein Gel Stain offers safer handling and ease of disposal, because it is an aqueous-based solution that does not contain hazardous methanol or acetic acid. One-Step stains are certified under CCR Title 22 as non-toxic to the environment for drain disposal after a simple pH neutralization step.

Red fluorescent One-Step Lumitein™ UV Protein Gel Stain is designed for imaging using a UV transilluminator. It is an alternative to Oriole™ Fluorescent Gel Stain, but does not contain hazardous solvents and does not cause gel shrinkage.
Please visit our Protein Gel Stains technology page to learn about our other safe and sensitive protein gel stains such as One-Step Lumitein™ and One-Step Blue™, a rapid, easy-to-use, non-toxic alternative to Coomassie staining for visible blue staining.
| Product | Replacement for | Catalog number | Unit size | Product protocol | Safety report |
|---|---|---|---|---|---|
| One-Step Blue® | Coomassie Brilliant Blue stains | 21003-1L | 1L | PI-21003 | One-Step Gel Stains Safety Report |
| One-Step Lumitein™ | SYPRO® Ruby Protein Gel Stain | 21004-1L | 1L | PI-21004 | |
| 21004-4L | 4L | ||||
| One-Step Lumitein™ UV | Oriole™ and Flamingo™ Protein Gel Stains | 21005-1L | 1L | PI-21005 | |
| 21005-4L | 4L |
Nature Biotechnology (2018), doi:10.1038/nbt.4173
Nature Biotechnology (2018), doi:10.1038/nbt.4173
Even though AccuOrange™ buffer does contain SDS, which is required for the dye to bind proteins, the assay is very sensitive to small changes in SDS concentration, and also cannot tolerate non-ionic detergents that form mixed micelles with SDS, like Triton®. Therefore we don’t recommend using the kit for cell lysates or other samples with significant amounts of detergents.
Gels stained with One-Step Blue® can be dried just like gels stained with Coomassie. The stain will not interfere with the detection of radiolabeled proteins.
The AccuOrange™ assay is a fluorescent dye-based assay. The dye binds to proteins primarily through hydrophobic interactions. Proteins denature upon heating; the dye binds to the exposed hydrophobic pockets of the protein after cooling. The free AccuOrange™ dye is fluorogenic due to non-radioactive decay but becomes highly fluorescent due to the rigid conformation inside the pocket.
The AccuOrange™ assay more sensitive than traditional protein quantitation assays such as BCA, Bradford and Lowry, and shows superior linearity and reproducibility than the NanoOrange® protein quantitation assay (Thermo Fisher Sci.), but has low tolerance for detergents like SDS and Triton® X-100.