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Lumitein™ Protein Gel Stain 100X

A luminescent dye designed for detecting proteins in SDS polyacrylamide (SDS-PAGE) gels. As sensitive as silver stain.

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Excitation/Emission

280, 450 (broad)/610 nm

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Product Description

Lumitein™ is a luminescent dye designed for detecting proteins in SDS polyacrylamide (SDS-PAGE) gels, and can also be used to detect proteins in native PAGE gels after an additional SDS incubation step. The stain combines excellent sensitivity, user-friendliness and compatibility with common instruments and downstream analysis. Lumitein™ is as sensitive as silver stain, detecting 1 ng or less protein. For faster, non-toxic alternatives to Lumitein™ for protein gel staining, see our One-Step Lumitein™ and One-Step Blue™ Protein Gel Stains.

  • As sensitive as silver stain, detecting < 1 ng protein.
  • Simple staining procedure.
  • Image with UV gel box (EtBr filter) or laser scanner.
  • Wide linear detection range.
  • Compatible with Downstream Analysis such as mass spec and sequencing.
  • Stable at room temperature.
  • 2, 10, and 50 mL sizes.

Lumitein™ Protein Gel Stain, 100X is an economical concentrated solution. Lumitein™ is also available as a 1X convenient, ready-to-use staining solution (catalog no. 21001).

Lumitein™ sensitivity

Lumitein™ is as sensitive as the best silver stain by detecting 1 ng or less protein (Figure 1). Unlike silver stain, however, Lumitein™ has a linear detection range of at least 3 orders of magnitude. It is among the simplest protein gel stain by staining protein in gels in 90 minutes or less time without a separate fixation step. Lumitein™ has an excitation spectrum that makes detection possible with either a simple UV box or a high-end laser scanner. Moreover, protein gel staining with Lumitein™ is compatible with downstream protein analyses such as mass spectrometry and Edman-based sequencing (Figure 2).

Figure 1. Two-fold serial dilutions of protein marker were separated via SDS-PAGE and then stained with Lumitein™. Imaged with a GE Typhoon Trio using 532 nm excitation and 610BP30 emission filter. Figure 2. 2-D gel of human liver protein lysate stained with Lumitein™. The three circled spots were picked for MS analysis by Applied Biomics, Inc. (Hayward, CA), confirming that Lumitein™ staining is fully compatible with MS analysis.
Lumitein™ vs. Coomassie

Lumitein

Coomassie

Figure 3. PAGE gels containing electrophoretically separated protein marker (loaded in two-fold serial dilution from left to right) were stained with Lumitein™ protein gel stain (left) and Coomassie Blue (right). The Lumitein™-stained gel was imaged using a UV box equipped with EtBr filter (UVP) while the Coomassie-stained gel was imaged using a white light converter (UVP).

References

1. Meth Mol Biol. 869, 621 (2012).
2. Appl Environ Microbiol (2013) doi:10.1128/AEM.01478-13
3. J Mol Biol (2012) doi:10.1016/j.jmb.2012.10.018
4. Environ Microbiol 12, 2190 (2010).
5. Can J Microbiol 55, 304 (2009).
6. Res Microbiol 160, 401 (2009)
7. Org. Biomol. Chem. (2014) doi:10.1039/C4OB00622D
8. PLoS ONE (2014) doi:10.1371/journal.pone.0112874
9. International Journal of Food Microbiology (2015) doi:10.1016/j.ijfoodmicro.2014.12.012
10. BMC Microbiology (2015) doi: 10.1186/s12866-015-0387-7
11. J Biochem (2015) doi: 10.1093/jb/mvv063
12. Bioscience, Biotechnology, and Biochemistry (2016) doi: 10.1080/09168451.2015.1123609
13. Nature Structural & Molecular Biology (2016) doi:10.1038/nsmb.3237
14. Biophysical Journal (2018) doi:10.1016/j.bpj.2018.03.025

 

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