Cell & Organelle Stains

NBD C6-sphingomyelin (6-((N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)hexanoyl)sphingosyl phosphocholine) has been used for following sphingolipid metabolism in cells.

NBD-PE (N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)-1,2-dihexadecanoyl-snglycero-3-phosphoethanolamine, triethylammonium salt) in combination with Rhodamine-DHPE (#60026) or Texas Red®-DHPE (#60027) has been used to study membrane fusion via fluorescence resonance energy transfer (FRET) (1-3). NBD-PE has also been used in photobleaching recovery measurement.

Neuro-DiI was developed at Biotium as an alternative to the widely used fluorescent membrane probe DiI. Neuro-DiI has structural features that may make the probe diffuse faster than DiI on cell membranes and also may result in a more stable labeling.

Neuro-DiI uniformly dissolved in vegetable oil in a formulation optimized for microinjection. Neuro-DiI was developed at Biotium as an alternative to the widely used fluorescent membrane probe DiI.

Biotium developed Neuro-DiO as a replacement for the green fluorescent DiO, which has been found to be difficult to use for neurons and cell suspension due to low solubility, tendency to form aggregates and slow lateral diffusion rate.

Neuro-DiO is a derivative of DiO and has spectral properties nearly identical to that of the latter. However, unlike DiO, Neuro-DiO is structurally more similar to DiI, which is known to be a much better lipophilic neuronal tracer than regular DiO.

Nile Red is a red fluorescent neutral lipid stain.

NAO is a green fluorescent mitochondrial dye whose staining is not dependent on mitochondrial membrane potential.

An improved alternative to 7-AAD showing less bleed-through fluorescence in the PE-Texas Red® channel. Ideal for selective detection of dead cells by flow cytometry.

The fluorescence of octadecyl rhodamine B in membranes is quenched at high dye concentration but is released at dilution. This property makes the dye useful for membrane fusion assays.

A membrane-impermeant nucleic acid intercalator. The dye is commonly used to selectively stain dead cells in a mixed live-dead population and also used as a counterstain in multicolor fluorescent assays.

The styryl dye RH237 is a fast-responding potentiometric probe that is primarily used for functional imaging of neurons.

The styryl dye RH414 is a fast-responding potentiometric probe that is primarily used for functional imaging of neurons.

RH421 is a fast responding membrane potential dye, exhibiting >20% fluorescence change per 100 mV on neuroblastoma cells.

The styryl dye RH795 is a fast-responding potentiometric probe that is primarily used for functional imaging of neurons. The dye is spectrally similar to RH414 (61016), but exhibits different physiological effects during staining. For example, RH414 causes arterial constriction during cortex staining, while RH795 does not (see references 1,2).

Rhodamine 123 is a popular green fluorescent mitochondrial dye that stains mitochondria in living cells in a membrane potential-dependent fashion. It is widely used in flow cytometry studies involving mitochondrial membrane potential.