Cell & Organelle Stains

Di-8-ANEPPQ is a fast-responding membrane potential dye. It is more hydrophobic than Di-2-ANEPEQ and is useful for potential-sensitive retrograde labeling of neurons.

Di-12-ANEPPQ is a fast-responding membrane potential dye. It is more hydrophobic than Di-8-ANEPPQ and is useful for potential-sensitive retrograde labeling of neurons.

ANEPPS dyes are fast responding membrane potential dyes. Compared to Di-4-ANEPPS, Di-8-ANEPPS is better retained in the outer leaflet of cell plasma membranes and thus more suitable for long-term membrane potential studies. Di-8-ANEPPS is also more photostable and less phototoxic than Di-4-ANEPPS

Di-2-ANEPEQ, also called JPW1114, is a fast-responding membrane potential dye. It is highly water-soluble and is usually introduced into cells by microinjection.

DiBAC₄(3) is a translational membrane potential dye that redistributes within the cell membrane when membrane potential changes.

Di-4-ANEPPS is a fast-responding membrane potential dye. Changes in the membrane potential of the cell correlate with changes in the fluorescence excitation intensity of the dye.

Dilinoleyl DiO (1,1′-dilinoleyl-3,3′-oxacarbocynanine perchlorate), also known as FAST DiO™, has identical absorption and emission spectra as DiO (#60011).

Neuro-DiI uniformly dissolved in vegetable oil in a formulation optimized for microinjection. Neuro-DiI was developed at Biotium as an alternative to the widely used fluorescent membrane probe DiI.

Dilinoleyl DiI (1,1′-dilinoleyl-3,3,3′,3′-tetramethylindocarbocynanine perchlorate), also known as FAST DiI™, has identical absorption and emission spectra as DiI (#60010) .

DiI (DiIC18(3); 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine) uniformly dissolved in vegetable oil in a formulation optimized for membrane labeling or cell tracking. Carbocyanine dyes in oil have been widely used for fate mapping, cell lineage studies, pre-labeling cells for transplantation, and neuronal tracing and labeling.

Neuro-DiO is a derivative of DiO and has spectral properties nearly identical to that of the latter. However, unlike DiO, Neuro-DiO is structurally more similar to DiI, which is known to be a much better lipophilic neuronal tracer than regular DiO.

Neuro-DiI was developed at Biotium as an alternative to the widely used fluorescent membrane probe DiI. Neuro-DiI has structural features that may make the probe diffuse faster than DiI on cell membranes and also may result in a more stable labeling.

DiR is a lipophilic, near-infrared fluorescent cyanine dye. The dye is useful for labeling cytoplasmic membranes and has been used for near-infrared in vivo imaging.

DiD (DiIC18(5); 1,1′-dioctadecyl-3,3,3′,3′- tetramethylindodicarbocyanine, 4-chlorobenzenesulfonate salt) is a lipophilic carbocyanine dye similar to DiI, but with longer absorption and emission wavelengths.

Biotium developed Neuro-DiO as a replacement for the green fluorescent DiO, which has been found to be difficult to use for neurons and cell suspension due to low solubility, tendency to form aggregates and slow lateral diffusion rate.

DiA 4-(4-Dihexadecylaminostyryl)-N-methylpyridinium iodide) is a green fluorescent membrane dye which diffuses much faster than DiO in cell membranes. DiA and DiI (60010) have been used together for two color membrane staining.