Cell & Organelle Stains

Hydroxystilbamidine (also called Fluoro-Gold™) has been used extensively as a retrograde tracer for neurons and also a histochemical stain. The 4% solution in deionized H₂O is a convenient, ready-made solution.

RuBP-2S (Ruthenium(bis(bathophenanthroline))(bathophenanthrolinedisulfonate) is a luminescent ruthenium complex similar to RuBPS (80021).

RuBPS (Ruthenium(tris(bathophenanthrolinedisulfonate), tetrasodium salt) is a negatively-charged water-soluble luminescent ruthenium complex. The complex can be efficiently excited by either UV light or visible light in the wavelength range from 400 nm to 500 nm with bright red emission centered around 605 nm.

Like Lucifer Yellow CH, Lucifer Yellow Cadaverine can be used as a fixable fluorescent tracer. In addition, the dye can be used to conjugate to carboxylic acid groups.

Fluorescein biotin (5-((N-(5-(N-(6-(Biotinoyl)amino)hexanoyl)amino)pentyl)thioureidyl) fluorescein) can be used to detect and quantify biotin binding sites and the degree of biotinylation of proteins. Also see biotin-rhodamine 110 (80022) and biotin-4-fluorescein (90062).

This fluorescent biotin derivative is a useful tracer for studying neuronal morphology. The biotin portion of the molecule allows researchers to amplify the fluorescent signal using standard biotin-avidin or biotin-streptavidin biochemistry.

Lucifer Yellow CH lithium salt (LY CH lithium salt) is a widely used polar molecular tracer for studying neuronal morphology. The fluorescent molecule contains a carbohydrazide that allow the molecule to be aldehyde-fixable.

Lucifer Yellow CH potassium salt (LY CH potassium salt) is a widely used polar molecular tracer for studying neuronal morphology. The fluorescent molecule contains a carbohydrazide that allow the molecule to be aldehyde-fixable.

Calcein is a highly negatively charged, water-soluble fluorescein derivative. Its fluorescence is nearly independent of pH in the range between 6.5 and 12.

Hydroxystilbamidine (also called Fluoro-Gold™) has been used extensively as a retrograde tracer for neurons and also a histochemical stain.

DPX is a positively charged quencher that is often used with ANTS (90010) to study membrane fusion or permeability.

SGC5 is a coumarin styryl dye that binds to membranes in a manner similar to the FM® dyes. It becomes highly fluorescent upon insertion into lipid bilayers.

SGC3 is a coumarin styryl dye that binds to membranes in a manner similar to the FM® dyes. It becomes highly fluorescent upon insertion into lipid bilayers.

AM3-25 is a lipophilic styryl dye that has an amine group at the hydrophilic head. The amine group reacts with formaldehyde or glutaldehyde to make the dye fixable.

SynaptoGreen™ C5 is similar to SynaptoGreen™ C4 (70020), except that the lipophilic tail is one carbon longer.

SynaptoGreen™ C18 (equivalent to FM®3-25) is a membrane stain with a chemical structure similar to other nerve terminal dyes, except that it has the longest lipophilic tail (C18). Because of its large size, it is used as a control for ion channel permeability of nerve terminal dyes.